Search results for "real-time"
showing 10 items of 881 documents
An experimental testbed of an Internet of Underwater Things
2020
A number of critical features have so far slowed down the realization of an Internet of Underwater Things. The most relevant of these aspects are related to the unreliability of the communication channel, the long propagation delay and the effect of severe multi-path and fading. This paper presents the design and development of a hybrid underwater-terrestrial IoT where different underwater sensors collect heterogeneous data and use marine acoustic modems to send information to a gateway device; this is able to set up a long distance link, implemented through a LoRaWAN connection, to forward data to a remote cloud for further processing. Performance of this system has been tested in a real s…
Interaction of the retinoic acid signaling pathway with spicule formation in the marine sponge Suberites domuncula through activation of bone morphog…
2011
Abstract Background The formation of the spicules in siliceous sponges involves the formation of cylinder-like structures in the extraspicular space, composed of the enzyme silicatein and the calcium-dependent lectin. Scope of review Molecular cloning of the cDNAs (carotene dioxygenase, retinal dehydrogenase, and BMB-1 [bone morphogenic protein-1]) from the demosponge Suberites domuncula was performed. These tools were used to understand the retinoid metabolism in the animal by qRT-PCR, immunoblotting and TEM. Major conclusions We demonstrate that silintaphin-2, a silicatein-interacting protein, is processed from a longer-sized 15-kDa precursor to a truncated, shorter-sized 13 kDa calcium-b…
Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay.
1996
Summary Our aim was to evaluate the reverse transcription and polymerase chain reaction (RT/PCR) technique for the detection of rotavirus shedding by infected children as a routine diagnostic procedure, in comparison to the enzyme-linked immunosorbent assay (ELISA), electron microscopy (EM) and polyacrylamide gel etectrophoresis (PAGE) of rotavirus double stranded RNA. Two-hundred and twenty stool specimens were collected from infants and young children with diarrhoea, and 10–20% faecal suspensions were made. Several methods of rotavirus dsRNA extraction were assayed. Electrophoretic analysis of viral RNA was carried out on 10% polyacrylamide gols followed by silver staining. RT/PCR was per…
<title>Revenue-maximization-based adaptive WFQ</title>
2002
In the future Internet, di erent applications such as Voice over IP (VoIP) and Video-on-Demand (VoD) arise with di erent Quality of Service (QoS) parameters including e.g. guaranteed bandwidth, delay jitter, and latency. Different kinds of service classes (e.g. gold, silver, bronze) arise. The customers of di erent classes pay di erent prices to the service provider, who must share resources in a plausible way. In a router, packets are queued using a multi-queue system, where each queue corresponds to one service class. In this paper, an adaptive Weighted Fair Queue based algorithm for traAEc allocation is presented and studied. The weights in gradient type WFQ algorithm are adapted using r…
Endpoint Admission Control with Delay Variation Measurements for QoS in IP Networks.
2002
In this paper we describe a novel Endpoint Admission Control scheme (EAC) for IP telephony. EAC mechanisms are driven by independent measurements taken by the edge nodes on a flow of packets injected in the network to probe the source to destination path. Our scheme is characterized by two fundamental features. First, it does not rely on any additional procedure in internal network routers other than the capability to apply different service priorities to probing and data packets. Second, the connection admission decision is based on the analysis of the probing flow delay variation statistics. Simulation results, which focus on a IP telephony scenario, show that, despite the lack of core ro…
Adaptive live video streaming on low-cost wireless multihop networks for road traffic surveillance in smart cities
2021
Abstract Traffic surveillance is an important issue for Intelligent Transportation Systems (ITS) that helps detect incidents automatically, such as wrong-way drivers, still-standing vehicles and jams. Sometimes these systems require a fast and short-term deployment of video cameras. In these cases, ad-hoc networks could be a low-cost and feasible option, but they have poor performance for video delivery. Thus, we propose a smart live video adaptive streaming technique in order to transport video streams from the cameras to the external road traffic monitoring servers. To achieve this goal, these networks need a thorough study in order to analyze video quality under their inherent constraint…
An adaptive timer for RPL to handle mobility in wireless sensor networks
2016
This paper focuses on the performance of wireless sensor networks characterized by a hybrid topology composed of mobile and static sensor nodes. The Routing Protocol for Low power and lossy networks (RPL), which is standardized as an IPv6 routing protocol for low power and lossy networks, uses the trickle timer algorithm to handle changes in the network topology. However, this algorithm is not well adapted to dynamic environments. This paper enhances the trickle timer in order to fit with mobility requirements. Most of previous works have improved this algorithm without considering the random movement of nodes. In this work, the proposed timer algorithm takes into consideration the random t…
Increased serum miR-193a-5p during non-alcoholic fatty liver disease progression: Diagnostic and mechanistic relevance
2022
Background & Aims Serum microRNA (miRNA) levels are known to change in non-alcoholic fatty liver disease (NAFLD) and may serve as useful biomarkers. This study aimed to profile miRNAs comprehensively at all NAFLD stages. Methods We profiled 2,083 serum miRNAs in a discovery cohort (183 cases with NAFLD representing the complete NAFLD spectrum and 10 population controls). miRNA libraries generated by HTG EdgeSeq were sequenced by Illumina NextSeq. Selected serum miRNAs were profiled in 372 additional cases with NAFLD and 15 population controls by quantitative reverse transcriptase PCR. Results Levels of 275 miRNAs differed between cases and population controls. Fewer differences were seen wi…
PKM2 promotes Th17 cell differentiation and autoimmune inflammation by fine-tuning STAT3 activation
2019
Th17 cells undergo metabolic reprogramming towards glycolysis to support their differentiation and pathogenicity. Damasceno et al. report that PKM2, a glycolytic enzyme, plays a nonmetabolic role in mediating Th17 cell differentiation and autoimmune neuroinflammation by fine-tuning STAT3 activation.
Quantification of Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in non-spiked food products and evaluation of real-time PCR as…
2011
Abstract Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. are foodborne pathogens frequently associated with foods such as poultry, ready-to-eat products, fruits and vegetables. PCR-based procedures are rapid, sensitive and accurate; in particular, real-time PCR (qPCR), which besides being an automated high-throughput technique, allows quantification of foodborne pathogens. In the present work, qPCR-based methods were applied for the quantitative detection of E. coli O157:H7, Salmonella spp. and L. monocytogenes in a total of 306 non-spiked food samples in a study carried out in two laboratories simultaneously. qPCR allowed the detection of the three pathogens in around …