Search results for "rpoB"

2014

A pure culture of an actinobacterium previously described as ‘Candidatus Rhodoluna lacicola ’ strain MWH-Ta8 was established and deposited in two public culture collections. Strain MWH-Ta8T represents a free-living planktonic freshwater bacterium obtained from hypertrophic Meiliang Bay, Lake Taihu, PR China. The strain was characterized by phylogenetic and taxonomic investigations, as well as by determination of its complete genome sequence. Strain MWH-Ta8T is noticeable due to its unusually low values of cell size (0.05 µm3), genome size (1.43 Mbp), and DNA G+C content (51.5 mol%). Phylogenetic analyses based on 16S rRNA gene and RpoB sequences suggested that strain MWH-Ta8T is affiliated …

Molecular epidemiology and whole genome sequencing analysis of clinical Mycobacterium bovis from Ghana

2019

[Background]: Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a re-emerging problem in both livestock and humans. The association of some M. bovis strains with hyper-virulence, MDR-TB and disseminated disease makes it imperative to understand the biology of the pathogen.

Mutations in the rpoB and katG Genes Leading to Drug Resistance in Mycobacterium tuberculosis in Latvia

2002

ABSTRACT To characterize the genetic basis of drug resistance in Mycobacterium tuberculosis in Latvia, mutations involved in rifampin ( rpoB gene) and isoniazid ( katG gene) resistance in DNA from 19 drug-susceptible and 51 multidrug-resistant M. tuberculosis complex isolates were analyzed. The most frequent rpoB gene mutations found by the Line Probe assay were the S531L (14 of 34 isolates), D516V (7 of 34), H526D (4 of 34), and D516Y plus P535S (4 of 34) mutations. Direct sequencing of seven isolates with unclear results from Line Probe assay showed the presence of the L533P mutation and the Q510H plus H526Y (1 of 34) and D516V plus P535S (4 of 34) double mutations, neither of which has b…

Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov

2019

AbstractKlebsiella oxytocacauses opportunistic human infections and post-antibiotic haemorrhagic diarrhoea. ThisEnterobacteriaceaespecies is genetically heterogeneous and is currently subdivided into seven phylogroups (Ko1 to Ko4, Ko6 to Ko8). Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well-defined sequence clusters related to, but distinct from,Klebsiella michiganensis(Ko1),Klebsiella oxytoca(Ko2),K. huaxiensis(Ko8) andK. grimontii(Ko6). The average nucleotide identity of Ko3 and Ko4 were 90.7% withK. huaxiensisand 95.5% withK. grimontii, respectively. In addition, three strains ofK.…

Genotyping of the genus Proteus by rpoB sequence analysis

2005

Background. The genetic relationship among different species within the genus Proteus has still not been clarified and previous studies by rRNA gene restriction patterns (ribotypes) suggested a high genetic variability in the presently recognised nomenspecies. Methods. The usefulness of rpoB sequencing for inter- and intraspecies discrimination in the genus Proteus was evaluated on 11 type and clinical strains belonging to the four described species: P. penneri, P. vulgaris, P. mirabilis, and P. myxofaciens. Results. The rpoB sequencing proved able to characterise the different species, showing six well defined rpoB sequence groups in the 1153 bp region analysed. P. myxofaciens and P. mirab…

Genome-based analyses reveal a synonymy among Halorubrum distributum Zvyagintseva and Tarasov 1989; Oren and Ventosa 1996, Halorubrum terrestre Vento…

2020

A comparative taxonomic study of Halorubrum distributum , Halorubrum terrestre , Halorubrum arcis and Halorubrum litoreum was carried out using different approaches, 16S rRNA gene sequence analysis, multilocus sequence analysis (MLSA), phylogenomic analysis based on the comparison of the core genome, orthologous average nucleotide identity (OrthoANI), Genome-to-Genome Distance Calculator (GGDC), synteny plots and polar lipid profile (PLP). The MLSA study, using the five concatenated housekeeping genes atpB, EF-2, glnA, ppsA and rpoB′, and the phylogenomic analysis based on 1347 core translated gene sequences obtained from their genomes showed that Halorubrum distributum JCM 9100T, Halorubru…

Australapatemon sp. (Trematoda) infection in Valvata macrostoma and in two leech species, Helobdella stagnalis and Erpobdella octoculata

2009

Microbial community dynamics induced by rewetting dry soil: summer precipitation matters

2015

The massive soil CO2 efflux associated with rewetting dry soils after the dry summer period significantly contributes to the annual carbon budget of Mediterranean grasslands. Rapid reactivation of soil heterotrophic activity and available carbon are both required to fuel the CO2 pulse. Better understanding of the effects of altered summer precipitation on the metabolic state of indigenous microorganisms may be important in predicting future changes in carbon cycling. We investigated the effects of a controlled rewetting event on the soil CO2 efflux pulse and on the present (DNA-based) and potentially active (rRNA-based) soil bacterial and fungal communities in intact soil cores previously s…

Wet-up response of the microbial community is shaped by soil dry-down patterns

2015

Background/Question/Methods The massive soil CO2 efflux associated with rewetting dry soils after the dry summer period significantly contributes to the annual carbon budget of Mediterranean grasslands. Rapid reactivation of soil heterotrophic activity and available carbon are both required to fuel the CO2 pulse. Better understanding of the effects of altered summer precipitation on the metabolic state of indigenous microorganisms may be important in predicting future changes in carbon cycling. We investigated the effects of a controlled rewetting event on the soil CO2efflux pulse and on the present (DNA-based) and potentially active (rRNA-based) soil bacterial and fungal communities in int…

Responses of soil bacterial and fungal communities to extreme soil drought and rewetting

2013

Question: The patterns of resource allocation and activity of the soil microbial community over the dry summer in Mediterranean grasslands are still largely unknown. The microbial response to summer desiccation reflects adaptation strategies, setting the stage for a large rainfall-induced soil CO2 pulse upon rewetting, an important component of the ecosystem carbon budget. Methods: In three California annual grasslands, the present (DNA-based) and potentially active (RNA-based) soil bacterial and fungal communities were tracked over a summer season and in response to controlled rewetting of intact soil cores. Phylogenetic marker genes for bacterial (16S) and fungal (28S) RNA and DNA were se…