Search results for "research article"

showing 10 items of 4621 documents

p24 Family Proteins Are Involved in Transport to the Plasma Membrane of GPI-Anchored Proteins in Plants

2020

p24 proteins are a family of type-I membrane proteins that cycle between the endoplasmic reticulum (ER) and the Golgi apparatus via Coat Protein I (COPI)- and COPII-coated vesicles. These proteins have been proposed to function as cargo receptors, but the identity of putative cargos in plants is still elusive. We previously generated an Arabidopsis (Arabidopsis thaliana) quadruple loss-of-function mutant affecting p24 genes from the δ-1 subclass of the p24 delta subfamily (p24δ3δ4δ5δ6 mutant). This mutant also had reduced protein levels of other p24 family proteins and was found to be sensitive to salt stress. Here, we used this mutant to test the possible involvement of p24 proteins in the…

0106 biological sciencesGenotypePhysiologyGlycosylphosphatidylinositolsMutantArabidopsisGolgi ApparatusPlant ScienceEndoplasmic Reticulum01 natural sciencessymbols.namesakeArabidopsisGeneticsArabidopsis thalianaResearch ArticlesbiologyChemistryArabidopsis ProteinsVesicleEndoplasmic reticulumCell MembraneGenetic VariationMembrane ProteinsCOPIGolgi apparatusbiology.organism_classificationCell biologyProtein TransportMembrane proteinMutationsymbols010606 plant biology & botany
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Frequency and density-dependent selection on life-history strategies--a field experiment.

2008

Negative frequency-dependence, which favors rare genotypes, promotes the maintenance of genetic variability and is of interest as a potential explanation for genetic differentiation. Density-dependent selection may also promote cyclic changes in frequencies of genotypes. Here we show evidence for both density-dependent and negative frequency-dependent selection on opposite life-history tactics (low or high reproductive effort, RE) in the bank vole (Myodes glareolus). Density-dependent selection was evident among the females with low RE, which were especially favored in low densities. Instead, both negative frequency-dependent and density-dependent selection were shown in females with high R…

0106 biological sciencesGenotypelcsh:MedicinePopulation geneticsEvolutionary Biology/Evolutionary EcologyBiology010603 evolutionary biology01 natural sciencesLife history theoryGene FrequencyGenetic variationEcology/Evolutionary EcologyHumansGenetic variabilitySelection Geneticlcsh:ScienceAllele frequencySelection (genetic algorithm)Population DensityMultidisciplinaryNatural selectionEvolutionary Biology/Animal Behaviorlcsh:RGenetic Variation010601 ecologyGenetics PopulationEcology/Population EcologyEvolutionary biologyPopulation cyclelcsh:QFemaleResearch ArticlePloS one
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Genomic variation in tomato, from wild ancestors to contemporary breeding accessions

2015

[EN] Background: Domestication modifies the genomic variation of species. Quantifying this variation provides insights into the domestication process, facilitates the management of resources used by breeders and germplasm centers, and enables the design of experiments to associate traits with genes. We described and analyzed the genetic diversity of 1,008 tomato accessions including Solanum lycopersicum var. lycopersicum (SLL), S. lycopersicum var. cerasiforme (SLC), and S. pimpinellifolium (SP) that were genotyped using 7,720 SNPs. Additionally, we explored the allelic frequency of six loci affecting fruit weight and shape to infer patterns of selection. Results: Our results revealed a pat…

0106 biological sciencesGermplasm[SDV.SA]Life Sciences [q-bio]/Agricultural sciencesBreeding01 natural sciencesNucleotide diversityDomesticationtomatefréquence des allèlespérouGene FrequencyCherry tomatoequateurSolanum lycopersicumexpression du génomeFruit size genes2. Zero hungerGenetics0303 health sciencesVegetal BiologyGenomebiologytaille du fruitfood and beveragesGenomicsSolanum lycopersicum;Solanum pimpinellifolium;SolCAP array;Origin;Variability;Genome;Fruit size genes;DomesticationSolanum pimpinellifoliumAgricultural sciencesSolCAP arrayGenome PlantResearch ArticleBiotechnologyHeterozygotePolymorphism Single NucleotideEvolution Molecular03 medical and health sciencesOriginGeneticsvariabilité génomique[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyAlleleGenomesVariabilityDomestication030304 developmental biologyGenetic diversityfungiSolanum pimpinellifoliumbiology.organism_classificationFruitaGENETICAFruitSolanumSciences agricolesBiologie végétale010606 plant biology & botanyamélioration des plantes
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Acting locally - affecting globally: RNA sequencing of gilthead sea bream with a mild Sparicotyle chrysophrii infection reveals effects on apoptosis,…

2019

[Background] Monogenean flatworms are the main fish ectoparasites inflicting serious economic losses in aquaculture. The polyopisthocotylean Sparicotyle chrysophrii parasitizes the gills of gilthead sea bream (GSB, Sparus aurata) causing anaemia, lamellae fusion and sloughing of epithelial cells, with the consequent hypoxia, emaciation, lethargy and mortality. Currently no preventive or curative measures against this disease exist and therefore information on the host-parasite interaction is crucial to find mitigation solutions for sparicotylosis. The knowledge about gene regulation in monogenean-host models mostly comes from freshwater monopysthocotyleans and almost nothing is known about …

0106 biological sciencesGillGillsApoptosis01 natural sciencesTranscriptomeSparus aurataGene expression0303 health sciencesHigh-Throughput Nucleotide Sequencingmedicine.anatomical_structureLiverHelminthiasis AnimalMonogeneaBiotechnologyResearch ArticleFish Proteinsanimal structureslcsh:QH426-470lcsh:BiotechnologyFisheriesSpleenBiologyMicrobiologyHost-Parasite Interactions03 medical and health sciencesImmune systemIllumina RNA-seqImmunitylcsh:TP248.13-248.65GeneticsmedicineAutophagyAnimals14. Life underwaterPlatelet activationImmune responseTranscriptomics030304 developmental biologyCell ProliferationSequence Analysis RNASparus aurata Sparicotyle chrysophrii Gills Monogenea Ectoparasites Illumina RNA-seq Transcriptomics Apoptosis Immune responseGene Expression ProfilingAquatic animalSea Breamlcsh:GeneticsGene Expression RegulationPlatyhelminthsSparicotyle chrysophriiEctoparasitesSpleen010606 plant biology & botany
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Harvest Pressure on Coastal Atlantic Cod (Gadus morhua) from Recreational Fishing Relative to Commercial Fishing Assessed from Tag-Recovery Data

2016

- Marine recreational fishing is a popular outdoor activity. However, knowledge about the magnitude of recreational catches relative to commercial catches in coastal fisheries is generally sparse. Coastal Atlantic cod (Gadus morhua) is a target species for recreational fishers in the North Atlantic. In Norway, recreational fishers are allowed to use a variety of traps and nets as well as long-line and rod and line when fishing for cod. From 2005 to 2013, 9729 cod (mean size: 40 cm, range: 15–93 cm) were tagged and released in coastal Skagerrak, southeast Norway. Both high-reward (NOK 500) and low-reward tags (NOK 50) were used in this study. Because some harvested fish (even those posting h…

0106 biological sciencesGillsRange (biology)Respiratory Systemlcsh:MedicineMarine and Aquatic Sciences01 natural sciencesVDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fangst: 925Commercial fishingGeographical LocationsRecreational fishingTheoretical EcologyMedicine and Health SciencesGadusMarine FishAnimal Anatomylcsh:ScienceMultidisciplinarybiologyEcologyEcologyNorwayFishesAgricultureHabitatsEuropeGeographyHabitatVertebratesAnatomyResearch ArticleDeath RatesFish BiologyFishingFisheriesMarine Biology010603 evolutionary biologyPopulation MetricsAnimalsRecreationDemographyPopulation Biology010604 marine biology & hydrobiologylcsh:REcology and Environmental SciencesOrganismsBiology and Life Sciencesbiology.organism_classificationFisheryAquatic Respiratory AnatomyPeople and PlacesEarth Scienceslcsh:QVDP::Agriculture and fisheries science: 900::Fisheries science: 920::Catch: 925Atlantic codZoologyPLoS ONE
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Antibiotics accelerate growth at the expense of immunity

2021

Antibiotics have long been used in the raising of animals for agricultural, industrial or laboratory use. The use of subtherapeutic doses in diets of terrestrial and aquatic animals to promote growth is common and highly debated. Despite their vast application in animal husbandry, knowledge about the mechanisms behind growth promotion is minimal, particularly at the molecular level. Evidence from evolutionary research shows that immunocompetence is resource-limited, and hence expected to trade off with other resource-demanding processes, such as growth. Here, we ask if accelerated growth caused by antibiotics can be explained by genome-wide trade-offs between growth and costly immunocompete…

0106 biological sciencesHOSTAntibioticsINSECTSDIVERSITYmicrobiomeMothsTrade-offkarjanhoito01 natural sciencesantibioticsimmunologiaINFECTIONgeeniekspressioLife History TraitsResearch ArticlesGeneral Environmental ScienceBODY-SIZE2. Zero hunger0303 health sciencesantibiootitTRADE-OFFSGeneral MedicineAnimal husbandryBiological EvolutionLEPIDOPTERAAccelerated GrowthAnti-Bacterial AgentsWARNING COLORATIONimmuunijärjestelmäLarvaeläimetBACTERIAImmunocompetenceGeneral Agricultural and Biological SciencesRNA-interferenssimedicine.drug_classjalostusZoologykotieläintuotantoBiology010603 evolutionary biologyGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesImmune systemgrowth promotionImmunityPARASEMIAmedicineAnimalsMicrobiomeimmunity trade-off1172 Environmental sciences030304 developmental biologygeenitGeneral Immunology and MicrobiologyGenetics and Genomicsmikrobisto3111 Biomedicine
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Genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly Bemisia tabaci

2015

Background The whitefly Bemisia tabaci is an important agricultural pest with global distribution. This phloem-sap feeder harbors a primary symbiont, “Candidatus Portiera aleyrodidarum”, which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. Interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbionts. Results In this paper, we analyzed the genome sequences of the primary symbiont Portiera and of the secondary symbiont Hamiltonella in the B. tabaci Mediterranean (MED) species in orde…

0106 biological sciencesHamiltonellaCandidatus Portiera aleyrodidarum[SDV]Life Sciences [q-bio]Molecular Sequence DataWhiteflyPortiera010603 evolutionary biology01 natural sciencesGenomeHemiptera03 medical and health sciencesMetabolic complementationSymbiosisEnterobacteriaceaeBotanyGeneticsAnimalsAmino AcidsSymbiosisIn Situ Hybridization Fluorescence030304 developmental biology2. Zero hungerGenetics0303 health sciencesEndosymbiontGenomebiologyfungifood and beveragesHigh-Throughput Nucleotide SequencingDNASequence Analysis DNAVitaminsbiochemical phenomena metabolism and nutritionbiology.organism_classificationEnterobacteriaceaeHemipteraWhiteflyComplementationHalomonadaceaeGlobal distribution[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]Genome BacterialMetabolic Networks and PathwaysBiotechnologyResearch ArticleBMC Genomics
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Improving the conservation of Mediterranean Chondrichthyans : the ELASMOMED DNA barcode reference library

2017

Cartilaginous fish are particularly vulnerable to anthropogenic stressors and environmental change because of their K-selected reproductive strategy. Accurate data from scientific surveys and landings are essential to assess conservation status and to develop robust protection and management plans. Currently available data are often incomplete or incorrect as a result of inaccurate species identifications, due to a high level of morphological stasis, especially among closely related taxa. Moreover, several diagnostic characters clearly visible in adult specimens are less evident in juveniles. Here we present results generated by the ELASMOMED Consortium, a regional network aiming to sample …

0106 biological sciencesHeredityMolecular biologySpeciationBiodiversitySettore BIO/05 - Zoologialcsh:MedicineJuvenileEvolutionary biologyBarcodeBiochemistry01 natural sciencesDNA barcodinglaw.inventionlawDNA librarieslcsh:ScienceChondrichthyesSpecimens IdentificationData ManagementMolecular systematicsMultidisciplinaryGeographyMediterranean RegionEcologyCryptic speciationFishesBiodiversityMorphological stasiMitochondrial DNANucleic acidsGenetic MappingPhylogeographyBiogeographyDNA Barcode Reference LibraryDNA barcoding TaxonomicVertebratesDNA data banksResearch ArticleComputer and Information SciencesConservation of Natural ResourcesEvolutionary ProcessesEvolutionary systematicsBarcoding Chondrichthyans Conservation Mediterranean Sea mtDNACartilaginous fishConservationBiologyPhylogeographic structure010603 evolutionary biologyMolecular taxonomySpecies SpecificityEndemic SpeciesGeneticsMediterranean SeaAnimalsDNA Barcoding TaxonomicDNA barcoding14. Life underwaterEndemismTaxonomyPopulation Biology010604 marine biology & hydrobiologylcsh:REcology and Environmental SciencesOrganismsBiology and Life SciencesDNAResearch and analysis methodsPhylogeographyMolecular biology techniquesTaxonHaplotypesThreatened speciesEarth SciencesSharksConservation statuslcsh:QPopulation GeneticsMarine biodiversity conservationElasmobranchii
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Variation in Hsp70 Levels after Cold Shock: Signs of Evolutionary Responses to Thermal Selection among Leptinotarsa decemlineata Populations

2012

Individuals of widely spread species are expected to show local adaption in temperature tolerance as they encounter a range of thermal conditions. We tracked thermal adaptations of the Colorado potato beetle (Leptinotarsa decemlineata) that invaded Europe within the last 100 years. It has occupied various conditions although, like the majority of invasive species, it lost a measurable amount of neutral genetic variation due to bottleneck effect when it invaded Europe. We exposed diapausing beetles originated from three different latitudes (54°N, 59°N, 60°N) to cold shock (−5°C, 1.5 hrs) in order to test if beetles from the northern populations express differential levels of cold-induced and…

0106 biological sciencesHeredityRange (biology)AcclimatizationPopulationlcsh:MedicineIntroduced speciesDiapause010603 evolutionary biology01 natural sciencesInvasive species03 medical and health sciencesGeneticsAnimalsHspHSP70 Heat-Shock Proteinslcsh:ScienceeducationBiologyLeptinotarsa030304 developmental biologyEvolutionary Biology0303 health scienceseducation.field_of_studyMultidisciplinaryEcologyPopulation BiologybiologyEcologylcsh:RColorado potato beetlekoloradonkuoriaineninvaasio15. Life on landbiology.organism_classificationAdaptation PhysiologicalBiological EvolutionCold TemperatureColeoptera13. Climate actionta1181lcsh:QlämpötilaAdaptationPopulation GeneticsResearch ArticlePLoS ONE
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Convergences and divergences between scientific and Indigenous and Local Knowledge contribute to inform carnivore conservation

2021

There is increasing recognition that diverse knowledge systems can work in mutually enriching ways and that Indigenous and Local Knowledge (ILK) can enhance biodiversity conservation. However, studies using scientific knowledge and ILK in a complementary manner, and acknowledging convergent and especially divergent insights have remained limited. In this study, we contrasted proxies of abundances and trends of threatened and conflict-prone carnivores (caracal, cheetah, jackal, lion, leopard, spotted hyaena, striped hyaena) derived separately from scientific knowledge and ILK. We conducted camera trapping, track surveys and semi-structured interviews with local pastoralists from northern Ken…

0106 biological sciencesIndigenous and Local KnowledgeSociology of scientific knowledgeScientific knowledgeGeography Planning and DevelopmentCarnivorapaikallisyhteisöt01 natural sciencesindigenous and local knowledgetieteellinen tietopetokannatKeniascientific knowledgeEcologybiologylajiensuojeluLeopardGeneral MedicineBiodiversity010601 ecologyGeographyCarnivore conservationHyaenaJackal1181 Ecology evolutionary biologyluonnonsuojeluResearch ArticlekokemustietoConservation of Natural ResourcesCamera trappingEcology (disciplines)010603 evolutionary biologyIndigenousKnowledge-based systemsbiology.animalEnvironmental ChemistryAnimalsHumansPantherasuurpedotcarnivore conservationEnvironmental planning1172 Environmental sciencestrack survey15. Life on landbiology.organism_classificationKenyaTrack surveycamera trappingeläinten jäljetThreatened species
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