Search results for "salmon"

Mycotoxin Incidence in Some Fish Products: QuEChERS Methodology and Liquid Chromatography Linear Ion Trap Tandem Mass Spectrometry Approach

2019

The inclusion of vegetal raw materials in feed for fish farming has increased the risk of mycotoxin occurrence in feed, as well as in edible tissues from fish fed with contaminated feed, due to the carry-over to muscle portions. Therefore, the objective of this study was to evaluate the occurrence of 15 mycotoxins in processed fish products, which are commonly consumed, such as smoked salmon and trout, different types of sushi, and gula substitutes. A QuEChERS method was employed to perform the mycotoxin extraction from fish samples. For mycotoxin identification and quantitation, the selected technique was the liquid chromatography-tandem mass spectrometry linear ion trap (LC-MS/MS-LIT). Sm…

Influence of fructose 1,6-diphosphate on the lung antioxidant defenses of mice with endotoxemia.

1990

TiO2 Supported over Hβ and HZSM-5 Photocatalysts for Salmonelle and Escherichia Coli Disinfection

2008

Soil mutagens are airborne mutagens: variation of mutagenic activities induced in Salmonella typhimurium TA 98 and TA 100 by organic extracts of agri…

2000

As our hypothesis was that soil mutagens are airborne mutagens, possibly modified by soil microorganisms, we checked solvent extracts from agricultural and forest soils collected during late summer in the environment of Mainz, a region highly charged by anthropogenic air pollution, or near Bayreuth, a rural low charged region of Germany, or in a remote region of western Corsica without anthropogenic air pollution for the presence of mutagenicity in Salmonella typhimurium. Levels of mutagenic activities were quantified by calculation of revertants/g from the initial slope of dose-response curves applying tester strains S. typhimurium TA 98 and TA 100 in the absence and presence of an activat…

SIK2 orchestrates actin-dependent host response upon Salmonella infection

2021

Significance Through conducting quantitative proteomics upon Salmonella infection, we identified a SIK2 signaling network, implementing the kinase into a so far concealed biological function. Our data exposed SIK2 as a central orchestrator of an actin regulatory network, coordinating the stability of Salmonella-containing vacuole (SCV) and cellular actin assembly, in order to limit the acute phase of the infection. Most strikingly, SIK2 is not exclusively acting locally on actin assembly associated with the SCV but impacts the actin cytoskeleton architecture in its entirety upon Salmonella infection. Our work provides a mechanistic framework for how the actin cytoskeleton is regulated and h…

Ultrahigh-Throughput Proteomics Using Fast RPLC Separations with ESI-MS/MS

2005

We describe approaches for proteomics analysis using electrospray ionization-tandem mass spectrometry coupled with fast reversed-phase liquid chromatography (RPLC) separations. The RPLC separations used 50-microm-i.d. fused-silica capillaries packed with submicrometer-sized C18-bonded porous silica particles and achieved peak capacities of 130-420 for analytes from proteome tryptic digests. When these separations were combined with linear ion trap tandem mass spectrometry measurements, approximately 1000 proteins could be identified in 50 min from approximately 4000 identified tryptic peptides; approximately 550 proteins in 20 min from approximately 1800 peptides; and approximately 250 prot…

Maternal effects in vulnerability to eye-parasites and correlations between behavior and parasitism in juvenile Arctic charr

2017

Hatchery-reared fish show high mortalities after release to the wild environment. Explanations for this include potentially predetermined genetics, behavioral, and physiological acclimation to fish farm environments, and increased vulnerability to predation and parasitism in the wild. We studied vulnerability to Diplostomum spp. parasites (load of eye flukes in the lenses), immune defense (relative spleen size) and antipredator behaviors (approaches toward predator odor, freezing, and swimming activity) in hatchery-reared juvenile Arctic charr (Salvelinus alpinus) using a nested mating design. Fish were exposed to eye-fluke larvae via the incoming water at the hatchery. Fish size was positi…

Pārtikas patogēno baktēriju sastopamība dīgstos un diedzētu sēklu produktos

2019

Pieaugot patērētāju pieprasījumam pēc minimāli apstrādātu produktu lietošanas uzturā, pārtikas drošība ir aktuāla un svarīga, īpaši tādiem termiski neapstrādātiem produktiem kā dīgsti. Eiropā Salmonella spp. un Šiga jeb verotoksīnus producējošā Escherichia coli (STEC) ir visbiežāk minētās patogēnās baktērijas, kas izraisījušas uzliesmojumus, kas asociēti ar kontaminētiem dīgstiem. STEC patogenitāti nodrošina patogenitātes gēni stx1, stx2, bet viens no virulences faktoriem kodēts gēnā eaeA. Listeria monocytogenes ir ievērojams piesārņotājs pārtikā, arīdzan tā ir tipiska augsnes baktērija. Jaunākie pētījumi apraksta ne tikai L. monocytogenes, bet arī Listeria innocua patogenitāti. Pētījumā an…

A food borne outbreak of Salmonella enterica serotype Brandenburg as a hint to compare human, animal and food isolates identified in the years 2005-2…

2011

Introduction. There are only a few reported cases of Salmonella enterica serotype Brandenburg foodborne outbreaks in the literature. In Italy Brandenburg is consistently present among the top-ten serotypes from human source, but at low prevalences. Methods. Fifty-five S. Brandenburg isolates from human, animal, environmental and food sources, including twelve isolates from a foodborne outbreak, were genotyped by PFGE. Results and Discussion. Eight pulsogroups and 19 pulsotypes were detected, with a unique pulsotype being attributed to the outbreak strains. Molecular subtyping can reliably complement the epidemiological investigations. Moreover, mapping molecular types of Salmonella isolates…

Outbreak of Salmonella enteritis bongori 48:z35:- in Sicily.

2003

Salmonella bongori 48:z 35 :- was first isolated from a lizard in Chad in 1966 and was classified as a biochemically atypical strain of the subgenus I of Kauffmann. Successively, some additional strains with different antigenic formulas but similar bioche