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showing 10 items of 1102 documents

Genomic imprinting and neurodevelopment

2021

Abstract During mammalian development, most genes are equally expressed from both the maternal and the paternal alleles. However, a minority of genes known as “imprinted genes’” are expressed or silenced from either the maternal or the paternal homologue, resulting functionally monoallelic. This process known as “genomic imprinting” is essential for normal development and shows tissue and developmental-stage specificity, suggesting a key role in gene dosage fine-tuning. Furthermore, genomic imprinting is highly prevalent in the brain, and many genes with a key role in pre- and postnatal neurodevelopment are expressed in a parent-of-origin specific manner in the central nervous system. This …

Geneticsmedicine.anatomical_structureCentral nervous systemmedicineBiologyAlleleGenomic imprintingGeneImprinting (organizational theory)Gene dosage
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Compatibility of Rhizobial Genotypes within Natural Populations of Rhizobium leguminosarum Biovar viciae for Nodulation of Host Legumes

2003

ABSTRACT Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean ( Vicia faba ) and pea ( Pisum sativum ) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches ( Vicia sativa ) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD , as a marker of the sy…

GenotypeBulk soilBiologymedicine.disease_causeApplied Microbiology and BiotechnologyPlant RootsPolymerase Chain ReactionRhizobium leguminosarumRhizobiaPlant MicrobiologySymbiosisBacterial ProteinsNitrogen FixationBotanyGenotypemedicineSymbiosisSoil Microbiology[SDV.EE]Life Sciences [q-bio]/Ecology environmentRhizosphereRhizobium leguminosarumEcologyPeasfood and beveragesbiology.organism_classificationDNA FingerprintingVicia fabaVicia faba[SDV.EE] Life Sciences [q-bio]/Ecology environmentNitrogen fixationFood ScienceBiotechnologyPlasmids
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High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting.

1999

ABSTRACT For epidemiological studies of Campylobacter infections, molecular typing methods that can differentiate campylobacters at the strain level are needed. In this study we used a recently developed genotyping method, amplified fragment length polymorphism (AFLP), which is based on selective amplification of restriction fragments of chromosomal DNA, for genetic typing of Campylobacter jejuni and Campylobacter coli strains derived from humans and poultry. We developed an automated AFLP fingerprinting method in which restriction endonucleases Hin dIII and Hha I were used in combination with one set of selective PCR primers. This method resulted in evenly distributed band patterns for amp…

GenotypeGenetics and Molecular BiologyCampylobacter coliDeoxyribonuclease HindIIImedicine.disease_causeApplied Microbiology and BiotechnologyCampylobacter jejuniPolymerase Chain ReactionPoultryMicrobiologyRestriction fragmentCampylobacter jejuniGenotypeCampylobacter InfectionsmedicineAnimalsDeoxyribonucleases Type II Site-SpecificGenotypingDNA PrimersGeneticsEcologybiologyCampylobacterfood and beveragesReproducibility of ResultsCampylobacterbiology.organism_classificationDNA FingerprintingBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldDNA profilingCampylobacter colibiology.proteinAmplified fragment length polymorphismFood ScienceBiotechnologyApplied and environmental microbiology
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Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.

2009

ABSTRACT Vibrio vulnificus is a heterogeneous bacterial species that comprises virulent and avirulent strains from environmental and clinical sources that have been grouped into three biotypes. To validate the typing methods proposed to distinguish clinical from environmental isolates, we performed phenotypic (API 20E, API 20NE, and BIOLOG tests) and genetic (ribotyping and DNA polymorphism at several loci) studies with a large strain collection representing different biotypes, origins, and host ranges. No phenotypic method was useful for biotyping or grouping strains with regard to the origin of an isolate, and only the BIOLOG system was reliable for identifying the strains at the species …

GenotypePopulationVibrio vulnificusPublic Health MicrobiologyApplied Microbiology and BiotechnologyMicrobiologyRibotypingGenotypeEnvironmental MicrobiologyCluster AnalysisHumansTypingeducationGenotypingVibrio vulnificusGeneticseducation.field_of_studyPolymorphism GeneticEcologybiologybiology.organism_classification16S ribosomal RNADNA FingerprintingBacterial Typing TechniquesDNA profilingVibrio InfectionsBacteris patògensFood ScienceBiotechnology
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A Life Cycle Environmental Impact Comparison between Traditional, Hybrid, and Electric Vehicles in the European Context

2021

Global warming (GW) and urban pollution focused a great interest on hybrid electric vehicles (HEVs) and battery electric vehicles (BEVs) as cleaner alternatives to traditional internal combustion engine vehicles (ICEVs). The environmental impact related to the use of both ICEV and HEV mainly depends on the fossil fuel used by the thermal engines, while, in the case of the BEV, depends on the energy sources employed to produce electricity. Moreover, the production phase of each vehicle may also have a relevant environmental impact, due to the manufacturing processes and the materials employed. Starting from these considerations, the authors carried out a fair comparison of the environmental …

Geography Planning and DevelopmentTJ807-830Context (language use)life cycle analysisManagement Monitoring Policy and Lawpassenger carTD194-195environmental impactAutomotive engineeringRenewable energy sourcesbattery electric vehicleBattery electric vehicleBattery electric vehicle Environmental impact Hybrid electric vehicle Life cycle analysis Passenger carEnvironmental impact assessmentGE1-350hybrid electric vehicleEnvironmental effects of industries and plantsRenewable Energy Sustainability and the Environmentbusiness.industryFossil fuelEnvironmental sciencesSettore ING-IND/08 - Macchine A FluidoInternal combustion engineCarbon footprintEnvironmental scienceElectricitybusinessEnergy sourceSustainability
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On the Relationship between Jetted Inks and Printed Biopatterns:Molecular-Thin Functional Microarrays of Glucose Oxidase

2009

Arrays of circular spots of glucose oxidase have been obtained on functionalized silicon oxide by piezoelectric inkjet printing and the enzymatic activity toward glucose recognition has been monitored. The addition of glycerol to the molecular ink allows to obtain high spot definition and resolution (tens of micrometers wide; one molecule tall), but in spite of its well-known structural stabilizing properties, in dynamic conditions it may lead to increased protein stresses. The jetting voltage and pulse length have been found to be critical factors for both activity retention and pattern definition. High voltages and pulse lengths results in stress effects along with the loss of activity, w…

GlycerolSiliconSurface PropertiesProtein Array Analysischemistry.chemical_elementNanotechnologyASPERGILLUS-NIGERMicrometreSIO2 SURFACESGlucose Oxidase inkjet printingGlucose OxidaseStructure-Activity RelationshipElectrochemistryGeneral Materials ScienceGlucose oxidaseSilicon oxideSpectroscopybiologyInkwellPulse (signal processing)ChemistryPulse durationSurfaces and InterfacesCondensed Matter PhysicsPiezoelectricityChemical engineeringIMMOBILIZATIONbiology.proteinMicroscopy Electron ScanningInkHIGH HYDROSTATIC-PRESSURE
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The Media and Public Agendas: Testing for Media Effects in Argentina During 2003-2008

2009

In this paper we examine the presence of agenda-setting effects by the print media in Argentina from June 2003 to December 2008. Using previously unavailable monthly data on newspapers mentions we test two hypotheses about the relationship between the different agendas. We find support for the hypothesis that there were media effects during our period of analyisis. More specifically, we find that the total number of newspaper mentions of the President positively influenced public confidence in the government. Finally, there is also evidence of a strong and stable relationship between the total number of economic news and leading economic indicators.

GovernmentEconomic indicatorCointegrationbusiness.industryPrint mediaPolitical scienceAdvertisingPublic opinionbusinessPeriod (music)Test (assessment)NewspaperSSRN Electronic Journal
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Media Control in the Twentieth Century

2015

Media control by which the duplication and distribution of knowledge are under attendance is as old as modern media. Efforts at control emerged rather quickly after the printing press was invented, first inaugurated by the Catholic Church but soon adopted by the state(s). The reasons were similar but the conditions varied from country to country. The decline of government control began in England at the end of the seventeenth century; other countries followed with more or less delay. At the end of the nineteenth century, pre-censorship had generally been abolished in the European countries; however, the twentieth century did not become an era of press freedom as expected. This resulted from…

GovernmentFreedom of the pressmedia_common.quotation_subjectCensorshipAttendanceNew medialaw.inventionPrinting pressState (polity)lawPolitical scienceEconomic historyIdeologymedia_common
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Handwritten Newspapers: An Alternative Medium during the Early Modern and Modern Periods

2019

Graphic communicationTypographyComputer scienceVisual communicationPrint cultureEphemerisGraphic languageNewspaperVisual arts
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Connecting Existing Cemeteries Saving Good Soils (for Livings)

2019

Background: Urban sprawl consumes and degrades productive soils worldwide. Fast and safe decomposition of corpses requires high-quality functional soils, and land use which competes with both agriculture and buildings. On one hand, cremation does not require much land, but it has a high energy footprint, produces atmospheric pollution, and is unacceptable to some religious communities. On the other hand, as exhumations are not practiced, “green burials” require more surface area than current burial practices, so a new paradigm for managing land use is required. Conclusions: In this paper, we propose a concept for ‘green belt communalities’ (i.e., ecological corridors with multiple, yet flex…

Green beltDisposal practiceUrban agglomerationSettore AGR/13 - Chimica AgrariaGeography Planning and Development0211 other engineering and technologies0507 social and economic geographyTerrain02 engineering and technologyUrban environmentsManagement Monitoring Policy and LawFootprintEnvironmental planningLand useRenewable Energy Sustainability and the Environmentbusiness.industryLand take05 social sciencesUrban sprawlUrban area021107 urban & regional planningReligionUrban ecologyGeographySettore AGR/14 - PedologiaAgricultureSettore M-GGR/01 - Geografiabusiness050703 geographySustainability
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