Search results for "proteomics."
showing 10 items of 523 documents
Analysis of headspace of flavour compounds using PTR-ToF Mass Spectrometry: optimisation of acquisition parameters
2011
National audience
Cell imaging connected with protein mapping to investigate glyphosate induced toxicity on human keratinocytes
2009
During the skin ageing which is accelerated by environmental factors, the generated reactive oxygen species make unstable the "proliferation/differentiation/apoptosis" coupling of epidermal cells. A biochemical study, previously realized on human keratinocyte cultures treated by a pesticide, glyphosate, allowed the validation of an in vitro model of skin ageing. In this work, we propose to study the loss of HaCaT cell integrity appearing after this chemical induced-oxidative stress. An original approach, combining a micro- to nanoscale cell characterization and analysis of fluctuating protein expression, is proposed: a) the exploration of cell growth and morphology through fluorescence and …
'Shellome': Proteins involved in mollusk shell biomineralization - diversity, functions.
2011
18 pages; International audience
Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?
2007
International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…
Differential expression proteomics to investigate responses and resistance to Orobanche crenata in legumes
2007
International audience; In order to study the plant response to parasitic plants and the molecular basis of the resistance we have used a proteomic approach. The root proteome of two accessions of the model legume Medicago truncatula and crop pea displaying differences in their resistance phenotype, in control as well as in infected plants have been compared. We report quantitative as well as qualitative differences in the two-dimensional gel electrophoresis (2-DE) maps between plant genotypes and treatments (control, non-infected, and infected). Differential spots spots were successfully identified by peptide mass fingerprinting (PMF) following MALDI-TOF mass spectrometry. Many of the prot…
Differential protein expression in cytokine-sensitive and -resistant pancreatic beta cell lines
2009
Chemical Proteomics-Guided Identification of a Novel Biological Target of the Bioactive Neolignan Magnolol
2019
Understanding the recognition process between bioactive natural products and their specific cellular receptors is of key importance in the drug discovery process. In this outline, some potential targets of Magnolol, a natural bioactive compound, have been identified by proteomic approaches. Among them, Importin-β1 has been considered as the most relevant one. A direct binding between Magnolol and this nuclear chaperone has been confirmed by DARTS and molecular docking, while its influence on Importin-β1 translocation has been evaluated by in vitro assays.
Letter to the Editor: Solution structure of hypothetical protein TA1414 from Thermoplasma acidophilum
2004
Evaluation of cellular response of breast cancer cells grown on distinctive collagen substrates
2008
Breast cancer proteomics: from research to clinical applications.
2008
The exponentially increasing volume of information extracted from genomic and proteomic applications on cancer, while providing new insights into molecular composition of cancer cells and tissues, imposes new challenges on data rationalization as a tool for clinically relevant biomarker discovery. Breast cancer represents the most frequent and potentially aggressive type of cancer and, moreover, it is one of the most enigmatic and poorly predictable in its evolution, likely because it includes several different forms that behave differently among patients. Current clinical parameters for breast cancer diagnosis and cure are: tumour size, axillary lymph node status, histological grading and …