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RESEARCH PRODUCT
Application of mtDNA sequence analysis in forensic casework for the identification of human remains
Peter M. SchneiderChristian RittnerKlaus Bendersubject
AdultMaleMitochondrial DNASequence analysisMinisatellite RepeatsBiologyDNA MitochondrialPolymerase Chain ReactionBone and BonesPathology and Forensic Medicinelaw.inventionlawAge Determination by SkeletonHumansChildPolymerase chain reactionGeneticsmtDNA control regionForensic anthropologySequence Analysis DNADNA FingerprintingHypervariable regionForensic identificationDNA profilingForensic AnthropologyFemaleLawdescription
Abstract In four forensic cases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA control region. After sequencing of the PCR products, the results were compared to the Anderson reference sequence and to putative maternal relatives.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2000-09-09 | Forensic Science International |