Search results for "qPCR"
showing 10 items of 65 documents
Responses of soil bacterial and fungal communities to extreme soil drought and rewetting
2013
Question: The patterns of resource allocation and activity of the soil microbial community over the dry summer in Mediterranean grasslands are still largely unknown. The microbial response to summer desiccation reflects adaptation strategies, setting the stage for a large rainfall-induced soil CO2 pulse upon rewetting, an important component of the ecosystem carbon budget. Methods: In three California annual grasslands, the present (DNA-based) and potentially active (RNA-based) soil bacterial and fungal communities were tracked over a summer season and in response to controlled rewetting of intact soil cores. Phylogenetic marker genes for bacterial (16S) and fungal (28S) RNA and DNA were se…
Role of nitric oxide synthases from Klebsormidium nitens: structural characterization and identification of protein partners
2022
Nitric oxide (NO) is an important cellular signalling molecule regulating various physiological processes, in both animals andplants. In animals, NO synthesis is mainly catalysed by NO synthase (NOS) enzymes. During biotic or abiotic stresses, NOSlike activities that are sensitive to mammalian NOS inhibitors have been detected in plants, although no sequences encodingthe well described mammalian NOS are highlighted in land plants. Interestingly, we identified NOS-like sequences in 20 algaespecies. Among them, NOSs are found in Klebsormidium nitens the model alga to study the early transition step from aquaticalgae to land plants.As mechanisms governing NO synthesis and signalling in green l…
Differential responses of bacterial and archaeal groups at high taxonomical ranks to soil management
2010
Little is known about abundances of the major bacterial taxa in agricultural soils and how they are affected by fertilization or other agricultural practices. Our aim was to determine the abundance and relative distribution of several bacterial phyla and one class, as well as the archaeal and crenarchaeal communities, and how they were affected by different fertilization regimes to examine whether specific responses of microorganisms could be identified at these high taxonomic ranks. We used real-time PCR with taxa specific primers to quantify the abundance of the Actinobacteria, Acidobacteria, Bacteriodetes, Firmicutes, Gemmatimonadetes, Verrucomicrobia, Alphaproteobacteria and Crenarchaeo…
Impact of various factors on the diversity of soil microorganisms in agricultural and forest soils
2014
Elektroniskā versija nesatur pielikumus
Detection of Parietaria Mottle Virus by RT-qPCR: An Emerging Virus Native of Mediterranean Area That Undermine Tomato and Pepper Production in Southe…
2021
Parietaria mottle virus (PMoV) is considered an emerging virus in many countries of the Mediterranean basin, especially on tomato and pepper crops. Symptoms on tomato leaves and fruits can be easily confused with those induced by cucumber mosaic virus (CMV) with necrogenic satellite RNA (CMV-satRNA), tomato spotted wilt virus (TSWV) or tomato mosaic virus (ToMV). Mixed infection of these viruses has been also reported in some tomato cultivars, with an increase in the complexity of the symptoms and severity of the disease. Although a specific serum and riboprobes have been produced, nowadays no sensitive diagnostic methods are available for the rapid PMoV detection. Here, we have developed a…
Potencial uso de la tecnología basada en microARNs para el diagnóstico y pronóstico de la escoliosis idiopática del adolescente
2021
Introducción: La escoliosis idiopática del adolescente (EIA) es una enfermedad que se caracteriza por una deformación tridimensional de la columna vertebral cuya etiología es aún desconocida. Actualmente no existe una explicación clara sobre la evolución de la enfermedad; El diagnóstico y seguimiento actual de la EIA se basa en el análisis de la curvatura de la columna mediante radiografía de rayos X, la cual está considerada como la técnica gold standard. Sin embargo, esta técnica no tiene valor pronóstico y las repetidas exposiciones han demostrado incrementar la incidencia de cáncer en la edad adulta. Es necesario, por tanto, el desarrollo de una nueva herramienta para el diagnóstico tem…
Bioaugmentācijas izmantošana farmācijas notekūdeņu attīrīšanas efektivitātes uzlabošanai
2015
Šajā darbā tika pētīta no farmācijas attīrīšanas iekārtām izdalītu, dabisku mikroorganismu spēja degradēt specifiskus farmakoloģijas savienojumus tīrkultūru veidā, kā arī bioaugmentācijas procesā simulētos reālos vide apstākļos. Izmantotās metodes ļauj salīdzinoši novērtēt mikroorganismu koncentrāciju un dinamiku vidē. Tika parādīts, ka mikroorganismu aktivitāte testa sistēmas apstākļos ir būtiski atšķirīga no in vitro. Konstatēts, ka bioaugmentācijas celmiem ir augsta izskalošanas pakāpe un ir nepieciešams veikt papildus pasākumus, lai to samazinātu. Ir grūti paredzēt pievienoto mikroorganismu efektu uz eksistējošo mikrofloru. Sēnei Gibberella indica ir augsts potenciāls tikt izmantotai fa…
Development of molecular approaches for direct detection and quantification of wine-related microorganisms
2018
En esta tesis doctoral se han desarrollado procedimientos para la detección y cuantificación de levaduras y bacterias acéticas totales y, en concreto, de las especies más importantes en la vinificación como Saccharomyces cerevisiae, Brettanomyces bruxellensis, Zygosaccharomyces bailii, L. plantarum, y O. oeni. Las estrategias que se han utilizado para el desarrollo de nuevos métodos se centran fundamentalmente en la supresión del paso de extracción previa de ADN y de los procedimientos de eliminación de inhibidores de la reacción de PCR presentes en las muestras. Para llevar a cabo este objetivo general, se establecieron los siguientes objetivos específicos; 1, desarrollo de un método de qP…
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
2020
RNA interference (RNAi) is a powerful tool for studying functions of candidate genes in both model and non-model organisms and a promising technique for therapeutic applications. Successful application of this technique relies on the accuracy and reliability of methods used to quantify gene knockdown. With the limitation in the availability of antibodies for detecting proteins, quantitative PCR (qPCR) remains the preferred method for quantifying target gene knockdown after dsRNA treatment . We evaluated how qPCR primer binding site and target gene expression levels affect quantification of intact mRNA transcripts following dsRNA-mediated RNAi. The use of primer pairs targeting the mRNA sequ…
Subtelomēru rajona hromatīna struktūras izmaiņu noteikšana ar qPCR
2015
Cilvēka subtelomēri ir izteikti polimorfiski un ir ar vislielāko gēnu blīvumu visā genomā. Subtelomēru sekvences pārmaiņas ir saistītas ar mentālo atpalicību, ļaundabīgām hematoloģiskajām slimībām, atkārtotiem spontānajiem abortiem un citām slimībām. Tādēļ subtelomēru reģiona pārmaiņu konstatēšana ir svarīga klīniskās izpētes sastāvdaļa. Subtelomēru pētniecībā galvenokārt tiek pielietotas divas metodes: FISH un SAT. Šajā darbā tika aprakstīta qPCR metode subtelomēru analīzei, kas, teorētiski ir lētāka, ātrāka un precīzāka alternatīva FISH un SAT metodēm. Darbā apraksta qPCR metodes pielietošanu, Y hromosomas p pleca subtelomēru reģiona pārmaiņu pētīšanai, 34 DNS paraugiem, kas iegūti no nea…