Search results for "pta"
showing 10 items of 4973 documents
Šūnā internalizējošas aptamēra-aptamēra konjugātsistēmas izveide
2020
Aptamēri ir oligonukleotīdi, kas savas trīsdimensionālās formas dēļ spēj saistīties ar specifiskiem mērķiem. Tie tiek plaši pētīti kā palīgs zāļu piegādāšanai vai slimību diagnostikā. Pēdējos gados padziļināti ir pētīta to pielietošana vēža terapijā. Bakalaura darba mērķis bija noskaidrot, aptamēra konstrukta AS1411 un Seq36 afinitāti uz gaišo nieru karcinomas šūnām. Lai pārliecinātos par AS1411 un Seq36 aptamēru konstrukta veiksmīgu izveidošanu, tika pielietota gela elektroforēze. Lai noteiktu cik funkcionāls un cik liela afinitāte ir uz gaišo nieru karcinomas šūnām, tika pielietota plūsmas citometrija. Pētījuma rezultāti liecina, ka aptamēru konstrukts parāda labāku saistīšanos ar gaišo n…
Selective downregulation of the MDR1 gene product in Caco-2 cells by stable transfection to prove its relevance in secretory drug transport.
2005
Considerable interest is focused on overcoming multidrug resistance (MDR) in cancer chemotherapy. The in vitro experiments to characterize P-glycoprotein's (P-gp) function and to decrease its effects have led to a variety of strategies such as addition of competitors or supplementation of the medium with oligonucleotides complementary to the 5'-end of the MDR1-mRNA. For the Caco-2 cell line, an in vitro model for absorption screening, expressing multiple transporters including P-gp, which pumps substances back into the apical solution, P-gp activity might mask other relevant transport proteins' activity. The objective of the present study was to construct a Caco-2 subline with reduced P-gp …
Review paper: Seasonal variation as a determinant of population structure in rotifers reproducing by cyclical parthenogenesis
1998
Monogonont rotifers live in habitats that display extensively variation in both biotic and abiotic components. Much of this variation is seasonal and therefore predictable for a given pond or lake. In 1972, King proposed one physiological and two genetic models presenting alternative modes of adaptation to this temporal variation. Our purpose in the present paper is to review and evaluate how our knowledge of the seasonal structure of rotifer populations has changed in the past 25 years. Seasonal changes in clone frequencies have been reported from three studies of natural populations using electrophoretic analysis of isozymes. In one of these studies there was evidence for substantial temp…
Un nuevo suido con dientes yugales tubulidentados e hipselorrizos del Mioceno inferior de Córcoles, España
2018
[EN] Excavations at Córcoles, Guadalajara, Spain (MN 4) in October 1987, yielded a mandible and several isolated teeth of a strange suiform with tubulidentate cheek teeth, initially interpreted to be the remains of Tubulidentata. The specimens are complete enough to remove any doubt about which Order (Artiodactyla) and Superfamily (Suoidea) they belong to. They are here attributed to the Family Doliochoeridae, being related to the genera Bransatochoerus (MP 30) and Lorancahyus (MN 2) both of which have hypsorhizic cheek teeth, the latter with tubules in the teeth, but not to the fully-expressed extent seen in the fossils from Córcoles and the molars retain a distinct and large pulp cavity, …
A Digital Twin for Lift Planning With Offshore Heave Compensated Cranes
2020
Abstract This paper presents a state-of-the-art digital twin of a hydraulic actuated winch that is used for heave compensation in offshore applications. The digital twin is used as part of a larger simulation model that involves all necessary components to perform lift planning and, subsequently, determine the corresponding weather window. The winch simulation model is described and verified by means of full-scale measurements. In addition, a set of acceptance criteria are presented that should be used whenever verifying digital twins of heave compensating winches that are to be used for lift planning.
Fostering Self-Compassion and Loving-Kindness in Patients With Borderline Personality Disorder: A Randomized Pilot Study.
2017
The aim of this randomized pilot study is to investigate the effects of a short training programme in loving-kindness and compassion meditation (LKM/CM) in patients with borderline personality disorder. Patients were allocated to LKM/CM or mindfulness continuation training (control group). Patients in the LKM/CM group showed greater changes in Acceptance compared with the control group. Remarkable changes in borderline symptomatology, self-criticism and self-kindness were also observed in the LKM/CM group. Mechanistic explanations and therapeutic implications of the findings are discussed. Highlights: Three weeks of loving-kindness and compassion meditations increased acceptance of the pres…
Mindfulness, acceptance and value-based intervention for burnout : mediational roles of learning the elements of the intervention on outcomes
2015
The effects of mindfulness-based interventions and ACT on mindfulness and well-being have received a lot of interest, and their mechanisms of change have been studied recently, but still some mechanisms are missing the research attention. The mechanisms of the change that happens during an intervention with people who suffer from work related burnout, have been examined by only a couple of studies. The present study aimed to investigate these mechanisms by looking at the mediating effects of learning mindfulness, acceptance and value-based working (MAV) skills which were measured with a novel Learning Questionnaire developed for the present research project. The data of the present study wa…
Characterizing smart tracking-based self-service technologies (STB SSTs) and their acceptance antecedents : a systematic literature review
2022
This paper introduces smart tracking-based self-service technologies (STB SSTs) as a new category of self-service. STB SSTs allow brand customers to voluntarily perform feedback-seeking that targets their brand-related consumption occurring in a connected environment, provided they consent to share private data with their brand. Despite an increasing deployment, this category of self-service is not well defined in the literature. In addition, we have little understanding of consumer motivations to use these technologies. The present work attempts to fill these gaps by performing an interpretive literature review. Our literaturereview highlights the necessity to distinguish between core and …
De l'opposabilité d'une clause attributive de juridiction insérée dans un contrat d'adhésion relatif au contrôle aérien
2010
International audience; Civ. 1re, 17 févr. 2010, n° 08-12.749, D. 2010. 588, obs. X. Delpech ; RTD. com. 2010. 460, obs. P. Delebecque
The value of accessibility in the culturalandcreative industries Translation-driven settings
2020
The accessibility of Cultural Creative Industries(CCI) has grown exponentially in different parts of the globe and has increasingly become part of projects of significant importance in contemporary societies that rely on informational, global, and networked systems of communication, i.e., developed countries. CCI exist within public-domain areas and are stimulated by the critical rethinking of the means which can be used to support cultural development. CCI are in fact driven by structures of the knowledge-based economy, where information, originality, and creativity contribute to providing stimuli to their rise. Their emergence is the result of advances in cultural spheres and movements, a…