Search results for "script"
showing 10 items of 5143 documents
IL-28A Is a Key Regulator of T-Cell–Mediated Liver Injury via the T-Box Transcription Factor T-Bet
2006
Background & Aims: T-cell–mediated fulminant hepatitis is a potentially life-threatening event for which the underlying pathogenic mechanisms are not fully understood. Here, we demonstrate a key regulatory role of IL-28A in T-cell–mediated hepatitis. Methods: We cloned the murine IL-28A gene by reverse-transcription polymerase chain reaction, assessed the effects of recombinant IL-28A, and generated IL-28A–transgenic mice. Results: IL-28A induced TH1 cytokine production by CD4+ T lymphocytes in a T-bet–dependent manner and was up-regulated in a murine model of T-cell–mediated hepatitis upon Con A administration. In vivo, CD4+ T cells from newly created IL-28A–transgenic animals revealed an …
T cell directives for transcriptional regulation in asthma.
2003
Allergic asthma frequently starts in childhood, and environmental factors such as viruses, allergens and occupational exposure can regulate the evolution of the disease. The development of allergen-specific Th2 lymphocytes represents the triggering event for the recruitment and activation of IgE-producing B cells and fibroblasts, followed by the release of soluble factors, thus giving rise to the inflammatory reaction observed in this disease. GATA-3 was identified as a cell lineage-specific factor selectively expressed and activated in the Th2 lineage as a consequence of STAT-6 activation. However, recent literature indicates that blockade of CTLA-4-directed inhibitory signals is sufficien…
Multiple levels of MHC class I down-regulation by ras oncogenes.
1996
A number of tumours and oncogene transformed cells displayed reduced MHC class I surface expression which seemed to enable their escape from immune surveillance. To test whether oncogenic activation is directly involved in suppressing MHC class I expression, a model of inducible oncogene expression was chosen. Mouse fibroblasts transfected with different oncogenes expressed under the control of the dexamethasone-inducible MMTV promoter were analysed in the presence and absence of hormone for the mRNA and protein expression of MHC class I molecules as well as the respective oncogenes. Immunofluorescence analyses demonstrated an inverse association of MHC class I and oncogene expression after…
Transcriptomic Changes Following Partial Depletion of CENP-E in Normal Human Fibroblasts
2021
The centromere is a fundamental chromosome structure in which the macro-molecular kinetochore assembles and is bound by spindle microtubules, allowing the segregation of sister chromatids during mitosis. Any alterations in kinetochore assembly or functioning or kinetochore–microtubule attachments jeopardize chromosome stability, leading to aneuploidy, a common feature of cancer cells. The spindle assembly checkpoint (SAC) supervises this process, ensuring a faithful segregation of chromosomes. CENP-E is both a protein of the kinetochore and a crucial component of the SAC required for kinetochore–microtubule capture and stable attachment, as well as congression of chromosomes to the metaphas…
3D digital tools for the archaeological massive artifacts documentation
2022
This study focuses on 3D acquisition and documentation procedures, innovating and implementing traditional survey methods to promote and increase the efficacy of the historical and archaeological heritage fruition. Especially within museum itineraries, modern digital technologies, if consistently applied, can promote a better interpretative reading, implementing the knowledge of scholars. Moreover, in coherence with the Horizon Europe “Cultural Heritage” area of intervention, for a new future inextricably linked to the history of Cultural Heritage, it is essential to experiment approaches that favor innovative mechanisms of fruition and promotion of works of art, based on the idea that the …
Defense Responses in Two Ecotypes of Lotus japonicus against Non-Pathogenic Pseudomonas syringae
2013
Lotus japonicus is a model legume broadly used to study many important processes as nitrogen fixing nodule formation and adaptation to salt stress. However, no studies on the defense responses occurring in this species against invading microorganisms have been carried out at the present. Understanding how this model plant protects itself against pathogens will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important defense mechanisms activated upon bacterial attack, we explored in this work the main responses occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. ja…
Explaining Individual and Collective Programming Students’ Behavior by Interpreting a Black-Box Predictive Model
2021
Predicting student performance as early as possible and analysing to which extent initial student behaviour could lead to failure or success is critical in introductory programming (CS1) courses, for allowing prompt intervention in a move towards alleviating their high failure rate. However, in CS1 performance prediction, there is a serious lack of studies that interpret the predictive model’s decisions. In this sense, we designed a long-term study using very fine-grained log-data of 2056 students, collected from the first two weeks of CS1 courses. We extract features that measure how students deal with deadlines, how they fix errors, how much time they spend programming, and so forth. Subs…
EXPRESSION OF T-CELL TRANSCRIPTION FACTOR T-BET IS INCREASED IN COPD
2010
Cost-description and multiple imputation of missing values: theSATisfaction and adherence to COPD treatment(SAT) study
2018
Aim:This article reports on a retrospective quarterly cost description (CD) performed on 401 patients with stable chronic obstructive pulmonary disease (COPD) at enrolment in the national, multicen...
An Information Systems Design Product Theory for Software Project Estimation and Measurement Systems
2009
There is relatively little research on software Project Estimation and Measurement Systems (PEMS). Commercial PEMS vary in functionality and effective- ness. Their intended users thus do not know what to ex- pect from PEMS and how to evaluate them. This paper creates an information system design product theory for the class of PEMS that prescribes the meta-requirements, the meta-design, and applicable theories for all products within the class. Meta-requirements and the meta-design are derived from the project estimation and measurement literature, experiences obtained during more than ten years of empirical work in Finnish Software Measurement Association, and a commercially available PEMS.