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showing 10 items of 4442 documents
Analytical Method for Quantification of Several Phthalate Acid Esters by Gas Chromatography-Mass Spectrometry in Coffee Brew Samples
2022
Several phthalate acid esters (PAEs), often called phthalate esters or phthalates, are substances classified as harmful due to their carcinogenic and mutagenic properties, and moreover, as dangerous for humans because they interfere with the endocrine system. In general, phthalic esters are used as plasticizers for different polymers and more other consumer products. In the present study, we describe a simple method to quantify PAEs in coffee brew using a liquid-liquid extraction without purification processes through analysing the obtained organic phase by GCMS in the single ion monitoring mode. The totals of single PAEs, in coffee brew samples analysed by us, are in the range of 159-5305 …
Práctica 12. Montaje con biseladora automática 3_A con tracer, plantilla o lentes demo, de unas lentes esfero-cilíndricas orgánicas y/o minerales, so…
2022
Montaje con biseladora automática 3_A con tracer, plantilla o lentes demo, de unas lentes esfero-cilíndricas orgánicas y/o minerales, sobre monturas de acetato y/o metal.
Práctica 13. Montaje con biseladora automática 3_B con tracer, plantilla o lentes demo, de unas lentes esfero-cilíndricas orgánicas y/o minerales, so…
2022
Montaje con biseladora automática 3_B con tracer, plantilla o lentes demo, de unas lentes esfero-cilíndricas orgánicas y/o minerales, sobre monturas de acetato y/o metal.
Kolorektālā audzēja šūnu līniju SW480 un SW620 ekstracelulāro vezikulu endocitozes ceļa noskaidrošana monocītu šūnu līnijā THP-1
2016
Vēža šūnu izdalītās ekstracelulārās vezikulas tiek uzskatītas par vienu no audzēju mikrovides starpšūnu komunikācijas līdzekļiem, kā arī metastātiskās nišas veidošanās iniciatoriem. Makrofāgi ir būtiska audzēja mikrovides sastāvdaļa, tāpēc darba mērķis bija noteikt audzēja šūnu izdalīto ekstracelulāro vezikulu endocitozes ceļu monocītu šūnu līnijā THP-1. Kolorektālā audzēja šūnu līniju SW480 un SW620 normoksijas un hipoksijas apstākļos izdalītās ekstracelulārās vezikulas iezīmēja ar fluorescento krāsvielu Syto RNASelect un noteica to uzņemšana THP-1 šūnās ar plūsmas citometrijas metodi. Izmantojot klatrīna, kaveolīna/lipīdu mikrodomēnu, fagocitozes un makropinocitozes inhibitorus, normoksij…
Processi tradizionali e polifenoli naturali per formaggi innovativi e nutraceutici
2018
l’aggiunta di composti antiossidanti naturali nei prodotti caseari ne aumenta il valore nutrizionale e il periodo di conservabilità
Huit objets de cuivre et de bronze du piémont pyrénéen oriental : analyse croisée typologique et métallique
2015
Cet article porte sur huit pièces à base de cuivre – deux haches plates et six poignards – provenant de la zone sous-pyrénéenne orientale (Corbières, Haute Vallée de l’Aude, Pays d’OImes), dans les départements de l’Aude et de l’Ariège de l’Est (région de Lavelanet). Elles sont décrites et replacées dans leur contexte typochronologique, alors que les aspects technoéconomiques sont envisagés via la composition métallique (composition élémentaire et rapports isotopiques du plomb). Cinq objets en cuivre – deux haches et trois poignards – sont attribués au Chalcolithique. L’une des haches se rapproche des types nord-ibériques et son importation depuis cette région est suggérée par les analyses …
Closed tank pneumatic press application to improve Sauvignon Blanc wine quality and nutraceutical properties
2019
The machines used in the winemaking process directly affect the quality of wine and its nutraceutical properties. Grapes-pressing is a very important step in winemaking as it may promote the presence and/or absence of enzymatic processes on the must, leading to the creation of different products in terms of chemical composition, starting from the same grapes. The aim of the study was to compare two different pressing systems of Sauvignon Blanc grapes using an innovative pneumatic discontinuous closed tank press in two operating modes: the traditional pressing mode in presence of oxygen and the inert pressing mode, performed through grapes pressing under inert gas with nitrogen recovery. Che…
Innovative and Applied Research in Biology: Proceedings, Vol. 2
2020
The collection contains SCIENTIFIC articles on the topics of the LU 78 conference reports. The main focus is on innovative and applied research in biology and interdisciplinary fields.
Exploration of extracellular vesicles from
2018
ABSTRACT The prevalent porcine helminth, Ascaris suum, compromises pig health and reduces farm productivity worldwide. The closely related human parasite, A. lumbricoides, infects more than 800 million people representing a disease burden of 1.31 million disability-adjusted life years. The infections are often chronic in nature, and the parasites have a profound ability to modulate their hosts’ immune responses. This study provides the first in-depth characterisation of extracellular vesicles (EVs) from different developmental stages and body parts of A. suum and proposes the role of these vesicles in the host–parasite interplay. The release of EVs from the third- (L3) and fourth-stage (L4)…
Aislamiento y caracterización de vesículas extracelulares en especies del género Candida
2017
Se ha desarrollado un método de obtención de exosomas, a partir de cultivos de levaduras del género Candida, que ha permitido obtener microvesículas de elevada homogeneidad y pureza. Mediante microscopía electrónica de transmisión y técnicas de detección de nanopartículas (NTA), se ha podido constatar la presencia de microvesículas de tamaños comprendidos entre 100 y 200 nm. La caracterización de proteínas llevada a cabo por técnicas de Western blot, ha puesto de manifiesto la elevada pureza de los exosomas obtenidos, no existiendo contaminación celular en las preparaciones, tal y como lo indican el resultado positivo frente a calnexina en el lisado celular y negativo en las vesículas extra…