Search results for "saccharomyces"

Buckwheat based substrate fermentation - possibilities for non-traditional alcoholic beverage production

2010

Characterization of hOGG1 Promoter Structure, Expression During Cell Cycle and Overexpression in Mammalian Cells

2001

Oxygen radicals are produced in all cells either by the normal cellular metabolism or by the exposure to external mutagens. The reactive oxygen species (ROS) generated can induce DNA damage. Among the principal lesions found in DNA due to ROS is an oxidized form of guanine, 8-oxo-7,8-dihydroguanine (8-oxoG). The biological relevance of this lesion has been unveiled by the study of Escherichia colt and Saccharomyces cerevisiae genes involved in the neutralization of the mutagenic effects of 8-oxoG (Cabrera et al., 1988; Nghiem et al., 1988; Radicella et al., 1988; van der Kemp et al., 1996). These genes fpg and mutY for E. colt and OGG1 for yeast, code for DNA glycosylases. Inactivation of a…

Interaction Between Osmotic and Hydrostatic Pressure in Yeast Inactivation

1999

The protective effect of an osmotic perturbation on high-pressure inactivation has been investigated using a yeast strain:Saccharomyces cerevisiae. Osmotic shocks have been carried out with binary medium (glycerol/sorbitol and water) with or without glucose. Variations of osmotic level, osmotic shift kinetics and time between osmotic and hydrostatic treatment (350 MPa/10 min) have allowed the characterization of cell baroprotection. Two different cumulative baroprotection effects have been distinguished: one conferred by the solute on biological structures and the other related to the osmotic stress, cell volume variations and stress metabolism response (HSP, trehalose). These experiments h…

The hedgehog receptor patched is involved in cholesterol transport.

2011

International audience; BACKGROUND: Sonic hedgehog (Shh) signaling plays a crucial role in growth and patterning during embryonic development, and also in stem cell maintenance and tissue regeneration in adults. Aberrant Shh pathway activation is involved in the development of many tumors, and one of the most affected Shh signaling steps found in these tumors is the regulation of the signaling receptor Smoothened by the Shh receptor Patched. In the present work, we investigated Patched activity and the mechanism by which Patched inhibits Smoothened. METHODOLOGY/PRINCIPAL FINDINGS: Using the well-known Shh-responding cell line of mouse fibroblasts NIH 3T3, we first observed that enhancement …

Influence of the storage conditions on some physicochemical and mycological parameters of honey

1994

The authors studied the changes in yeast and mould counts, pH, free and lactone acidity, colour and sugar composition undergone by honeys with water contents of 169, 188 and 220 g kg-1 on storage at refrigeration temperature (4–7°C), room temperature and 28°C for two years. Of the solid culture media assayed, honey agar and osmophilic medium fostered yeast growth while malt-extract agar and yeast-extract-malt-extract agar allowed for better development of filamentous fungi. The yeasts isolated belonged to the genera Saccharomyces, Schizosaccharomyces and Zygosaccharomyces, while filamentous moulds were of the genera Aspergillus, Penicillium, Fusarium and Alternaria. The honeys studied under…

Differential Translational Efficiency of the mRNAs Isolated from Derepressed and Glucose Repressed Saccharomyces cerevisiae

1987

Summary: Carbon catabolite derepression induced changes in the pool of yeast mRNAs translatable in a protein-synthesizing reticulocyte system. Competition experiments with globin mRNA showed that the mRNA population obtained from derepressed cells possessed a higher translational efficiency than mRNA from repressed cells. The mRNAs that could account for the high translational efficiency of the derepressed mRNA were not detected in cells growing in glucose-rich medium. Analysis of protein synthesis in the presence of 7-methylguanosine 5′-phosphate indicated that the initiation factors recognizing the 5′-terminal structure of capped messengers interacted with lower affinity with the represse…

Identification of a Functional Homolog of the Yeast Copper Homeostasis Gene ATX1 from Arabidopsis1

1998

Abstract A cDNA clone encoding a homolog of the yeast (Saccharomyces cerevisiae) gene Anti-oxidant 1(ATX1) has been identified from Arabidopsis. This gene, referred to as CopperCHaperone(CCH), encodes a protein that is 36% identical to the amino acid sequence of ATX1 and has a 48-amino acid extension at the C-terminal end, which is absent from ATX1 homologs identified in animals. ATX1-deficient yeast (atx1) displayed a loss of high-affinity iron uptake. Expression of CCH in the atx1 strain restored high-affinity iron uptake, demonstrating thatCCH is a functional homolog of ATX1. When overexpressed in yeast lacking the superoxide dismutase geneSOD1, both ATX1 and CCHprotected the cell from t…

Rauga biomasas ietekme uz aktīvo dūņu mikroorganismu fizioloģisko stāvokli benzalkonija hlorīda klātbūtnē

2019

Maģistra darba mērķis bija izvērtēt rauga Saccharomyces cerevisiae 14 ietekmi uz aktīvo dūņu baktēriju kopienas sastāvu un to aktivitāti benzalkonija hlorīda klātbūtnē. Eksperimentos ar aktīvajām dūņām un notekūdeņiem no ķīmiskās rūpnīcas, kā arī ar sintētiskajiem notekūdeņiem, tika pierādīta rauga S.cerevisiae 14 stimulējošā iedarbība uz aktīvo dūņu mikroorganismu fizioloģiskajiem procesiem, t.sk elpošanas intensitāti. Rauga ietekme uz aktīvo dūņu mikroorganismiem BAC klātbūtnē tika vērtēta pēc hidrolītisko enzīmu aktivitātes, kultivējamo mikroorganismu skaita, baktēriju kopienas sastāva u.c. Darba izstrādē tika pielietotas klasiskās mikrobioloģijas un bioķīmiskās metodes, Ion-Torrent PGM …

Análisis de los mecanismos de respuesta a bajas temperaturas y a estrés del retículo endoplásmico

2013

A lo largo de los años, la levadura S. cerevisiae se ha convertido en organismo modelo tanto en la industria, como en el campo de la investigación básica, debido a sus características metabólicas y su fácil manipulación. Es conocido que, cuando la célula se enfrenta a una situación de estrés, debe modificar su fisiología para mantener la homeostasis celular. Esta adaptación incluye la regulación del repertorio de proteínas, eliminando aquellas que ya no son necesarias o sintetizando otras nuevas. En este proceso, un orgánulo crucial es el retículo endoplásmico, que establece un punto de control en la calidad de las proteínas, ya que es el responsable de su glicosilación y correcto plegamien…

Transcriptional Rewiring, Adaptation, and the Role of Gene Duplication in the Metabolism of Ethanol of Saccharomyces cerevisiae

2020

Ethanol is the main by-product of yeast sugar fermentation that affects microbial growth parameters, being considered a dual molecule, a nutrient and a stressor. Previous works demonstrated that the budding yeast arose after an ancient hybridization process resulted in a tier of duplicated genes within its genome, many of them with implications in this ethanol “produce-accumulate-consume” strategy. The evolutionary link between ethanol production, consumption, and tolerance versus ploidy and stability of the hybrids is an ongoing debatable issue. The implication of ancestral duplicates in this metabolic rewiring, and how these duplicates differ transcriptionally, remains unsolved. Here, we …