Search results for "probiotique"
showing 6 items of 6 documents
Efficacy and safety profile of LCR35 complete freeze-dried culture in irritable bowel syndrome: A randomized, double-blind study
2011
International audience; AIM: To assess the effects and safety of Lactobacillus casei rhamnosus LCR35 complete freeze-dried culture (LCR35) in patients suffering from irritable bowel syndrome (IBS). METHODS: A randomized, double-blind pilot study was performed in 50 patients complaining of IBS symptoms complying with Rome III criteria. Patients were allocated to receive either LCR35 (n = 25) at a minimum daily dose of 6 × 10(8) colony forming units or placebo (n = 25) for 4 wk. At inclusion, after treatment and 2 wk later, patients completed the IBS severity scale. Change from baseline in the IBS severity score at the end of treatment was the primary efficacy criterion. Changes were compared…
Selection of probiotic strains and improving the survival and functionality of an academic strain, Bifidobacterium bifidum, by changing the redox pot…
2012
The aim of this work was to select rationally a probiotic strain by setting up a screening method as well as study and understand the impact of the redox potential (Eh) and gas bubbling on the survival of Bifidobacterium bifidum in a fermented dairy product. In a first time, we have developed selection techniques of probiotic bacteria on the criteria of viability / vitality (flow cytometry analysis) and on the criteria of functionality (antioxidant). We were able to select strains of industrial interest as well as an academic strain, Bifidobacterium bifidum.Secondly, we have studied effect of modifying redox potential by gas bubbling on the survival of B. bifidum in a fermented dairy produc…
Nouvelles stratégies de stabilisation des bactéries extrêmement sensibles à l’oxygène : cas du probiotique Faecalibacterium prausnitzii.
2016
Intestinal bacteria are of growing interest in the areas of health and wellness [1, 3]. The intestinal ecosystem is characterized by its complex composi-tion and its very low oxygen content. Therefore, nu-merous bacterial species isolated from this environ-ment are extremely sensitive to oxygen. This feature is responsible for the difficulty of implementation of these microorganisms for probiotic large-scale appli-cations, particularly during processing and storage. In this presentation, we present the main steps that have been overcome to conserve and design a stabilization strategy for the bacteria Faecalibacterium prausnitzii, recognized for their anti-inflammatory activities intestinal …
Biogenesis and roles of Lacticaseibacillus casei BL23 membrane vesicles
2023
Gram-positive bacteria of the species Lacticaseibacillus casei (formerly known as Lactobacillus casei) are fermentative bacilli found in dairy products that are known for their anti-inflammatory properties and beneficial effects against pathogens. Like all living organisms, L. casei produces nanostructures of 20 to 400 nm in diameter bounded by a lipid membrane called membrane vesicles (MVs). MVs have been shown to contain various cellular constituents and exert multiple functions. However, despite the growing interest in Gram-positive MVs over the past 10 years, the mechanisms of biogenesis and the roles played by these vesicles remain poorly characterized. In this context, the objectives …
Selection and integration of a new immunomodulatory probiotic strain in a food supplement
2012
Probiotics are live microorganisms that can act on the immune system at specific sites in the gastro-intestinal tract. From a technological point of view, the development of a dietary supplement containing viable and functional probiotics requires the control of the selection and production steps. In a first time, we have developed specific and sensitive screening tests to determine the ability of bacteria to be dried and the origin of its sensitivity. To develop these tests, we investigated the mechanisms involved during drying process. Thus, microorganisms simultaneously undergo osmotic stress and oxidative stress. Results show a strong impact of drying on the survival rate and immunomodu…
Impact of polyphenols and feeding rhythms on the immunomodulation properties of the probiotic bacteria in the gastro-intestinal tract
2021
The human intestinal microbiota is composed of several types of microorganisms, including bacteria, archaea, fungi, unicellular eukaryotes and viruses. Among them, bacteria are the most diverse and abundant with a gene catalog 150 times larger than the genes present in the human genome, which represents a tremendous metabolic potential. These bacteria actively participate in the maintenance of intestinal homeostasis. Dysbiosis of the gut microbiota could be observed at course of many human pathologies, particularly inflammatory diseases intestinal chronic diseases (IBD), such as Crohn's disease (CD) or Ulcerative colitis (UC). These dysbiosis could contribute to the onset and progression of…