Search results for "script"
showing 10 items of 5143 documents
Regulatory T Cells and IL-10 Independently Counterregulate Cytotoxic T Lymphocyte Responses Induced by Transcutaneous Immunization
2011
Background: The imidazoquinoline derivate imiquimod induces inflammatory responses and protection against transplanted tumors when applied to the skin in combination with a cognate peptide epitope (transcutaneous immunization, TCI). Here we investigated the role of regulatory T cells (Treg) and the suppressive cytokine IL-10 in restricting TCI-induced cytotoxic T lymphocyte (CTL) responses. Methodology/Principal Findings: TCI was performed with an ointment containing the TLR7 agonist imiquimod and a CTL epitope was applied to the depilated back skin of C57BL/6 mice. Using specific antibodies and FoxP3-diphteria toxin receptor transgenic (DEREG) mice, we interrogated inhibiting factors after…
Spatial coherence and potential predictability of intraseasonal descriptors of the rainy season in Soudano-Sahelian Africa : application to the pearl…
2010
The aim of this thesis is twofold : (i) fill a lack of knowledge about the spatial coherence and seasonal predictability of the intraseasonal characteristics (ISC) of the soudano-sahelian rainy season. These ISC are the seasonal rainfall amount (S), the daily rainfall frequency > 1 mm (O), the daily rainfall mean intensity (I), the dates of the onset and withdrawal of the rainy season, the mean length of the dry (LDS) and/or wet spells (LWS) ; (ii) document the climate-agriculture relationship over the Niamey area based on the millet crop example.From FRIEND-AOC daily rainfall records (1950-2000) for a 136 stations-network located in Senegal, Mali, Burkina-Faso and Niger, the spatial cohere…
Du XIIe au XXe siècle, une fresque historique à reconstituer à l’abbaye de Noirlac (Cher)
2022
La politique de prescription menée depuis la fin des années 2000 vise à mettre en cohérence l’apport de nombreuses décennies de recherche archéologique (fouilles anciennes puis préventives) sur un site emblématique. Les perspectives étaient triples : disposer de l’ensemble des éléments de décision pour accompagner le projet d’aménagement paysager ; valoriser l’apport de l’archéologie à la connaissance de Noirlac ; penser un outil pouvant orienter la réflexion sur les aménagements futurs.
Recherches sur l’histoire de Troyes, des origines à la fin du Moyen Âge
2022
Augustobona chef-lieu de la cité des Tricasses, puis la ville de Troyes se sont développées en aval de la confluence de la Seine et de la Vienne, un de ses modestes affluents en rive gauche, à l’endroit où le fleuve quitte les terrains argileux de la Champagne humide pour les terrains crayeux de la Champagne sèche. De nombreuses sources historiques et archéologiques montrent que les cours actuels des bras de la Seine et de la Vienne ont considérablement fluctué depuis la fin de la Protohistoire, tant par migration naturelle que sous l’effet de travaux de canalisation ou de dérivation. Dans un premier temps (1981-2020), l’activité archéologique préventive paraît conditionnée par l’historiogr…
Du vicus de la Canche au castrum de Montreuil, un chaînon manquant: le fiscus d'Attin?
2010
Stéphane Lebecq, Bruno Béthouart et Laurent Verslype, avec la collaboration de Laurence Dieryck et Inès Leroy; International audience
Principle considerations for the use of transcriptomics in doping research
2011
Over the course of the past decade, technical progress has enabled scientists to investigate genome-wide RNA ex- pression using microarray platforms. This transcriptomic approach represents a promising tool for the discovery of basic gene expression patterns and for identification of cellular signalling pathways under various conditions. Since doping substances have been shown to influence mRNA expression, it has been suggested that these changes can be detected by screening the blood transcriptome. In this review, we critically discuss the potential but also the pitfalls of this application as a tool in doping research. Transcriptomic approaches were considered to potentially provide resea…
Small genomes and the difficulty to define minimal translation and metabolic machineries
2015
The notion of minimal life has sparked the interest of scientists in different fields, ranging from the origin-of-life research to biotechnology-oriented synthetic biology. Whether the interest is focused on the emergence of protocells out of prebiotic systems or the design of a cell chassis ready to incorporate new devices and functions, proposing minimal combinations of genes for life is not a trivial task. Using comparative genomics and biochemistry of endosymbionts (i.e., intracellular mutualistic symbionts) and intracellular parasites, a decade ago we proposed the core of a minimal gene set for a simple heterotrophic cell adapted to a chemically complex environment. In this work, we di…
Concept and Development of Modular VLIW Processor Based on FPGA
2010
Modern FPGA chips, with their larger memory capacity and reconfigurability potential, are opening new frontiers in rapid prototyping of embedded systems. With the advent of high density FPGAs it is now possible to implement a high performance VLIW processor core in an FPGA. Architecture based on Very Long Instruction Word (VLIW) processors are an optimal choice in the attempt to obtain high performance level in embedded system. In VLIW architecture, the effectiveness of these processors depends on the ability of compilers to provide sufficient instruction level parallelism(ILP) in program code. Using advanced compiler technology could take these functions, This paper describes research resu…
Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) as a user-friendly system to detect SARS-CoV-2 infection: a multicentric study
2021
Although reverse transcriptase quantitative PCR remains the gold standard to perform viral detection, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is already used to perform diagnosis of various infections. This work reports the results of a multicentric study performed in Sicily to evaluate the diagnostic power of an RT-LAMP kit for the diagnosis of SARS-CoV-2 infection on a total of 551 samples collected in January and February 2021, revealing sensitivity, specificity, accuracy, positive and negative predictive values ≥95%. Our results suggest the potential employment of this kit as a screening test to be used where fast and reliable results are demanded without …
The gut microbiota instructs the hepatic endothelial cell transcriptome
2021
Summary The gut microbiota affects remote organ functions but its impact on organotypic endothelial cell (EC) transcriptomes remains unexplored. The liver endothelium encounters microbiota-derived signals and metabolites via the portal circulation. To pinpoint how gut commensals affect the hepatic sinusoidal endothelium, a magnetic cell sorting protocol, combined with fluorescence-activated cell sorting, was used to isolate hepatic sinusoidal ECs from germ-free (GF) and conventionally raised (CONV-R) mice for transcriptome analysis by RNA sequencing. This resulted in a comprehensive map of microbiota-regulated hepatic EC-specific transcriptome profiles. Gene Ontology analysis revealed that …