Search results for "quantitative"
showing 10 items of 2409 documents
Increased serum miR-193a-5p during non-alcoholic fatty liver disease progression: Diagnostic and mechanistic relevance
2022
Background & Aims Serum microRNA (miRNA) levels are known to change in non-alcoholic fatty liver disease (NAFLD) and may serve as useful biomarkers. This study aimed to profile miRNAs comprehensively at all NAFLD stages. Methods We profiled 2,083 serum miRNAs in a discovery cohort (183 cases with NAFLD representing the complete NAFLD spectrum and 10 population controls). miRNA libraries generated by HTG EdgeSeq were sequenced by Illumina NextSeq. Selected serum miRNAs were profiled in 372 additional cases with NAFLD and 15 population controls by quantitative reverse transcriptase PCR. Results Levels of 275 miRNAs differed between cases and population controls. Fewer differences were seen wi…
A Multivariate Analysis of HIV-1 Protease Inhibitors and Resistance Induced by Mutation
2005
This paper describes the use of the multivariate statistical procedure principal component analysis as a tool to explore the inhibitory activity of classes of protease inhibitors (PIs) against HIV-1 viruses (wild type and more-frequent single mutants, V82A, V82F, and I84V) and against protease enzymes. The analysis of correlations between biological activity and molecular descriptors or similarity indexes allowed a reliable classification of the 51 derivatives considered in this study. The best results were obtained in the case of the I84V mutant for which a high number of predictions was achieved. On this basis, this statistical approach is proposed as a reliable method for the prediction …
Poly-sarcosine and poly(ethylene-glycol) interactions with proteins investigated using molecular dynamics simulations
2018
Nanoparticles coated with hydrophilic polymers often show a reduction in unspecific interactions with the biological environment, which improves their biocompatibility. The molecular determinants of this reduction are not very well understood yet, and their knowledge may help improving nanoparticle design. Here we address, using molecular dynamics simulations, the interactions of human serum albumin, the most abundant serum protein, with two promising hydrophilic polymers used for the coating of therapeutic nanoparticles, poly(ethylene-glycol) and poly-sarcosine. By simulating the protein immersed in a polymer-water mixture, we show that the two polymers have a very similar affinity for the…
Nonequilibrium thermodynamics of the RNA-RNA interaction underlying a genetic transposition program
2021
Thermodynamic descriptions are powerful tools to formally study complex gene expression programs evolved in living cells on the basis of macromolecular interactions. While transcriptional regulations are often modeled in the equilibrium, other interactions that occur in the cell follow a more complex pattern. Here, we adopt a nonequilibrium thermodynamic scheme to explain the RNA-RNA interaction underlying IS10 transposition. We determine the energy landscape associated with such an interaction at the base-pair resolution, and we present an original scaling law for expression prediction that depends on different free energies characterizing that landscape. Then, we show that massive experim…
Embedding Evolution in Epidemic-Style Forwarding
2007
International audience; In this work, we introduce a framework to let forwarding schemes evolve in order to adapt to changing and a priori unknown environments. The framework is inspired by genetic algorithms: at each node a genotype describes the forwarding scheme used, a selection process fosters the diffusion of the fittest genotypes in the system and new genotypes are created by combining existing ones or applying random changes. A case study implementation is presented and its performance evaluated via numerical simulations.
Integration of Gravitational Torques in Cerebellar Pathways Allows for the Dynamic Inverse Computation of Vertical Pointing Movements of a Robot Arm
2008
BackgroundSeveral authors suggested that gravitational forces are centrally represented in the brain for planning, control and sensorimotor predictions of movements. Furthermore, some studies proposed that the cerebellum computes the inverse dynamics (internal inverse model) whereas others suggested that it computes sensorimotor predictions (internal forward model).Methodology/principal findingsThis study proposes a model of cerebellar pathways deduced from both biological and physical constraints. The model learns the dynamic inverse computation of the effect of gravitational torques from its sensorimotor predictions without calculating an explicit inverse computation. By using supervised …
Optimization of cogging force in a linear permanent magnet generator for the conversion of sea waves energy
2015
In this paper an approach to the optimization of cogging force in a linear permanent magnet generator for the conversion of sea waves energy is presented. We have optimized the cogging force of a linear permanent magnet generator by using a 3D FEM parametric simulation. Several possible design solutions have been computed. A generator has been built and the results of the minimization procedure has been experimentally validated.
Quantitative Multispectral Imaging Differentiates Melanoma from Seborrheic Keratosis.
2021
Melanoma is a melanocytic tumor that is responsible for the most skin cancer-related deaths. By contrast, seborrheic keratosis (SK) is a very common benign lesion with a clinical picture that may resemble melanoma. We used a multispectral imaging device to distinguish these two entities, with the use of autofluorescence imaging with 405 nm and diffuse reflectance imaging with 525 and 660 narrow-band LED illumination. We analyzed intensity descriptors of the acquired images. These included ratios of intensity values of different channels, standard deviation and minimum/maximum values of intensity of the lesions. The pattern of the lesions was also assessed with the use of particle analysis. …
Extraction of ERP from EEG data
2007
In this article, a simple but novel technique for extracting a linear subspace related to event related potentials (ERPs) from ElectroEncephaloGraphy (EEG) data is introduced. The technique consists of a sequence of basic linear operations applied to multidimensional EEG data in a problem-specific manner. The derivation of the proposed technique is given and results with real data are described together with overall conclusions.
Magnetic resonance image segmentation and heart motion tracking with an active mesh based system
2002
International audience; Abstract: The work presented here relates to a method fir motion tracking in sequences of medical images. The purpose is to. quantify the general motions and the local deformations of a beating heart during a cardiac cycle. In order to achieve this goal, we first tessellate the,first image of the sequence into triangular patches. A Delaunay triangulation is applied to find the optimal set of triangles describing this image, giving a mesh covering the organs. One imposes the contours of the organs to correspond to edges of triangles so that each part of the heart (left ventricle, right ventricle, myocardium) can he described as a different set of triai izles, each set…