Search results for "sensitive"
showing 10 items of 210 documents
In situ formation of pyronin dyes for fluorescence protease sensing
2017
International audience; We report a reaction-based strategy for the fluorogenic detection of protease activity. Based on the "covalent-assembly" probe design principle recently put forward by the Yang group for detection of Sarin related threats (J. Am. Chem. Soc., 2014, 136, 6594-6597), we have designed two unusual nonfluorescent caged precursors (mixed bis-aryl ethers) which are readily converted into a fluorescent unsymmetrical pyronin dye through a domino cyclisation-aromatisation reaction triggered by penicillin G acylase (PGA) or leucine aminopeptidase (LAP). Fluorescence-based in vitro assays and HPLCfluorescence/- MS analyses support the claimed activation mechanism whose the furthe…
Precision luminosity measurements at LHCb
2014
Measuring cross-sections at the LHC requires the luminosity to be determined accurately at each centre-of-mass energy $\sqrt{s}$. In this paper results are reported from the luminosity calibrations carried out at the LHC interaction point 8 with the LHCb detector for $\sqrt{s}$ = 2.76, 7 and 8 TeV (proton-proton collisions) and for $\sqrt{s_{NN}}$ = 5 TeV (proton-lead collisions). Both the "van der Meer scan" and "beam-gas imaging" luminosity calibration methods were employed. It is observed that the beam density profile cannot always be described by a function that is factorizable in the two transverse coordinates. The introduction of a two-dimensional description of the beams improves sig…
Variability and polarization in the inner jet of 3C 395
2001
We present new results on the parsec-scale jet of the quasar 3C395, derived from VLBI polarization sensitive observations made in 1995.91 and 1998.50 at 8.4, 15.4 and 22.2 GHz. The observations show a complex one-sided jet extending up to 20 mas, with a projected magnetic field essentially aligned with the radio jet. The emission is strongly dominated, in total intensity and polarization, by the core and the inner jet region (of ~3 mas length). We have studied the details of this dominant region finding clear structural variations during this ~2.5 years period, in contrast with the apparent quietness of the jet structure inferred from lower resolution VLBI observations. We observe the eject…
Adaptive semantic web based environment for web resources
2008
Tulevaisuuden kaikkialle ulottuvassa internetissä tietojärjestelmät kommunikoivat paitsi käyttäjien kanssa, myös toisten sovellusten ja instrumentoitujen laitteiden kanssa. Tämän dynaamisen ja heterogeenisen digitaalisen ympäristön hallinnoimiseksi ja hyödyntämiseksi käyttämiemme laitteiden tulisi olla nykyistä proaktiivisempia ja tiedon tulisi olla kuvattu nykyistä kontekstitietoisemmalla tavalla.Lisäksi tulevaisuuden verkon resurssit tulisi kyetä kuvaamaan semanttisesti, jotta eri resurssit voidaan löytää ja sovittaa yhteen automaattisesti. Tämä mahdollistaa myös päätelmien tekemisen järjestelmien tiedoista sekä monimutkaisen kokonaisuuden komponenttien käyttäytymisen ohjaamisen helpommin…
Large Scale Knowledge Matching with Balanced Efficiency-Effectiveness Using LSH Forest
2017
Evolving Knowledge Ecosystems were proposed to approach the Big Data challenge, following the hypothesis that knowledge evolves in a way similar to biological systems. Therefore, the inner working of the knowledge ecosystem can be spotted from natural evolution. An evolving knowledge ecosystem consists of Knowledge Organisms, which form a representation of the knowledge, and the environment in which they reside. The environment consists of contexts, which are composed of so-called knowledge tokens. These tokens are ontological fragments extracted from information tokens, in turn, which originate from the streams of information flowing into the ecosystem. In this article we investigate the u…
Amor y erotismo en Vargas Llosa y su traducción al inglés
2017
En este artículo analizaremos uno de los ejes de la creación novelística de Mario Vargas Llosa (la exploración del amor y el sexo) a través de las traducciones inglesas de las novelas Pantaleón y las visitadoras (1973), La tía Julia y el escribidor (1977), Elogio de la madrastra (1988) y Los cuadernos de don Rigoberto (1997). Si bien en casi todas las obras de Vargas Llosa el amor juega un papel relevante, en estas cuatro obras hay una fabulación explícita sobre la sexualidad humana y un notable trabajo sobre la elaboración lingüística y retórica del lenguaje del amor y el sexo. Traducir el lenguaje relativo al sexo es, sin duda, un área muy sensible, el lugar de confluencia entre las inter…
Radial and peripheral clines in northern polymorphic populations of Philaenus spumarius
2009
Geographic variability in the colour polymorphism of Philaenus spumarius (Homoptera) was investigated in the northern parts of Finland, Norway and Sweden. The investigation was directed mainly at peripheral populations south of the Baltic—Arctic divide. The colour phenotypes were found to differ in their “north tolerance”. The phenotypes typ (pt/pt) and lat (pt/pt) were the most, and the phenotype tri (pt/pT) the least tolerant to marginal conditions. Differences in “north tolerance” between the phenotypes cause radial clines to be present in allele frequencies. The radial clines may be caused by the combined effect of several different factors. These include shortness of the thermal growth…
An in vitro and in vivo study of peptide-functionalized nanoparticles for brain targeting: The importance of selective blood-brain barrier uptake
2017
Targeted delivery of drugs across endothelial barriers remains a formidable challenge, especially in the case of the brain, where the blood-brain barrier severely limits entry of drugs into the central nervous system. Nanoparticle-mediated transport of peptide/protein-based drugs across endothelial barriers shows great potential as a therapeutic strategy in a wide variety of diseases. Functionalizing nanoparticles with peptides allows for more efficient targeting to specific organs. We have evaluated the hemocompatibilty, cytotoxicity, endothelial uptake, efficacy of delivery and safety of liposome, hyperbranched polyester, poly(glycidol) and acrylamide-based nanoparticles functionalized wi…
Single mechano-gated channels activated by mechanical deformation of acutely isolated cardiac fibroblasts from rats
2010
Aim Mechanosensitive conductances were reported in cardiac fibroblasts, but the properties of single channels mediating their mechanosensitivity remain uncharacterized. The aim of this work was to investigate single mechano-gated channels (MGCs) activated by mechanical deformations of cardiac fibroblasts. Methods Currents through single MGCs and mechanosensitive whole-cell currents were recorded from isolated rat atrial fibroblasts using the cell-attached and whole-cell patch-clamp configurations respectively. Defined mechanical stress was applied via the patch pipette used for the whole-cell recordings. Results Under resting conditions occasional short openings of two types of single MGCs …
Synthesis and antiproliferative activity of [1,2,3,5]tetrazino[5,4-a]indoles, a new class of azolo-tetrazinones.
2004
Eight derivatives of the new ring system [1,2,3,5]tetrazino[5,4-a]indole-4-one 7, were synthesised in good yields by reaction of 2-diazoindoles with alkyl or aryl isocyanates. Compounds 7 were screened at National Cancer Institute (NCI) for their activity against a panel of approximately 60 human tumour cell lines. Some of them showed antiproliferative activity having generally GI50 in the micromolar range. The most sensitive cell lines were SF-295, SNB-75 and SF-539 of the CNS cancer sub-panel, SR of the leukaemia sub-panel, UACC-62 of the melanoma sub-panel and OVCAR-4 of the ovarian cancer sub-panel. 2004 Elsevier Ltd. All rights reserved.