Search results for "reading"
showing 10 items of 1521 documents
Role of humanin, a mitochondrial-derived peptide, in cardiovascular disorders
2020
The mitochondria produce specific peptides-mitochondrial-derived peptides-that mediate the transcriptional stress response by their translocation into the nucleus and interaction with deoxyribonucleic acid. Mitochondrial-derived peptides are regulators of metabolism. This class of peptides comprises humanin, mitochondrial open reading frame of the 12S ribosomal ribonucleic acid type c (MOTS-c) and small humanin-like peptides (SHLPs). Humanin inhibits mitochondrial complex 1 activity and limits the level of oxidative stress in the cell. Data show that mitochondrial-derived peptides have a role in improving metabolic diseases, such as type 2 diabetes. Perhaps humanin can be used as a marker f…
RPGR ORF15 isoform co-localizes with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin
2005
The ORF15 isoform of RPGR (RPGR(ORF15)) and RPGR interacting protein 1 (RPGRIP1) are mutated in a variety of retinal dystrophies but their functions are poorly understood. Here, we show that in cultured mammalian cells both RPGR(ORF15) and RPGRIP1 localize to centrioles. These localizations are resistant to the microtubule destabilizing drug nocodazole and persist throughout the cell cycle. RPGR and RPGRIP1 also co-localize at basal bodies in cells with primary cilia. The C-terminal (C2) domain of RPGR(ORF15) (ORF15(C2)) is highly conserved across 13 mammalian species, suggesting that it is a functionally important domain. Using matrix-assisted laser desorption ionization time-of-flight mas…
RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets.
2019
Background MicroRNAs (miRNAs) are small non-coding RNA molecules mediating the translational repression and degradation of target mRNAs in the cell. Mature miRNAs are used as a template by the RNA-induced silencing complex (RISC) to recognize the complementary mRNAs to be regulated. To discern further RISC functions, we analyzed the activities of two RISC proteins, AGO2 and GW182, in the MCF-7 human breast cancer cell line. Methods We performed three RIP-Chip experiments using either anti-AGO2 or anti-GW182 antibodies and compiled a data set made up of the miRNA and mRNA expression profiles of three samples for each experiment. Specifically, we analyzed the input sample, the immunoprecipita…
Partial vinylphenol reductase purification and characterization from Brettanomyces bruxellensis
2008
International audience; Brettanomyces is the major microbial cause for wine spoilage worldwide and causes significant economic losses. The reasons are the production of ethylphenols that lead to an unpleasant taint described as 'phenolic odour'. Despite its economic importance, Brettanomyces has remained poorly studied at the metabolic level. The origin of the ethylphenol results from the conversion of vinylphenols in ethylphenol by Brettanomyces hydroxycinnamate decarboxylase. However, no information is available on the vinylphenol reductase responsible for the conversion of vinylphenols in ethylphenols. In this study, a vinylphenol reductase was partially purified from Brettanomyces bruxe…
Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracel…
2010
Abstract Background A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. Results One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resis…
Owning (and reading?) politeness books in the eighteenth century: an analysis through private libraries
2020
Este artículo explora la presencia en bibliotecas privadas españolas —y algunas americanas— del siglo XVIII de la literatura de urbanidad, considerada en un sentido amplio que incluye no solo manuales de civilidad propiamente dichos, sino también ensayos, periódicos, obras de educación, filosofía moral, medicina, ficción o viajes, en las que la reflexión sobre las costumbres y las pautas de vida civilizadas constituyó un eje importante. Aunque poseer libros no equivale a leerlos, el análisis de una veintena de inventarios y catálogos, complementados en ciertos casos con notas y recomendaciones de lectura, proporciona ciertos indicios sobre el lugar que la civilidad ocupó en las preocupacion…
Promoting Social and Political Change Through Pedagogy: Lorenzo Milani and the Barbiana School (Italy 1954–1967)
2015
This chapter describes the curriculum and pedagogy implemented at Barbiana, a small school in a remote and mountainous place in Italy. Still revered in Europe as one of the most interesting innovative teaching experiences, the school started in 1954 when Lorenzo Milani, a Catholic priest committed to work with the poor who had run an afterschool program in a working-class parish in an industrial city close to Florence, was sent to this location because of his leftist views. Milani believed that schools were class-based institutions that benefited bourgeois children and that excluded working-class and farmworker students. To reverse this situation, Milani contended that education for these s…
Applied Social Anthropology in the Researcher’s Own Society
2014
Professor Arne Martin Klausen (1927-) is the only social anthropologist in Norway who has tried to analyse the culture of the country as a whole. In doing this, he has explored several central themes of the country’s culture; like egalitarianism, the class journey, the strong tradition for development aid to poor countries, connected to a so-called humanitarian super-power which in its turn was an extension of Christian mission, the very wide-spread newspaper reading; however self-centered to national and local issues and finally, the collision between an elitist Olympic culture with Norwegian egalitarianism. Klausen also tried to tie some threads together in editing a collection of essays …
Producing social mobility. Class and Travel in the Romanian Novel 1901-1932
2020
The present article addresses the Romanian novelistic production between 1901 and 1932 in the attempt of identifying a series of patterns regarding the protagonists’ social mobility. Starting with the most mentioned destinations throughout the novels, I analyse how and why the different social classes travel and try to determine the landmarks between which they dispute their physical presence, on the one hand, and their aspirations, on the other. On this basis, the second part of the article conducts a quantitative analysis of the major means of transport in the period – the train, the tramway, the coach/carriage, the automobile, the aeroplane, the ship, and the waggon – and attempts to pin…
Teacher-perceived supportive classroom climate protects against detrimental impact of reading disability risk on peer rejection
2012
Abstract This study examined the role of a supportive classroom climate, class size, and length of teaching experience as protective factors against children's peer rejection. A total of 376 children were assessed in kindergarten for risk for reading disabilities (RD) and rated by their teachers on socially withdrawn and disruptive behaviors. The grade 1 measures included sociometric peer assessment and teachers' self-ratings of their supportiveness in the classroom, together with information on class size and teaching experience. The results showed, first, that the studied social and learning risk factors positively predicted peer rejection in grade 1. Moreover, teacher-reported supportive…