Search results for "proteomics."
showing 10 items of 523 documents
Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa.
2016
The formation of the molluscan shell nacre is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell-forming tissue, the mantle. This so-called ‘calcifying matrix’ is a complex mixture of proteins, glycoproteins and polysaccharides that is assembled and occluded within the mineral phase during the calcification process. Better molecular-level characterization of the substances that regulate nacre formation is still required. Notable advances in expressed tag sequencing of freshwater mussels, such as Elliptio complanata and Villosa lienosa , provide a pre-requisite to further characterize bivalve nacre proteins by a proteomic approach. In this…
How subtle is the "terroir" effect? Chemistry-related signatures of two "climats de Bourgogne".
2014
The chemical composition of grape berries is influenced by various environmental conditions often considered to be representative of a "terroir". If grapes from a given terroir are assumed to reflect this origin in their chemical compositions, the corresponding wine should also reflect it. The aim of this work was therefore to reveal the "terroir" expression within the chemodiversity of grapes and related wines, using ultrahigh-resolution mass spectrometry. Grapes and corresponding wines, from two distinct - though very proximate - terroirs of Burgundy were analyzed over three vintages (2010, 2011 and 2012). Ultrahigh-resolution mass spectrometry and ultra-high performan…
Echinostomes: genomics and proteomics
2008
Echinostomes are excellent models to study host-parasite interactions in intestinal helminthiases. In this chapter, data available from genomic and proteomic studies are analyzed in detail to indicate advantages and pitfalls in the research using these organisms. The lack of a genome sequence project and the low number of sequences deposited in the databases not only affect the genomic studies but also debilitate proteomic findings since in most cases, although good spectrometric data can be obtained, the available database often fail to identify the protein from the peptides obtained.
NovelAmycolatopsis balhimycinabiochemical abilities unveiled by proteomics
2014
Amycolatopsis balhimycina DSM5908 is an actinomycete producer of balhimycin, an analogue of vancomycin, the antibiotic of ‘last resort’ against multidrug-resistant Gram-positive pathogens. Most knowledge on glycopeptide biosynthetic pathways comes from studies on A. balhimycina as this strain, among glycopeptide producers, is genetically more amenable. The recent availability of its genome sequence allowed to perform differential proteomic analyses elucidating key metabolic pathways leading to antibiotic production in different growth conditions. To implement proteomic data on A. balhimycina derived from 2-DE approaches and to identify novel components, a combined approach based on protein …
Genomic and Proteomic Analysis of Wine Yeasts
2011
Publisher Summary Saccharomyces cerevisiae is the main microorganism involved in wine fermentation. It has been used as a model organism in molecular biology for many years and is the only wine yeast species for which abundant genomic and proteomic information is available. Most of the techniques currently used in functional genomics and proteomics were initially developed in this yeast. The fact that S. cerevisiae was the first microorganism to be widely used in the development of genome technology allowed other phylogenetically related yeasts to be analyzed subsequently in global sequencing projects, and the use of comparative genomics has since led to important conclusions regarding gene…
The shell matrix and microstructure of the Ram's Horn squid: Molecular and structural characterization.
2020
20 pages; International audience; Molluscs are one of the most diversified phyla among metazoans. Most of them produce an external calcified shell, resulting from the secretory activity of a specialized epithelium of the calcifying mantle. This biomineralization process is controlled by a set of extracellular macromolecules, the organic matrix. In spite of several studies, these components are mainly known for bivalves and gastropods. In the present study, we investigated the physical and biochemical properties of the internal planispiral shell of the Ram's Horn squid Spirula spirula. Scanning Electron Microscope investigations of the shell reveal a complex microstructural organization. The…
Toward the Identification of Two Glycoproteins Involved in the Stomatal Deregulation of Downy Mildew–Infected Grapevine Leaves
2015
SPE Pôle IPM UB; International audience; Stomata remain abnormally opened and unresponsive to abscisic acid in grapevine leaves infected by downy mildew. This deregulation occurs from 3 days post inoculation and increases concomitantly with leaf colonization by the pathogen. Using epidermal peels, we demonstrated that the active compound involved in this deregulation is located in the apoplast. Biochemical assays showed that the active compound present in the apoplastic fluids isolated from Plasmopara viticola infected grapevine leaves (IAF) is a CysCys bridge-independent, thermostable and glycosylated protein. Fractionation guided assays based on chromatography / stomatal response and prot…
How does sulfur deficiency modulate pea response to water stress? Impact on early developing and mature seeds
2018
National audience; Pea (Pisum sativum L.) produces seeds rich in proteins but yield and quality remain unstable across years due to various stresses. Sulfur (S) deficiency and water stress are two abiotic stresses that interact in the current context of climate change and low-input practices, and recent studies suggest an important role for sulfate transport and metabolism in the plant response to water stress. To investigate the interplay between sulfur nutrition and the water stress response, sulfate-deprived pea plants were subjected to a 9-days period of water stress during the early reproductive phase. While water stress did not impact seed yield, sulfur deficiency alone or combined wi…
Studying the interplay between sulfur nutrition and water stress tolerance in pea by proteomics : a focus on seed development and composition
2019
International audience; Water stress and sulfur-deficiency are two constraints increasingly faced by crops due to climatechange and low-input practices. To investigate their interplay in the grain legume pea (Pisum sativumL.), sulfate was depleted at mid-vegetative stage and a moderate 9-day water stress period was imposedduring the early reproductive phase. The combined stress accelerated seed production, lowering yield,one-seed weight and seed number per plant, but rebalanced seed protein composition. In fact, themoderate water stress mitigated the negative effect of sulfur-deficiency on the accumulation of sulfurrichproteins in seeds, probably due to a lower seed sink strength for nitrog…
Changes in the pea seed proteome in response to drought combined with sulfur deficiency
2017
EABAPGEAPSI DOCT INRA; Pea (Pisum sativum L.) produces seeds rich in proteins, but seed yield and quality remain unstable across years due to abiotic stresses occurring during the reproductive period. Drought and sulfur deficiency are two abiotic stresses that interact in the current context of climate change and lowinput practices, and recent studies suggest a role of sulfate transport and metabolism in the plant response to drought (Ernst et al., 2010; Chan et al., 2013; Gallardo et al., 2014; Ahmad et al., 2016). In this study, we investigated the impact of sulfur deficiency combined with drought on the pea seed proteome. Pea plants were subjected to sulfur-deficiency two weeks after sow…