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showing 10 items of 1102 documents

Cocaine abuse determination by ion mobility spectrometry using molecular imprinting

2016

A cocaine-based molecular imprinted polymer (MIP) has been produced by bulk polymerization and employed as selective solid-phase extraction support for the determination of cocaine in saliva samples by ion mobility spectrometry (IMS). The most appropriate conditions for washing and elution of cocaine from MIPs were studied and MIPs were characterized in terms of analyte binding capacity, reusability in water and saliva analysis, imprinting factor and selectivity were established and compared with non-imprinted polymers. The proposed MIP-IMS method provided a LOD of 18μgL-1 and quantitative recoveries for blank saliva samples spiked from 75 to 500μgL-1 cocaine. Oral fluid samples were collec…

AdultMaleAnalyteIon-mobility spectrometry02 engineering and technologyMass spectrometry01 natural sciencesBiochemistryGas Chromatography-Mass SpectrometryPolymerizationAnalytical ChemistryMolecular ImprintingCocaine-Related DisordersYoung AdultCocaineHumansSolid phase extractionSalivaIonsChromatographyChemistryElutionSpectrum AnalysisSolid Phase Extraction010401 analytical chemistryOrganic ChemistryTemperatureMolecularly imprinted polymerWaterGeneral MedicineReference Standards021001 nanoscience & nanotechnology0104 chemical sciencesMicroscopy Electron ScanningSolventsRegression AnalysisFemaleGas chromatography–mass spectrometry0210 nano-technologyMolecular imprintingJournal of Chromatography A
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Magnetic molecularly imprinted polymers for the selective determination of cocaine by ion mobility spectrometry

2018

Abstract Magnetic molecularly imprinted polymers (MMIPs) were prepared for cocaine recognition by bulk polymerization in the presence of magnetic nanoparticles (MNPs). Two reagents (polyethylene glycol (PEG) and 3-(trimethoxysilyl)propyl methacrylate (V)) were used for MNPs modification. MMIPs were characterized and compared in terms of loading capacity, reusability, accuracy and precision for the extraction of cocaine from saliva samples. It was observed that V-MMIPs gave higher physical stability than PEG-MMIPs. Thus, V-MMIP were used for the analysis of cocaine users saliva. The developed procedure based on ion mobility spectrometry (IMS) provided limits of detection and quantification o…

AdultMaleBulk polymerizationPolymersIon-mobility spectrometry02 engineering and technologyPolyethylene glycolTandem mass spectrometryMethacrylate01 natural sciencesBiochemistryPolyethylene GlycolsAnalytical ChemistryMolecular ImprintingMagneticsYoung Adultchemistry.chemical_compoundCocaineIon Mobility SpectrometryHumansOrganosilicon CompoundsSalivaChromatography High Pressure LiquidDetection limitChromatography010401 analytical chemistryOrganic ChemistryMolecularly imprinted polymerSignal Processing Computer-AssistedGeneral Medicine021001 nanoscience & nanotechnology0104 chemical scienceschemistryMethacrylatesMagnetic nanoparticlesFemale0210 nano-technologyJournal of Chromatography A
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The effect of different starting procedures on sprinters' reaction time.

2012

We examined the effect of different false start rules and starters' holding time on athletics sprinters' reaction times. Reaction times from 210 female (25.2 ± 3.8 years) and 361 male (24.8 ± 3.8 years) 100 m sprinters, participating in international championships for seniors from 1997 to 2011, were analysed. Holding time calculations were based on television recordings from the analysed heats (n = 267). Mean reaction times have increased by 20% (0.03 s, P0.001) during a 15 year period due to stricter false start rules. Starters' holding times were between 1.3 and 2.2 s for the analysed competitions. There was a small but significant relationship between reaction time and starters' holding …

AdultMaleCompetitive Behaviorbusiness.industryMovementPhysical Therapy Sports Therapy and RehabilitationAthletic PerformanceFalse startREACTION TIME DECREASEDRunningYoung AdultSex FactorsSprintCompetitive behaviorInterquartile rangeSex factorsReaction TimeMedicineHumansOrthopedics and Sports MedicineFemalebusinessSimulationDemographyHolding timeJournal of sports sciences
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Virtual Planning and 3D printing modeling for mandibular reconstruction with fibula free flap

2017

Background This study was to evaluate the use of virtual planning and 3D printing modeling in mandibular reconstruction and compare the operation time and surgical outcome of this technique with conventional method. Material and Methods Between 2014 and 2017, 15 patients underwent vascularized fibula flap mandibular reconstruction using virtual planning and 3D printing modeling. Titanium plates were pre-bent using the models and cutting guides were used for osteotomies. 15 patients who underwent mandibular reconstruction using fibula flap without aid of virtual planning and 3D printing models were selected as control group. The operation time was recorded and compared in two groups. Accurac…

AdultMaleComputer science3D printingFree flapFree Tissue FlapsPatient Care Planning03 medical and health sciencesYoung Adult0302 clinical medicineOperation timeHumansFibulaMandibular reconstructionGeneral DentistryRetrospective StudiesOrthodonticsbusiness.industryResearchMandible030206 dentistryFibula flapMiddle Aged:CIENCIAS MÉDICAS [UNESCO]OtorhinolaryngologyVirtual planningFibula030220 oncology & carcinogenesisPrinting Three-DimensionalUNESCO::CIENCIAS MÉDICASSurgeryFemaleMandibular ReconstructionOral Surgerybusiness
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Effects of muscle – tendon length on joint moment and power during sprint starts

2005

The aim of this study was to examine the effects of muscle-tendon length on joint moment and power during maximal sprint starts. Nine male sprinters performed maximal sprint starts from the blocks that were adjusted either to 40 degrees or 65 degrees to the horizontal. Ground reaction forces were recorded at 833 Hz using a force platform and kinematic data were recorded at 200 Hz with a film camera. Joint moments and powers were analysed using kinematic and kinetic data. Muscle - tendon lengths of the medial gastrocnemius, soleus, vastus medialis, rectus femoris and biceps femoris were calculated from the set position to the end of the first single leg contact. The results indicated that bl…

AdultMaleKnee JointVastus medialisAccelerationPhysical Therapy Sports Therapy and RehabilitationGeometryKinematicsBicepsRunningTendonsmedicineHumansOrthopedics and Sports MedicineForce platformGround reaction forceMathematicsAustraliaBiomechanicsAnatomyBiomechanical PhenomenaTendonmedicine.anatomical_structureSprintHip JointAnkle JointJournal of Sports Sciences
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Where is the locus of the lowercase advantage during sentence reading?

2016

While most models of visual word identification and reading posit that a word's visual codes are rapidly transformed onto case-invariant representations (i.e., table and TABLE would equally activate the word unit corresponding to "table"), a number of experiments have shown a lowercase advantage in various word identification and reading tasks. In the present experiment, we examined the locus of this lowercase advantage by comparing the pattern of eye movements when reading sentences in lowercase vs. uppercase. Each sentence contained a target word that was high or low in word-frequency. Overall, results showed faster reading times for lowercase than for uppercase sentences. More important,…

AdultMaleLetter caseEye MovementsComputer scienceSpeech recognitionExperimental and Cognitive PsychologyFixation Ocular050105 experimental psychology03 medical and health sciences0302 clinical medicineArts and Humanities (miscellaneous)Developmental and Educational PsychologyHumansSentence reading0501 psychology and cognitive sciencesVisual WordCommunicationbusiness.industry05 social sciencesEye movementGeneral MedicineGazeSemanticsWord lists by frequencyReadingWord identificationPrintingFemaleComprehensionbusiness030217 neurology & neurosurgerySentenceActa Psychologica
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Greater maintenance of bone mineral content in male than female athletes and in sprinting and jumping than endurance athletes: a longitudinal study o…

2020

Abstract Summary We investigated longitudinal changes in tibia bone strength in master power (jumping and sprinting) and endurance (distance) athletes of both sexes. Bone mass but not cross-sectional moment of inertia was better maintained in power than endurance athletes over time, particularly in men and independent of changes in performance. Objective Assessment of effects of sex and athletic discipline (lower limb power events, e.g. sprint running and jumping versus endurance running events) on longitudinal changes in bone strength in masters athletes. Methods We examined tibia and fibula bone properties at distal (4% distal-proximal tibia length) and proximal (66% length) sites using p…

AdultMaleLongitudinal studyAgingosteoporoosiOsteoporosisluu030209 endocrinology & metabolismliikuntamedicine.disease_causeMuskel- und KnochenstoffwechselBone and BonesbiomechanicsRunning03 medical and health sciences0302 clinical medicineJumpingBone DensitymedicineHumansOrthopedics and Sports MedicineTibiaLongitudinal StudiesQuantitative computed tomographyAgedOrthodonticsAged 80 and overmedicine.diagnostic_testbiologyexercisebusiness.industryAthletesBiomechanicsbone geometry030229 sport sciencesMiddle Agedbiology.organism_classificationmedicine.diseaseosteoporosisikääntyminenSprintAthletesageingFemaleOriginal ArticlebiomekaniikkabusinessSportsurheilijat
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Application of mtDNA sequence analysis in forensic casework for the identification of human remains

2000

Abstract In four forensic cases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA con…

AdultMaleMitochondrial DNASequence analysisMinisatellite RepeatsBiologyDNA MitochondrialPolymerase Chain ReactionBone and BonesPathology and Forensic Medicinelaw.inventionlawAge Determination by SkeletonHumansChildPolymerase chain reactionGeneticsmtDNA control regionForensic anthropologySequence Analysis DNADNA FingerprintingHypervariable regionForensic identificationDNA profilingForensic AnthropologyFemaleLawForensic Science International
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Muscle cross-sectional area and voluntary force production characteristics in elite strength- and endurance-trained athletes and sprinters

1989

Seven male elite strength-trained athletes (SA) from different weight categories, six elite sprinters (SPA) and seven elite endurance-trained athletes (EA) volunteered as subjects for examination of their muscle cross-sectional area (CSA), maximal voluntary isometric force, force-time and relaxation-time characteristics of the leg extensor muscles. The SA group demonstrated slightly greater CSA and maximal absolute strength than the SPA group, while the EA group demonstrated the smallest values both in CSA and especially in maximal strength (p<0.05). When the maximal forces were related to CSA of the muscles, the mean value for the SA group of 60.8±10.0 N·cm−2 remained slightly greater than…

AdultMaleMuscle tissuemedicine.medical_specialtyPhysiologyIsometric exerciseIsometric ContractionPhysiology (medical)Internal medicinemedicineHumansOrthopedics and Sports MedicineExercise physiologyExercisebiologybusiness.industryAthletesMusclesMean valuePublic Health Environmental and Occupational HealthGeneral Medicinebiology.organism_classificationCross section (geometry)medicine.anatomical_structureEndocrinologySprintTurnoverPhysical EndurancePhysical therapybusinessEuropean Journal of Applied Physiology and Occupational Physiology
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Clonal evolution in relapsed NPM1-mutated acute myeloid leukemia.

2013

Mutations in the nucleophosmin 1 (NPM1) gene are considered a founder event in the pathogenesis of acute myeloid leukemia (AML). To address the role of clonal evolution in relapsed NPM1-mutated (NPM1mut) AML, we applied high-resolution, genome-wide, single-nucleotide polymorphism array profiling to detect copy number alterations (CNAs) and uniparental disomies (UPDs) and performed comprehensive gene mutation screening in 53 paired bone marrow/peripheral blood samples obtained at diagnosis and relapse. At diagnosis, 15 aberrations (CNAs, n = 10; UPDs, n = 5) were identified in 13 patients (25%), whereas at relapse, 56 genomic alterations (CNAs, n = 46; UPDs, n = 10) were detected in 29 patie…

AdultMaleNPM1MyeloidImmunologyBiologyGene mutationBiochemistrySomatic evolution in cancerPolymorphism Single NucleotideDNA Methyltransferase 3AClonal EvolutionYoung AdultRecurrenceRisk FactorsmedicineHumansDNA (Cytosine-5-)-MethyltransferasesAgedChromosomes Human Pair 13Myeloid leukemiaNuclear ProteinsCell BiologyHematologyMiddle Agedmedicine.diseasePrognosisMinimal residual diseaseDNA FingerprintingLeukemiaETV6Leukemia Myeloid Acutemedicine.anatomical_structureCancer researchFemaleChromosomes Human Pair 9NucleophosminGene DeletionBlood
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