Search results for "script"
showing 10 items of 5143 documents
Induction of interferon regulatory factors, 2′‐5′ oligoadenylate synthetase, P68 kinase and RNase L in chronic myelogenous leukaemia cells and its re…
1996
The genes crucially determining the therapeutic response of chronic myelogenous leukaemia (CML) to interferon-alpha (IFN-alpha) are unknown. Recently, two independent IFN-alpha signalling pathways were identified: the classic pathway mediates induction of 2'-5' oligoadenylate synthetase (2-5 OAS), p68 kinase and IFN regulatory factor-2 (IRF-2), whereas the alternate pathway leads to activation of IFN regulatory factor-1 (IRF-1). We investigated whether deficient or imbalanced expression of components of these two pathways is associated with resistance of CML cells to antiproliferative action of IFN alpha/beta. Constitutive and IFN-induced transcript levels of IFN-dependent genes in mononucl…
Cyclic adenosine monophosphate is a key component of regulatory T cell–mediated suppression
2007
Naturally occurring regulatory T cells (T reg cells) are a thymus-derived subset of T cells, which are crucial for the maintenance of peripheral tolerance by controlling potentially autoreactive T cells. However, the underlying molecular mechanisms of this strictly cell contact–dependent process are still elusive. Here we show that naturally occurring T reg cells harbor high levels of cyclic adenosine monophosphate (cAMP). This second messenger is known to be a potent inhibitor of proliferation and interleukin 2 synthesis in T cells. Upon coactivation with naturally occurring T reg cells the cAMP content of responder T cells is also strongly increased. Furthermore, we demonstrate that natur…
Distribution and kinetics of superantigen-induced cytokine gene expression in mouse spleen.
1993
The polyclonal stimulation of T cells by bacterial superantigens is involved in the pathogenesis of the toxic shock syndrome in certain staphylococcal and streptococcal infections. Here we describe the onset and kinetics of superantigen-induced cytokine production in situ in spleens of normal BALB/c mice monitored at the level of cytokine mRNA expression by in situ hybridization. Messenger RNAs for interleukin 2 (IL-2), interferon gamma, and tumor necrosis factors (TNF) alpha and beta were not expressed at detectable levels in spleens of unstimulated animals but became visible already 30 min after intraperitoneal application of 50 micrograms staphylococcal enterotoxin B. All mRNA levels sho…
IL-13 receptor α 2: A regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts
2005
Background IL-13 and IL-4 share many functional properties as a result of their use of a common receptor complex comprising IL-13 receptor α 1 (IL-13Rα1) and IL-4 receptor α (IL-4Rα). The nonsignaling receptor IL-13 receptor α 2 (IL-13Rα2) binds IL-13 with high affinity and specificity and is believed to be a decoy receptor for IL-13. Objective We sought to examine the inhibitory effects of soluble and membrane-bound IL-13Rα2 on IL-13– and IL-4–mediated effects. Methods Primary human fibroblasts were grown from endobronchial biopsy specimens obtained from volunteers. Upregulation of IL-13Rα2 mRNA was measured by means of RT-PCR, and the level of surface expression was measured by means of F…
Inhibition of Delayed-Type Hypersensitivity by Cucurbitacin R through the Curbing of Lymphocyte Proliferation and Cytokine Expression by Means of Nuc…
2009
Cucurbitacin R is known to exhibit an anti-inflammatory effect in different experimental models of inflammation. In this article, we outline the effect of cucurbitacin R on T lymphocyte proliferation, cytokine production, and nuclear factor activation, as well as its influence on various experimental models of delayed-type hypersensitivity (DTH) in mice. Cucurbitacin R reduced the proliferation of phytohemagglutinin A-stimulated human T lymphocytes (IC(50), 18 microM), modifying the cell cycle, as well as the production of cytokines [interleukin (IL)-2, IL-4, IL-10, and especially interferon-gamma] and the induction of the principal cyclins implicated in the cell cycle (A(1), B(1), D(2), an…
Adaptive semantic web based environment for web resources
2008
Tulevaisuuden kaikkialle ulottuvassa internetissä tietojärjestelmät kommunikoivat paitsi käyttäjien kanssa, myös toisten sovellusten ja instrumentoitujen laitteiden kanssa. Tämän dynaamisen ja heterogeenisen digitaalisen ympäristön hallinnoimiseksi ja hyödyntämiseksi käyttämiemme laitteiden tulisi olla nykyistä proaktiivisempia ja tiedon tulisi olla kuvattu nykyistä kontekstitietoisemmalla tavalla.Lisäksi tulevaisuuden verkon resurssit tulisi kyetä kuvaamaan semanttisesti, jotta eri resurssit voidaan löytää ja sovittaa yhteen automaattisesti. Tämä mahdollistaa myös päätelmien tekemisen järjestelmien tiedoista sekä monimutkaisen kokonaisuuden komponenttien käyttäytymisen ohjaamisen helpommin…
Enabling Primary and Specialist Care Interoperability Through HL7 CDA Release 2 and the Chronic Care Model: An Italian Case Study
2012
Interoperability is the key to enable clinical information systems for General Practitioners (GP) and Hospital Specialists (HS) in order to exchange and manage the Chronic Care Models (CCM) medical records, Patient Summary (PS), and Electronic Prescription (e-Prescription) documents while accessing the electronic health record. We present a localization experience for PS and e-prescription, based on the Health Level Seven Version 3 Clinical Document Architecture Release 2, developed for Italian healthcare. We describe also an experience on the implementation of CCM for sharing patient clinical data among healthcare providers in the management of diagnostic and therapeutic pathways for chron…
Rough Pragmatic Description Logic
2013
In this chapter, a rough description logic is built on the basis of a pragmatic standpoint of representation of knowledge. The pragmatic standpoint has influenced the acceptance of a broader definition of the semantic network than that appearing in the literature. The definition of the semantic network is a motivation of the introduced semantics of the language of the descriptive logic. First, the theoretical framework of representation of knowledge that was proposed in the papers [24,25] is adjusted to the description of data processing. The pragmatic system of knowledge representation is determined, as well as situations of semantic adequacy and semantic inadequacy for represented knowled…
Eukaryotic snoRNAs: a paradigm for gene expression flexibility.
2009
AbstractSmall nucleolar RNAs (snoRNAs) are one of the most ancient and numerous families of non-protein-coding RNAs (ncRNAs). The main function of snoRNAs – to guide site-specific rRNA modification – is the same in Archaea and all eukaryotic lineages. In contrast, as revealed by recent genomic and RNomic studies, their genomic organization and expression strategies are the most varied. Seemingly snoRNA coding units have adopted, in the course of evolution, all the possible ways of being transcribed, thus providing a unique paradigm of gene expression flexibility. By focusing on representative fungal, plant and animal genomes, we review here all the documented types of snoRNA gene organizati…
A comparison of the transcriptome of Drosophila melanogaster in response to entomopathogenic fungus, ionizing radiation, starvation and cold shock
2015
Background The molecular mechanisms that determine the organism's response to a variety of doses and modalities of stress factors are not well understood. Results We studied effects of ionizing radiation (144, 360 and 864 Gy), entomopathogenic fungus (10 and 100 CFU), starvation (16 h), and cold shock (+4, 0 and -4°C) on an organism's viability indicators (survival and locomotor activity) and transcriptome changes in the Drosophila melanogaster model. All stress factors but cold shock resulted in a decrease of lifespan proportional to the dose of treatment. However, stress-factors affected locomotor activity without correlation with lifespan. Our data revealed both significant similarities …