Search results for "rase"
showing 10 items of 4343 documents
Dinamiskie un ģeoķīmiskie sālsūdens intrūzijas izcelsmes indikatori Akmens tilta pazemes ūdeņu novērojumu postenī
2018
Ūdens resursu kvalitāte ir viens no svarīgākajiem sociāl-ekonomiskajiem faktoriem pilsētu attīstībai. Piejūras lielo pilsētu pazemes ūdeņu krājumi ir pakļauti jūras intrūzijas riskam, ko izraisa pārmērīga pazemes ūdens ekspluatācija. Šāda situācija izveidojās Rīgā 20. gs. 70-tajos gados Gaujas ūdens horizontā, kas izraisīja pazemes ūdens līmeņa kritumu arī pārsedzošajos ūdens horizontos. Tiek uzskatīts, ka depresijas piltuves aizpildīšanās notiek dziļāk iegulošo ūdeņu augšupvērstas infiltrācijas rezultātā, tomēr trūkst detalizētu pētījumu, kas to pierādītu. Pētījuma mērķis ir noskaidrot sāļā ūdens izcelsmi Akmens tilta novērojumu postenī, izmantojot dinamiskos un ģeoķīmiskos ūdens izcelsmes…
‘How this holiday makes a difference’: The language of environment and the environment of nature in a cross-cultural study of ecotourism
2010
Partendo dal presupposto che le differenze culturali spesso costituiscono una fonte potenziale di problematiche traduttive, questo contributo mira a evidenziare le differenze che emergono sul piano linguistico, a livello lessicale, ma soprattutto fraseologico, in seguito a variegati orientamenti culturali che agiscono come filtri. Il linguaggio specialistico analizzato riguarda una nicchia particolare del mercato del turismo, ossia l’ecoturismo, che sta conquistando un terreno sempre più vasto in seguito ad una maggiore attenzione rivolta nei confronti di tematiche attuali, come quelle di impatto ambientale. Abbracciando una prospettiva interculturale, il lavoro indaga il diverso approccio …
Surfactant effect on the physicochemical characteristics of cationic solid lipid nanoparticles
2016
Solid lipid nanoparticles (SLNs) may be considered as a new approach for therapeutics for many diseases. In addition to drug delivery, their use as non-viral vectors for gene delivery can be obtained by including cationic lipids, which provide a positive surface potential that favors binding to the nucleic acids as DNA, siRNA, miRNA, etc. In fact, the addition of cationic surfactants is indispensable for obtaining nanoparticles with surface positive charge. In this study, three different cationic lipids (dioctadecyl dimethyl ammonium bromide, cetyltrimethyl ammonium bromide, cetylpyridinium chloride) and Brij 76 as nonionic surfactant were employed to formulate Precirol ATO 5 based cSLN usi…
Induction of apoptosis in human retinoblastoma cells by topoisomerase inhibitors
1998
PURPOSE:To examine the apoptotic effect induced in human retinoblastoma Y79 cells by camptothecin, etoposide, and amsacrine, to examine the effect of these drugs on the expression of many apoptosis-related modulators, and to test the antiapoptotic effect exerted by insulin-like growth factor-I (IGF-I). METHODS:Morphologic features of apoptosis were demonstrated using acridine orange- ethidium bromide staining and electron microscopy. DNA fragmentation was determined by means of an in situ cell detection procedure (TdT-dUTP terminal nick-end labeling [TUNEL]) or by electrophoresis on agarose gels and was quantified by enzyme-linked immunosorbent assay. The expression of apoptosis-related mod…
Common genetic denominators for Ca++-based skeleton in Metazoa: role of osteoclast-stimulating factor and of carbonic anhydrase in a calcareous spong…
2012
Calcium-based matrices serve predominantly as inorganic, hard skeletal systems in Metazoa from calcareous sponges [phylum Porifera; class Calcarea] to proto- and deuterostomian multicellular animals. The calcareous sponges form their skeletal elements, the spicules, from amorphous calcium carbonate (ACC). Treatment of spicules from Sycon raphanus with sodium hypochlorite (NaOCl) results in the disintegration of the ACC in those skeletal elements. Until now a distinct protein/enzyme involved in ACC metabolism could not been identified in those animals. We applied the technique of phage display combinatorial libraries to identify oligopeptides that bind to NaOCl-treated spicules: those oligop…
Microsporidia and Its Relation to Crohn's Disease. A Retrospective Study
2013
Background: The cause of Crohn's Disease (CD) remains unknown. Recently a decrease in the global lymphocyte population in the peripheral blood of CD patients has been reported. This decrease was more evident in gamma delta T lymphocytes, especially gamma delta CD8+T subsets. Furthermore, a decrease of IL-7 was also observed in these patients. We propose the hypothesis that microsporidia, an obligate intracellular opportunistic parasite recently related to fungi, in CD patients can take advantage of the lymphocytes and IL-7 deficits to proliferate and to contribute to the pathophysiology of this disease. Methods and Findings: In this case-control study, serum samples were collected from 36 C…
The Active Human Gut Microbiota Differs from the Total Microbiota
2011
The human gut microbiota is considered one of the most fascinating reservoirs of microbial diversity hosting between 400 to 1000 bacterial species distributed among nine phyla with Firmicutes, Bacteroidetes and Actinobacteria representing around of the diversity. One of the most intriguing issues relates to understanding which microbial groups are active players in the maintenance of the microbiota homeostasis. Here, we describe the diversity of active microbial fractions compared with the whole community from raw human fecal samples. We studied four healthy volunteers by 16S rDNA gene pyrosequencing. The fractions were obtained by cell sorting based on bacterial RNA concentration. Bacteria…
Hookworm-like eggs in children’s faecal samples from a rural area of Rwanda
2016
Background: Hookworm eggs identification and quantification is usually carried out by Kato-Katz method. However various structures present in the smear may be confused with eggs of such parasites. Objective: To document the presence of structures in Kato-Katz slides that could initially be misinterpreted as hookworm eggs. Method: 497 faecal samples were analysed by Kato-Katz technique, diphasic concentration technique, agar-plate coprocultive and larvae obtained were analysed by PCR and characterized by sequencing. Result: Hookworm-like eggs were found in 159 (32%) of the samples by Kato Katz, finally identified as Caenorhabditis elegans by PCR technique. Conclusion: The diagnosis of human …
Mitochondrial DNA content decreases during in vitro human embryo development: insights into mitochondrial DNA variation in preimplantation embryos do…
2020
Objective To assess the mitochondrial DNA (mtDNA) load and variation in human oocytes and during preimplantation embryo development using specimens donated for research. Design Prospective cohort study. Setting Not applicable. Patients A total of 50 in vitro fertilization patients and 11 oocyte donors whose specimens were obtained between July 2017 and July 2018. Interventions None. Main Outcome Measures All specimens were separately collected. Quantitative polymerase chain reaction was performed with SurePlex DNA Amplification System (Illumina). Primers for the adenosine triphosphate 8 mitochondrial gene and the β-actin were used. Data were statistically analyzed by analysis of variance wi…
The lipoprotein receptor LRP1 modulates sphingosine-1-phosphate signaling and is essential for vascular development
2014
Low density lipoprotein receptor-related protein 1 (LRP1) is indispensable for embryonic development. Comparing different genetically engineered mouse models, we found that expression of Lrp1 is essential in the embryo proper. Loss of LRP1 leads to lethal vascular defects with lack of proper investment with mural cells of both large and small vessels. We further demonstrate that LRP1 modulates Gi-dependent sphingosine-1-phosphate (S1P) signaling and integrates S1P and PDGF-BB signaling pathways, which are both crucial for mural cell recruitment, via its intracellular domain. Loss of LRP1 leads to a lack of S1P-dependent inhibition of RAC1 and loss of constraint of PDGF-BB-induced cell migra…