Search results for "report"
showing 10 items of 2364 documents
EGFP Reporters for Direct and Sensitive Detection of Mutagenic Bypass of DNA Lesions
2020
The sustainment of replication and transcription of damaged DNA is essential for cell survival under genotoxic stress
Cockayne syndrome: varied requirement of transcription-coupled nucleotide excision repair for the removal of three structurally different adducts fro…
2014
Hereditary defects in the transcription-coupled nucleotide excision repair (TC-NER) pathway of damaged DNA cause severe neurodegenerative disease Cockayne syndrome (CS), however the origin and chemical nature of the underlying DNA damage had remained unknown. To find out, to which degree the structural properties of DNA lesions determine the extent of transcription arrest in human CS cells, we performed quantitative host cell reactivation analyses of expression vectors containing various synthetic adducts. We found that a single 3-(deoxyguanosin-N 2-yl)-2-acetylaminofluorene adduct (dG(N 2)-AAF) constitutes an unsurmountable obstacle to transcription in both CS-A and CS-B cells and is remov…
Excision of Uracil from Transcribed DNA Negatively Affects Gene Expression
2014
Uracil is an unavoidable aberrant base in DNA, the repair of which takes place by a highly efficient base excision repair mechanism. The removal of uracil from the genome requires a succession of intermediate products, including an abasic site and a single strand break, before the original DNA structure can be reconstituted. These repair intermediates are harmful for DNA replication and also interfere with transcription under cell-free conditions. However, their relevance for cellular transcription has not been proved. Here we investigated the influence of uracil incorporated into a reporter vector on gene expression in human cells. The expression constructs contained a single uracil opposi…
Mutagenicity test system based on a reporter gene assay for short-term detection of mutagens (MutaGen assay).
2003
Abstract The construction of a bacterial mutation assay system detecting reversions of base substitutions and frameshifts in tetracycline (tet) and ampicillin resistance genes located on low copy plasmids is described. Frameshift mutations were introduced into repetitive GC-sequences and G-repeats known to be mutagenic hot-spots. Base pair substitutions were inserted in or around the active site of the ampicillinase gene thus generating reversibility of the ampicilline sensitivity. The plasmids carry genes to enable sensitive, fast and specific detection of mutagens in bacteria. MucAB was cloned into the test plasmid to enhance error-prone DNA-repair. The conventional reversion principle ha…
LrhA as a new transcriptional key regulator of flagella, motility and chemotaxis genes in Escherichia coli
2002
The function of the LysR-type regulator LrhA of Escherichia coli was defined by comparing whole-genome mRNA profiles from wild-type E. coli and an isogenic lrhA mutant on a DNA microarray. In the lrhA mutant, a large number (48) of genes involved in flagellation, motility and chemotaxis showed relative mRNA abundances increased by factors between 3 and 80. When a representative set of five flagellar, motility and chemotaxis genes was tested in lacZ reporter gene fusions, similar factors for derepression were found in the lrhA mutant. In gel retardation experiments, the LrhA protein bound specifically to flhD and lrhA promoter DNA (apparent K-D approximate to 20 nM), whereas the promoters of…
Las repercusiones de la crisis triguera de 1629-1631 sobre las finanzas de la ciudad de Valencia
2005
La incidencia de la crisis triguera de 1629-1631 obligó a la ciudad de Valencia a desarrollar diversos sistemas de avituallamiento. Pero ello no pudo impedir que se produjera un excepcional aumento de los precios del cereal, frente al cual el Municipio desarrolló una arriesgada política que abocó en la falta de numerario de la Taula de Canvis. La búsqueda de soluciones ante el déficit se tradujo en la elaboración, desde diferentes instancias políticas, de numerosos memoriales proponiendo múltiples y variados arbitrios frente a la crisis, cuyo análisis constituye el objetivo primordial de este artículo. The effect of the wheat crisis of 1629-1631 forced the city of Valencia to develop variou…
Inventando el crimen pasional: del lenguaje de gacetilla a la prensa de sucesos (España, 1892-1920)
2021
El terme «crim passional» va sorgir a la premsa espanyola el 1892. L’objectiu d’aquest treball és desvelar el perquè i entendre’n la relació amb la premsa sensacionalista i, en especial, amb la premsa especialitzada en successos. La nostra metodologia considera que la premsa és un motor cultural en la construcció de conceptes amb incidència social. Així, doncs, explorem la presència del terme «crim passional» en un bon nombre de diaris i setmanaris per observar com es va construir un marc mental de naturalesa emocional. Es revela la incidència del concepte en l’opinió pública i en el llenguatge jurídic, així com la seva rellevància en la justificació de la violència contra les dones.
Biases in study design, implementation, and data analysis that distort the appraisal of clinical benefit and ESMO-Magnitude of Clinical Benefit Scale…
2021
BACKGROUND: The European Society for Medical Oncology-Magnitude of Clinical Benefit Scale (ESMO-MCBS) is a validated, widely used tool developed to score the clinical benefit from cancer medicines reported in clinical trials. ESMO-MCBS scores assume valid research methodologies and quality trial implementation. Studies incorporating flawed design, implementation, or data analysis may generate outcomes that exaggerate true benefit and are not generalisable. Failure to either indicate or penalise studies with bias undermines the intention and diminishes the integrity of ESMO-MCBS scores. This review aimed to evaluate the adequacy of the ESMO-MCBS to address bias generated by flawed design, im…
Nationwide evaluation of day-to-day clinical pharmacists' interventions in German hospitals.
2015
tudy Objective To describe and evaluate the extent and diversity of nationwide data from clinical pharmacists’ interventions (PIs) in German hospitals. Design Retrospective analysis. Data Source The ADKA-DokuPIK German database, a national anonymous self-reported Internet-based documentation system for routine PIs as well as for medication errors reported by German hospital pharmacists. Measurements and Main Results Data sets from ADKA-DokuPIK entered between January 2009 and December 2012 were analyzed descriptively. A total of 27,610 PIs were entered, mainly by ward-based clinical pharmacists (82.5%). Most of the PIs were performed on surgical wards (37.8%), followed by anesthesiology/int…
The Upsides and Downsides of High Self-Control: Evidence for Effects of Similarity and Situation Dependency
2021
High trait self-control is generally depicted as favorable. We investigated whether this holds for social perception. Using vignettes, we tested whether a person with high self-control is 1) preferred as a partner for all or only certain social situations, 2) perceived as less likeable than a person with low self-control, 3) liked more if the person is female and the behavior thus fits the sex-stereotype, and 4) perceived differently from a person with low self-control with respect to a wide range of adjectives used to describe personality. Competing theories are presented for each area. Results indicate that although high self-control is associated with a wide range of socially desirable t…