6533b7d6fe1ef96bd1265db8
RESEARCH PRODUCT
Sub- and supercritical fluid extraction of trichloropyridinol from soil prior to immunoassay
J.j. ManclúsM.m Jiménez-carmonaA. MontoyaM.d.luque De Castrosubject
AnalyteChromatography GasHot TemperatureChromatographymedicine.diagnostic_testPyridonesOrganic ChemistryExtraction (chemistry)Supercritical fluid extractionReproducibility of ResultsEnzyme-Linked Immunosorbent AssayGeneral MedicineBiochemistrySupercritical fluidAnalytical Chemistrychemistry.chemical_compoundchemistryImmunoassayReagentPressuremedicineSoil PollutantsAmmoniumMethanoldescription
A comparative study on the extraction of TCP (3,5,6-trichloro-2-pyridinol, a metabolite of chlorpyrifos) from soil with CO2 and H2O is reported. The polarity of the analyte requires the presence of both a cosolvent (methanol) and an ion-pair reagent [(1R)-(-)-10-camphorsulfonic acid ammonium salt] for 95% extraction in 30 min when supercritical CO2 at 40 degrees C and 383 bar is used as extractant. Subcritical water (250 degrees C and 200 bar) enables complete extraction within 15 min without additives. Quantitation of the target analyte is performed by specific immunoassay using a non-commercial monoclonal antibody which provides a linear determination range between 0.005 and 5 micrograms/g, with coefficients of variation of 5.3 and 4.9% for the SC-CO2 and sub-H2O extractions, respectively.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-10-01 | Journal of Chromatography A |