Search results for "reactors"
showing 10 items of 299 documents
Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defe…
2020
Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic…
Isolation of acetic, propionic and butyric acid-forming bacteria from biogas plants.
2015
In this study, acetic, propionic and butyric acid-forming bacteria were isolated from thermophilic and mesophilic biogas plants (BGP) located in Germany. The fermenters were fed with maize silage and cattle or swine manure. Furthermore, pressurized laboratory fermenters digesting maize silage were sampled. Enrichment cultures for the isolation of acid-forming bacteria were grown in minimal medium supplemented with one of the following carbon sources: Na(+)-dl-lactate, succinate, ethanol, glycerol, glucose or a mixture of amino acids. These substrates could be converted by the isolates to acetic, propionic or butyric acid. In total, 49 isolates were obtained, which belonged to the phyla Firm…
Recent advancements in lactic acid production - a review.
2017
Abstract Lactic acid, as an organic acid, has essential roles in industrial applications ranging from the food industry to life-sciences. Conventional fermentation methods have been well-studied since late 18th century, but are unable to achieve consumers' expectations regarding both quality and quantity. Therefore, novel technological developments of lactic acid production to increase yield and decrease over-all cost have become the primary goal. Genetic and metabolic engineering are great tools to overcome problems associated with product inhibition, undesired by-product formation, the negative effect of extreme culture conditions and most importantly inefficient use of expensive substrat…
Production of extracellular fructans by Gluconobacter nephelii P1464.
2015
UNLABELLED Bacterial extracellular fructans, known as levans, have potential applications in food, pharmaceutical and cosmetic industries and high fructan producing strains could contribute into the cost reduction and more extensive commercial usage of them. An acetic acid bacteria (AAB) isolate P1464 was obtained from the Microbial Strain Collection of Institute of Microbiology and Biotechnology, University of Latvia and identified as Gluconobacter nephelii by DNA-DNA hybridization and the formation of extracellular fructans by this strain was confirmed. Isolated extracellular fructose polymers were characterized using FT-IR spectroscopy and the structural features of fructan appeared as s…
Granulation and microbial community dynamics in the chitosan-supplemented anaerobic treatment of wastewater polluted with organic solvents.
2018
Abstract The effect of chitosan on the development of granular sludge in upflow anaerobic sludge blanket reactors (UASB) when treating wastewater polluted with the organic solvents ethanol, ethyl acetate, and 1-ethoxy-2-propanol was evaluated. Three UASB reactors were operated for 219 days at ambient temperature with an organic loading rate (OLR) of between 0.3 kg COD m −3 d −1 and 20 kg COD m −3 d −1 . One reactor was operated without the addition of chitosan, while the other two were operated with the addition of chitosan doses of 2.4 mg gVSS −1 two times. The three reactors were all able to treat the OLR tested with COD removal efficiencies greater than 90%. However, the time required to…
Nitrogen removal from on-site treated anaerobic effluents using intermittently aerated moving bed biofilm reactors at low temperatures
2005
Abstract On-site post-treatment of anaerobically pre-treated dairy parlour wastewater (DPWW e ; 10 °C) and mixture of kitchen waste and black water (BWKW e ; 20 °C) was studied in moving bed biofilm reactors (MBBR). The focus was on removal of nitrogen and of residual chemical oxygen demand (COD). Moreover, the effect of intermittent aeration and continuous vs. sequencing batch operation was studied. All MBBRs removed 50–60% of nitrogen and 40–70% of total COD (COD t ). Complete nitrification was achieved, but denitrification was restricted by lack of carbon. Nitrogen removal was achieved in a single reactor by applying intermittent aeration. Continuous and sequencing batch operation provid…
Biotrickling filter modeling for styrene abatement. Part 1: Model development, calibration and validation on an industrial scale
2017
Abstract A three-phase dynamic mathematical model based on mass balances describing the main processes in biotrickling filtration: convection, mass transfer, diffusion, and biodegradation was calibrated and validated for the simulation of an industrial styrene-degrading biotrickling filter. The model considered the key features of the industrial operation of biotrickling filters: variable conditions of loading and intermittent irrigation. These features were included in the model switching from the mathematical description of periods with and without irrigation. Model equations were based on the mass balances describing the main processes in biotrickling filtration: convection, mass transfe…
Aeration control in membrane bioreactor for sustainable environmental footprint
2020
In this study different scenarios were scrutinized to minimize the energy consumption of a membrane bioreactor system for wastewater treatment. Open-loop and closed-loop scenarios were investigated by two-step cascade control strategies based on dissolved oxygen, ammonia and nitrite concentrations. An integrated MBR model which includes also the greenhouse gas formation/emission processes was applied. A substantial energy consumption reduction was obtained for the closed-loop scenarios (32% for Scenario 1 and 82% for Scenario 2). The air flow control based on both ammonia and nitrite concentrations within the aerobic reactor (Scenario 2) provided excellent results in terms of reduction of o…
Removal of acetone from air emissions by biotrickling filters: providing solutions from laboratory to full-scale
2018
A full-scale biotrickling filter (BTF) treating acetone air emissions of wood-coating activities showed difficulties to achieve outlet concentrations lower than 125 mg C m-3, especially for high inlet concentrations and oscillating emissions. To solve this problem, a laboratory investigation on acetone removal was carried out simulating typical industrial conditions: discontinuous and variable inlet concentrations and intermittent spraying. The results were evaluated in terms of removal efficiency and outlet gas emission pattern. Industrial emissions and operational protocols were simulated: inlet load up to 70 g C m-3 h-1 during 2 cycles of 4 h per day and intermittent trickling of 15 min …
Anaerobic membrane bioreactor (AnMBR) scale-up from laboratory to pilot-scale for microalgae and primary sludge co-digestion: Biological and filtrati…
2020
This research work proposes the scale-up evaluation in terms of biological and filtration performance from laboratory to pilot-scale of an anaerobic membrane bioreactor (AnMBR) co-digesting raw microalgae and primary sludge. Best operating conditions for this scale-up were energetically and economically assessed based on laboratory results. Economic balance showed 3% higher annual costs when operating a reactor at 100 d solids retention time (SRT) compared to 70 d SRT. Energetic balance showed a 5.5-fold increase in heat demand working at thermophilic temperature comparing to mesophilic. The AnMBR operating conditions were set at 70 d SRT and 35 °C. The pilot-scale and lab-scale co-digester…