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showing 10 items of 2426 documents
MACVIA-ARIA Sentinel NetworK for allergic rhinitis (MASK-rhinitis): the new generation guideline implementation
2015
Accepted Article
Nasal Cellularity in 183 Unselected Schoolchildren Aged 9 to 11 Years
2002
Objective. Although rhinitis is extremely frequent in children, methods for assessing the severity of nasal inflammation produce results with wide variability and hence weak clinical significance. We designed this epidemiologic investigation to define the clinical usefulness of assessing nasal cellularity in children. Methods. We studied 183 of 203 eligible unselected schoolchildren who were aged 9 to 11 years and whose parents gave informed consent and completed a questionnaire on the history of atopic and respiratory symptoms. In all children, nasal swabs were obtained from both nostrils and eluted in saline and slides were prepared from cytospin preparations for staining and white cell c…
Determination of muscimol and ibotenic acid in mushrooms of Amanitaceae by capillary electrophoresis
2014
In this study, the CZE method for rapid quantitative and qualitative determination of ibotenic acid and muscimol in Amanita mushrooms naturally grown in Poland was developed. The investigations included the species of A. muscaria, A. pantherina, and A. citrina, collected in southern region of Poland. The studied hallucinogenic compounds were effectively extracted with a mixture of methanol and 1 mM sodium phosphate buffer at pH 3 (1:1 v/v) using ultrasound-assisted procedure. The obtained extracts were separated and determined by CZE utilizing a 25 mM sodium phosphate running buffer adjusted to pH 3 with 5% content of acetonitrile v/v. The calibration curves for both analytes were linear in…
Dissociation between priming and recognition in the expression of sequential knowledge
2002
Exposure to a repeating sequence of target stimuli in a speeded localization task can support both priming of sequence-consistent responses and recognition of sequence components. Here, a task is introduced in which measures of priming and recognition are obtained concurrently, and it is demonstrated that priming of sequence-consistent responses occurs even when test stimuli are not recognized. The results show that sequence knowledge can be expressed in the absence of conscious recognition. However, we also show that this result is consistent with a simple model in which priming and recognition depend on exactly the same underlying memory strength variable.
Statistical methods for the comparison of measurements derived from orthodontic imaging
2000
Biometric comparison procedures for dental imaging methods with continuous outcome were reviewed, mainly concentrating on assessment and comparison of accuracy and precision according to the study design. Univariate graphical and numerical representations of corresponding deviations were summarized to derive a 'check list' of minimum information necessary to compare the measurement methods. The methods reviewed in this investigation are illustrated by the comparison of conventional (radiographic) cephalometry versus assessment using the DigiGraph in 50 female children. A paired t-test and the corresponding confidence interval approach were used to assess deviations in location of two imagin…
Selective determination of trimethylamine in air by liquid chromatography using solid phase extraction cartridges for sampling.
2004
Abstract The selective determination of trimethylamine (TMA) in air by liquid chromatography is reported. Sampling is effected by flushing air through C18-packed solid-phase extraction (SPE) cartridges at a flow rate of 15 mL/min for 15 min. Next, TMA is desorbed from the cartridges and injected into the chromatographic system. The analyte is then selectively retained on a precolumn ( 20 mm ×2.1 mm i.d., packed with 30 μm, Hypersil C18 phase), and derivatized on-line by injecting 9-fluorenylmethyl chloroformate (FMOC). Finally, the TMA-FMOC derivative is transferred to the analytical column ( 125 mm ×4 mm i.d., LiChrospher 100 RP18, 5 μm), and monitored at 262 nm. The method was applied to …
Application of solid-phase microextraction combined with derivatization to the determination of amphetamines by liquid chromatography
2004
This work evaluates the utility of solid-phase microextraction (SPME) in the analysis of amphetamines by liquid chromatography (LC) after chemical derivatization of the analytes. Two approaches have been tested and compared, SPME followed by on-fiber derivatization of the extracted amphetamines, and solution derivatization followed by SPME of the derivatives formed. Both methods have been applied to measure amphetamine (AP), methamphetamine (MA), and 3,4-methylenedioxymethamphetamine (MDMA), using the fluorogenic reagent 9-fluorenylmethyl chloroformate (FMOC) and carbowax-templated resin (CW-TR)-coated fibers. Data on the application of the proposed methods for the analysis of different kin…
Sub- and supercritical fluid extraction of trichloropyridinol from soil prior to immunoassay
1997
A comparative study on the extraction of TCP (3,5,6-trichloro-2-pyridinol, a metabolite of chlorpyrifos) from soil with CO2 and H2O is reported. The polarity of the analyte requires the presence of both a cosolvent (methanol) and an ion-pair reagent [(1R)-(-)-10-camphorsulfonic acid ammonium salt] for 95% extraction in 30 min when supercritical CO2 at 40 degrees C and 383 bar is used as extractant. Subcritical water (250 degrees C and 200 bar) enables complete extraction within 15 min without additives. Quantitation of the target analyte is performed by specific immunoassay using a non-commercial monoclonal antibody which provides a linear determination range between 0.005 and 5 micrograms/…
Validation of a confirmatory method for the determination of macrolides in liver and kidney animal tissues in accordance with the European Union regu…
2006
Abstract This study proposes a simple multiresidue liquid chromatography–diode array detector (LC–DAD) method capable of determining seven macrolide antibiotics in samples of liver and kidney animals at concentrations lower than those allowed by current legislation. Samples were prepared by homogenizing the tissue with EDTA–McIlvaine's buffer and extracted with an Oasis HLB cartridge. The consumption of organic solvent during extraction was minimum. The analytes were detected by LC–DAD and also by liquid chromatography–mass spectrometry with electrospray ionization (LC–(ESI)MS). The method was specific, stable and robust enough for the required purposes. The DAD method was validated in acco…
Development and validation of a fast and sensitive chromatographic assay for all-trans-retinol and tocopherols in human serum and plasma using liquid…
2002
A sensitive HPLC assay for all-trans-retinol, alpha-tocopherol, and gamma-tocopherols in human serum and plasma is reported. Sample preparation is performed in one step and involves precipitation of proteins and extraction of lipids with two volumes of an ethanol-chloroform mixture (3:1, v/v) without I.S. addition. After removal of the precipitated protein, 20 microl aliquots of the supernatant (equivalent to 6.7 microl of serum or plasma) were injected into the HPLC system and analyzed using fluorometric detection. RP-HPLC was performed using a C(18) S3 ODS2 column with a methanol-water step gradient (97:3 to 100) at 1.0 ml/min. The quantification limit expressed as nanograms of analyte pe…