Search results for "real-time"
showing 10 items of 881 documents
Quantitative detection of viable foodborne E. coli O157:H7, Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazi…
2012
Abstract The increase of foodborne outbreaks associated with fresh vegetables has highlighted the importance of developing rapid and specific methods for the detection and quantification of foodborne pathogens. In this sense, real-time PCR (qPCR) fulfills these requirements although it may detect dead cells. Recently, a potential strategy to specifically detect viable cells has been proposed relying on the use of DNA binding molecules as sample pretreatment previous to the qPCR. In this study propidium monoazide (PMA) and reagent D, combined with qPCR, were evaluated for the detection and quantification of viable Escherichia coli O157:H7, Salmonella and Listeria monocytogenes. Initially, th…
APPLICATION OF REAL-TIME POLYMERASE CHAIN REACTION FOR RAPID DETERMINATION OFSALMONELLAIN RESTAURANT FOODS
2008
Several foods have been identified as frequently or occasionally contaminated with Salmonella, such as eggs, raw milk and all raw foods of animal origin that may carry salmonella and other bacteria. Salmonellosis is the most common food-borne illness, but it is usually preventable according to the implementation of a food safety system that includes Hazard Analysis and Critical Control Point. In this study, 96 food samples of the University restaurant of Valencia (Spain) were divided into four groups: first course; main course; accompaniment; and dessert, and were analyzed simultaneously with classical and fluorogenic polymerase chain reaction microbiological analysis. Neither of the proced…
A single method for recovery and concentration of enteric viruses and bacteria from fresh-cut vegetables
2011
Fresh-cut vegetables are prone to be contaminated with foodborne pathogens during growth, harvest, transport and further processing and handling. As most of these products are generally eaten raw or mildly treated, there is an increase in the number of outbreaks caused by viruses and bacteria associated with fresh vegetables. Foodborne pathogens are usually present at very low levels and have to be concentrated (i.e. viruses) or enriched (i.e. bacteria) to enhance their detection. With this aim, a rapid concentration method has been developed for the simultaneous recovery of hepatitis A virus (HAV), norovirus (NV), murine norovirus (MNV) as a surrogate for NV, Escherichia coli O157:H7, List…
Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells.
2015
The use of three-dimensional (3D) cultures may induce cardiac progenitor cells to synthesize their own extracellular matrix (ECM) and sarcomeric proteins to initiate cardiac differentiation. 3D cultures grown on synthetic scaffolds may favour the implantation and survival of stem cells for cell therapy when pharmacological therapies are not efficient in curing cardiovascular diseases and when organ transplantation remains the only treatment able to rescue the patient’s life. Silk fibroin-based scaffolds may be used to increase cell affinity to biomaterials and may be chemically modified to improve cell adhesion. In the present study, porous, partially orientated and electrospun nanometric n…
Industrial video sequences for network performance evaluation
2005
The analysis of video traffic characteristics plays a fundamental role in the evaluation of network performance or schedule design. However, the majority of these analyses are based on the study of traffic characteristics generated with different codifiers on typical general purpose films or sequences. These are generally made using asymmetric codifiers which are not usable in live real-time multimedia transmission systems. Moreover, the properties of these sequences are not necessarily equivalent to those obtained in an industrial environment. In this paper industrial monitoring video sequences are presented, describing their various characteristics, from the point of view of the source as…
A real-time webcam based Eye Ball Tracking System using MATLAB
2015
Eye Ball Tracking System is a device which is intended to assist patients that cannot perform any voluntary tasks related to daily life. Patients who only can control their eyes can still communicate with the real-world using the assistive devices like one proposed in this paper. This device provides a human computer interface in order to take decisions based on their eye movement. A real time data stream is captured via webcam that transfers data serially to MATLAB. Then a sequential image processing scheme segments the iris of the eye and calculates the centroid, thereby generating control signal with the help of a reference axis. The control signals are then used to manipulate the positi…
Developing a Tool Point Control Scheme for a Hydraulic Crane Using Interactive Real-time Dynamic Simulation
2010
This paper describes the implementation of an interactive real-time dynamic simulation model of a hydraulic crane. The user input to the model is given continuously via joystick and output is presented continuously in a 3D animation. Using this simulation model, a tool point control scheme is developed for the specific crane, considering the saturation phenomena of the system and practical implementation. This paper describes the implementation of an interactive real-time dynamic simulation model of a hydraulic crane. The user input to the model is given continuously via joystick and output is presented continuously in a 3D animation. Using this simulation model, a tool point control scheme…
Rejuvenative Efficacy and NF-κB Pathway-Dependent Mechanism of Human Platelet Lysate on Skin Aging
2021
Background: Skin aging is an irreversible process associated with poor self-regenerative capability. Human platelet lysate (hPL) contains rich amounts of growth factors, benefiting tissue regeneration. To explore the anti-aging efficacy and mechanism of hPL on skin, this study employed nude mice and human dermal fibroblasts (HDFs) to perform in vitro and in vivo assays. Methods: Flow cytometry and ELISA were conducted for quality control of hPL. In vivo, skin texture grading, histopathological observation, immunofluorescence analysis, and SOD and MDA assays were conducted to determine the effects of hPL on epidermis and dermis of aged skin. In vitro, senescence-associated-beta-galactosidase…
Age-associated telomere shortening in mouse oocytes
2013
Abstract Background Oocytes may undergo two types of aging. The first is induced by exposure to an aged ovarian microenvironment before being ovulated, known as ‘reproductive or maternal aging’, and the second by either a prolonged stay in the oviduct before fertilization or in vitro aging prior to insemination, known as ‘postovulatory aging’. However, the molecular mechanisms underlying these aging processes remain to be elucidated. As telomere shortening in cultured somatic cells triggers replicative senescence, telomere shortening in oocytes during reproductive and postovulatory aging may predict developmental competence. This study aimed to ascertain the mechanisms underlying altered te…
A Study of the Simulated Evolution of the Spectral Sensitivity of Visual Agent Receptors
2001
In this article we study a model for the evolution of the spectral sensitivity of visual receptors for agents in a continuous virtual environment. The model uses a genetic algorithm (GA) to evolve the agent sensors along with the control of the agents by requiring the agents to solve certain tasks in the simulation environment. The properties of the evolved sensors are analyzed for different scenarios. In particular, it is shown that the GA is able to find a balance between sensor costs and agent performance in such a way that the spectral sensor sensitivity reflects the emission spectrum of the target objects and that the capability of the sensors to evolve can help the agents significantl…