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showing 10 items of 4442 documents

Correlates of the non-use of contraception among female university students in Italy

2021

The present paper scrutinizes the correlates of the non-use of contraception among young women in Italy. To this end we consulted two releases of Selfy (Sexual and Emotional LiFe of Youths) survey, which offer information for a nationally sample of university students. The results reveal that the likelihood of non-using contraception is higher among women living in the South and among those with previous experience of unsafe sexual behaviours. The risk of unprotected sex is also higher within a group of students who are relatively older than the others and live in a stable cohabiting union.

ContraceptionLow fertilityItalyRisky behaviourUniversity studentSelfy SurveyContraception; University students; Risky behaviours; Low fertility; SELFY Survey; ItalyRisky behavioursSettore SECS-S/04 - DemografiaUniversity students
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Different modes of activating phosphofructokinase, a key regulatory enzyme of glycolysis, in working vertebrate muscle

2002

Glycolytic flux in white muscle can be increased several-hundredfold by exercise. Phosphofructokinase (PFK; EC 2.7.1.11) is a key, regulatory enzyme of glycolysis, but how its activity in muscle is controlled is not fully, understood. In order not to neglect integrative aspects of metabolic regulation, we have studied in frogs (Rana temporaria) a physiological form of muscle work (swimming) that can be triggered like a reflex. We analysed swimming to fatigue in well rested frogs, recovery from exercise, and repeated exercise after 2 h of recovery. At various times, gastrocnemius muscles were tested for glycolytic intermediates and effectors of PFK. All metabolites responded similarly to the…

medicine.medical_specialtyFructoseMetabolismBiologyBiochemistrychemistry.chemical_compoundEnzyme activatorEndocrinologychemistryInternal medicineExtracellularReflexmedicineMyocyteGlycolysisPhosphofructokinaseBiochemical Society Transactions
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Variation in the Senecio aethnensis group (Asteraceae) along an altitudinal gradient

2003

ABSTRACT The variability of the Senecio aethnensis group was investigated through morphometric analyses of seven populations along an altitudinal transect on Mt. Etna (E Sicily). The data obtained show a separation into two groups, one including the populations of the upper and middle belts, at and above 1700 m, the other including the populations from lower altitudes. In the first group a clinal morphological variation in several reproductive and vegetative characters was observed in relation to altitude. The analysis of leaf characters in progeny cultivated under uniform conditions confirmed the differentiation into the two groups, which are to be treated as separate species, i.e. S. aeth…

AltitudebiologySenecio aethnensisGroup (stratigraphy)BotanyMorphological analysisMorphological variationPlant ScienceAsteraceaeSeneciobiology.organism_classificationTransectEcology Evolution Behavior and SystematicsPlant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
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2019

Recent studies on extracellular RNA raised awareness that extracellular vesicles (EVs) isolated from cultured cells may co-purify RNAs derived from media supplements such as fetal bovine serum (FBS) confounding EV-associated RNA. Defined culture media supplemented with a range of nutrient components provide an alternative to FBS addition and allow EV-collection under full medium conditions avoiding starvation and cell stress during the collection period. However, the potential contribution of serum-free media supplements to EV-RNA contamination has remained elusive and has never been assessed. Here, we report that RNA isolated from EVs harvested from cells under serum-replacement conditions…

0301 basic medicineDifferential centrifugationHistologyChemistryRNACell BiologyMicrovesiclesReverse transcriptase03 medical and health sciencesChemically defined medium030104 developmental biology0302 clinical medicineBiochemistry030220 oncology & carcinogenesismicroRNAFetal bovine serumExtracellular RNAJournal of Extracellular Vesicles
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Tenascin in denervated human muscle

1996

Tenascin is a large oligomeric glycoprotein of the extracellular matrix. Its location is limited in innervated muscle tissues. We investigated immunohistologically, using two monoclonal antibodies (mab) against Tenascin, biopsied denervated human muscle of children and adults. Tenascin was present in the interstitial space among denervated muscle fibres. Accumulation of Tenascin in denervated adult muscle tissue was frequent, accumulation in denervated muscle tissue of children was sparse and weak. The two antibodies reacted correspondingly. Tenascin was not only found in the vicinity of atrophic muscle fibres, but also close to normally sized fibres, suggesting an early stage of denervatio…

chemistry.chemical_classificationMuscle tissueDenervationendocrine systemPathologymedicine.medical_specialtyanimal structuresbiologymedicine.drug_classTenascinmusculoskeletal systemMonoclonal antibodyExtracellular matrixmedicine.anatomical_structureNeurologychemistryInterstitial spaceembryonic structuresmedicinebiology.proteinImmunohistochemistryNeurology (clinical)GlycoproteinJournal of the Neurological Sciences
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Sex-specific interaction between arbuscular mycorrhizal and dark septate fungi in the dioecious plant Antennaria dioica (Asteraceae).

2012

Male and female plants of dioecious species often differ in their resource demands and this has been linked to secondary sexual dimorphism, including sex-specific interactions with other organisms such as herbivores and pollinators. However, little is known about the interaction between dioecious plants and fungal root endophytes. Plants may be simultaneously colonised by arbuscular mycorrhizal (AM) and dark septate (DS) fungi. While it is well established that AM mutualism involves reciprocal transfer of photosynthates and mineral nutrients between roots of host plants and these fungi, the role of DS fungi remains controversial. Here, we report the temporal and spatial variation in AM and …

Mutualism (biology)HerbivoreDioecyfungifood and beveragesAntennaria dioicaPlant ScienceGeneral MedicineBiologyAsteraceaeAsteraceaebiology.organism_classificationPlant RootsSexual dimorphismColonisationPollinatorMycorrhizaeBotanySymbiosisEcology Evolution Behavior and SystematicsFinlandSoil MicrobiologyPlant biology (Stuttgart, Germany)
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Low density lipoprotein receptor-related protein 1 mediated endocytosis of β1-integrin influences cell adhesion and cell migration.

2015

The low density lipoprotein receptor-related protein 1 (LRP1) has been shown to interact with β1-integrin and regulate its surface expression. LRP1 knock-out cells exhibit altered cytoskeleton organization and decreased cell migration. Here we demonstrate coupled endocytosis of LRP1 and β1-integrin and the involvement of the intracellular NPxY2 motif of LRP1 in this process. Mouse embryonic fibroblasts harboring a knock in replacement of the NPxY2 motif of LRP1 by a multiple alanine cassette (AAxA) showed elevated surface expression of β1-integrin and decreased β1-integrin internalization rates. As a consequence, cell spreading was altered and adhesion rates were increased in our cell model…

0301 basic medicineIntegrinBiologyFocal adhesion03 medical and health sciencesMiceCell MovementCell AdhesionAnimalsCell adhesionMice KnockoutCell adhesion moleculeIntegrin beta1Tumor Suppressor ProteinsCell migrationCell BiologyLRP1EndocytosisCell biologyMice Inbred C57BLDisease Models Animal030104 developmental biologyReceptors LDLbiology.proteinNeural cell adhesion moleculeIntracellularLow Density Lipoprotein Receptor-Related Protein-1Experimental cell research
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Cytosporones, coumarins, and an alkaloid from the endophytic fungus Pestalotiopsis sp. isolated from the Chinese mangrove plant Rhizophora mucronata

2009

Chemical examination of the endophytic fungus Pestalotiopsis sp., isolated from the leaves of the Chinese mangrove Rhizophora mucronata, yielded 11 new compounds including cytosporones J-N (1-3, 5-6), five new coumarins pestalasins A-E (8-12), and a new alkaloid named pestalotiopsoid A (14), along with the known compounds cytosporone C (4), dothiorelone B (7), and 3-hydroxymethyl-6,8-dimethoxycoumarin (13). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic data analysis.

Spectrometry Mass Electrospray IonizationMagnetic Resonance SpectroscopyClinical BiochemistryPharmaceutical ScienceBiochemistryEndophyteCell Linechemistry.chemical_compoundAlkaloidsAscomycotaCoumarinsDrug DiscoveryBotanyAnimalsHumansPestalotiopsisMolecular BiologyRhizophora mucronatabiologyAlkaloidOrganic ChemistryRhizophoraceaePhenolic acidFungi imperfectibiology.organism_classificationchemistryRhizophoraceaeMolecular MedicineSpectrophotometry UltravioletMangroveBioorganic & Medicinal Chemistry
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Isotope-dilution ICP-MS for trace element determination and speciation: from a reference method to a routine method?

2003

This critical review discusses the conditions under which inductively coupled plasma-isotope dilution mass spectrometry (ICP-IDMS) is suitable as a routine method for trace element and element-speciation analysis. It can, in general, be concluded that ICP-IDMS has high potential for routine analysis of trace elements if the accuracy of results is of predominant analytical importance. Hyphenated techniques with ICP-IDMS suffer both from lack of commercially available isotope-labeled spike compounds for species-specific isotope dilution and from the more complicated system set-up required for species-unspecific ICP-IDMS analysis. Coupling of gas or liquid chromatography with species-specific …

ChromatographyChemistryVaporizationExtraction (chemistry)Analytical chemistryTrace elementSample preparationIsotope dilutionMass spectrometryBiochemistryInductively coupled plasma mass spectrometryAnalytical ChemistryDilutionAnalytical and bioanalytical chemistry
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Traps N' Clots: NET-Mediated Thrombosis and Related Diseases.

2020

medicine.medical_specialtyHypertension PulmonaryArterial Occlusive DiseasesGastroenterologyExtracellular TrapsAutoimmune DiseasesTranslational Research BiomedicalMiceInternal medicineNeoplasmsThromboembolismmedicineAnimalsHumansInflammationVenous Thrombosisbusiness.industryThrombosisHematologymedicine.diseasePlatelet ActivationThrombosisSystemic Inflammatory Response SyndromeEndothelium VascularbusinessForecastingThrombosis and haemostasis
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