Search results for "script"
showing 10 items of 5143 documents
A feedback mechanism controls rDNA copy number evolution in yeast independently of natural selection.
2022
Ribosomal DNA (rDNA) is the genetic loci that encodes rRNA in eukaryotes. It is typically arranged as tandem repeats that vary in copy number within the same species. We have recently shown that rDNA repeats copy number in the yeast Saccharomyces cerevisiae is controlled by cell volume via a feedback circuit that senses cell volume by means of the concentration of the free upstream activator factor (UAF). The UAF strongly binds the rDNA gene promoter, but is also able to repress SIR2 deacetylase gene transcription that, in turn, represses rDNA amplification. In this way, the cells with a smaller DNA copy number than what is optimal evolve to increase that copy number until they reach a numb…
RNA-controlled nucleocytoplasmic shuttling of mRNA decay factors regulates mRNA synthesis and a novel mRNA decay pathway.
2021
AbstractmRNA level is controlled by factors that mediate both mRNA synthesis and decay, including the 5’ to 3’ exonuclease Xrn1. Here we show that nucleocytoplasmic shuttling of several yeast mRNA decay factors plays a key role in determining both mRNA synthesis and decay. Shuttling is regulated by RNA-controlled binding of the karyopherin Kap120 to two nuclear localization sequences (NLSs) in Xrn1, location of one of which is conserved from yeast to human. The decaying RNA binds and masks NLS1, establishing a link between mRNA decay and Xrn1 shuttling. Preventing Xrn1 import, either by deleting KAP120 or mutating the two Xrn1 NLSs, compromises transcription and, unexpectedly, also cytoplas…
PROX1 transcription factor controls rhabdomyosarcoma growth, stemness, myogenic properties and therapeutic targets
2022
Funding Information: ACKNOWLEDGMENTS. We would like to thank Dr. Tuomas Tammela and Dr. Monika Ehnmann for providing RMS cell lines and Dr. Jenny Högström for discussions and comments during the project. Kirsi Mattinen, Jefim Brodkin, Maxime Laird, Manon Gruchet, Ilse Paetau, Tanja Laakkonen, and Tapio Tainola are acknowledged for their excellent technical help. We also thank the Laboratory Animal Center at the University of Helsinki for expert animal care, the Biomedicum Imaging Unit for microscope support, the Biomedicum Functional Genomics Unit for the RNAseq experiments and the FIMM Technology Centre High Throughput Biomedicine for the drug sensitivity and resistance testing. Our first …
Accessible On-Line Floor Plans
2015
Better access to on-line information graphics is a pressing need for people who are blind or have severe vision impairment. We present a new model for accessible presentation of on-line information graphics and demonstrate its use for presenting floor plans. While floor plans are increasingly provided on-line, people who are blind are at best provided with only a high-level textual description. This makes it difficult for them to understand the spatial arrangement of the objects on the floor plan. Our new approach provides users with significantly better access to such plans. The users can automatically generate an accessible version of a floor plan from an on-line floor plan image quickly …
Designing and analyzing collaboration in a scripted game for vocational education
2008
This study attempts to combine the technological possibilities of 3D-game environments and collaborative learning scripts. The study is a design experiment (N=64) with multiple data collection and analysis (quantitative and qualitative) methods. The aims were twofold: The aim was to develop a game environment to simulate issues of work safety in a vocational context and to answer the following questions on the basis of an empirical study: (1) What kind of activities did the scripted game environment generate among the players? (2) How did the least and the most successful groups differ in this respect despite the same scripted game environment? Findings indicated that scripted game environm…
The SUMMA Platform: A Scalable Infrastructure for Multi-lingual Multi-media Monitoring
2018
The open-source SUMMA Platform is a highly scalable distributed architecture for monitoring a large number of media broadcasts in parallel, with a lag behind actual broadcast time of at most a few minutes. The Platform offers a fully automated media ingestion pipeline capable of recording live broadcasts, detection and transcription of spoken content, translation of all text (original or transcribed) into English, recognition and linking of Named Entities, topic detection, clustering and crosslingual multi-document summarization of related media items, and last but not least, extraction and storage of factual claims in these news items. Browser-based graphical user interfaces provide humans…
ID: 186
2015
In the past years, and clear pathogenic role was shown for Th17 cells in the development of autoimmune diseases. In particular, these cells were shown to play a critical roIn the past years, and clear pathogenic role was shown for Th17 cells in the development of autoimmune diseases. In particular, these cells were shown to play a critical role in the development of experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. One of the major cytokines Th17 cells produce is IL-17A, a cytokine of the IL-17 family. IL-17A, as well as it homologue IL-17F bind and trigger cells via the IL-17 receptor A/C complex. We have used a series of mice with deficiencies in the…
NFAT1 deficit and NFAT2 deficit attenuate EAE via different mechanisms
2015
EAE serves as an animal model for multiple sclerosis and is initiated by autoreactive T cells that infiltrate the CNS. Recognition of myelin-associated Ags within the CNS leads to activation of the transcription factor family NFAT. Here, we demonstrate an essential role for NFAT in disease induction, as the combined lack of NFAT1 (NFATc2) and NFAT2 (NFATc1) completely protected mice. Single deficiency of either NFAT1 or NFAT2 ameliorated the course of EAE, and NFAT2 ablation resulted in an obstructed proinflammatory reaction. However, NFAT1 deficit led to an anti-inflammatory response with nonpathogenic Th17 and Th2 cells concurrently secreting IL-17, IL-4, and IL-10. Both IL-4 and IL-10 co…
Scripting the Rules for Mars and Venus: Advice Literature and Online Dating
2007
An Amazon.com search for ‘guide to online dating’ produces tens of hits ranging from Online Dating for Dummies and Complete Idiot’s Guide to Online Dating and Relating to titles targeted at Christians, teens, seniors, lesbian, gay, bisexual, and straight users, and people in recovery. This plethora of advice literature – in addition to novels, films, TV programmes, and articles on the topic – corresponds to the multitude of online dating services developed during the past decade. The books outline basic principles of online dating, available solutions, and netiquette. In addition, they provide guides in self-improvement in the tradition of self-help literature, which according to Lichterman…
A Multivariate Analysis on Non-nucleoside HIV-1 Reverse Transcriptase Inhibitors and Resistance Induced by Mutation
2003
This paper describes the use of multivariate statistical procedure PCA as a tool to explore the inhibitory activity of classes of NNRTIs against HIV-1 viruses (wild type and more frequent mutants, Y181C, V106A, K103N, L100I) and against RT enzyme. The analysis of correlations between biological activity and molecular descriptors or similarity indexes allowed a reliable classification of the fifty five derivatives considered in this study. The best results were obtained in the case of L100I and K103N mutants for which the higher number of assignments was found when the principal components derived from the descriptors were used. On this basis this statistical approach is proposed as a reliab…