Search results for "sensitive"
showing 10 items of 210 documents
Determination of 14-3-3 protein levels in cerebrospinal fluid from Creutzfeldt-Jakob patients by a highly sensitive capture assay.
2001
The level of 14-3-3gamma protein was determined in the cerebrospinal fluid (CSF) from patients with Creutzfeldt-Jakob disease (CJD) and non-CJD patients applying a new and fast microplate assay (14-3-3 protein capture assay), based on the binding to a peptide comprising a phosphorylated recognition motif of 14-3-3 protein. The levels of the gamma-isoform of 14-3-3 protein in CSF samples from CJD patients (n=41) were significantly higher than those observed in patients with non-CJD dementias (n=36) suggesting that this capture assay is a reliable method in the diagnosis of CJD. Since this assay allows the direct measurement of 14-3-3 protein in the CSF without prior concentration it is an ea…
Agent-based modelling of values: the case of value sensitive design for refugee logistics
2020
We have used value sensitive design as a method to develop an agent-based model of values in humanitarian logistics for refugees. Schwartz's theory of universal values is implemented in the model in such a way that agents can make value trade-offs, which are operationalized into a measure of refugee wellbeing and a measure of public opinion about how the refugee logistics is being handled. By trying out different ‘value-scenarios’, stakeholders who are responsible for, or involved in refugee logistics can have insights into the effects of various value choices. The model is visualized and made usable as a platform (interactive website) for decision-makers to understand the trade-offs in pol…
An original approach for gas chromatography-olfactometry detection frequency analysis: Application to gin
2012
Abstract Gas Chromatography-Olfactometry (GC-O) is a technique that lies halfway between physicochemical and sensory analysis and involves the perception of volatile flavour compounds, separated by gas chromatography, by the human nose. Of the different GC-O procedures available, detection frequency has been proved to be more rapid and more repeatable, even with an untrained panel. This characteristic regarding the panel is often not considered when dealing with the sensory attributes determined by assessors. An original approach to GC-O using the detection frequency procedure has been developed and tested on two types of gin and made it possible to benefit from sensory data. The panel cons…
Temporary occlusions of coronaries arteries by thermoreversible gel : leGoo.To experimental to human case
2011
ObjectifsThe objective of this work is to assess the feasibility of using the LeGoo gel to occlude the coronary arteries temporarily, to facilitate the performance of coronary bypass (off-pump technique), initially in pigs then in human. A Secondary objective was to evaluate the impact of the injection on the endothelium and myocardium.MethodesThe first study evaluated in pigs the feasibility of using LeGoo, while performing coronary bypass on the left anterior descending or on the right coronary artery and to evaluate the endothelial function of coronary arteries occluded by the gel.The second study analyzed endothelial function of internal thoracic arteries, in the area occluded by the ge…
La toilette, une expérience du "sensible"
2011
What if the act of bathing patients was more important than a simple act of basic care? Information and communication sciences enable an analysis rich in signifi cations to be carried out, while unveiling new dimensions of understanding. Those of sensation, sensitivity and the symbolic dimension give back to this act of nursing care a real importance in the fi eld of the relationship. Giving meaning to practices means revealing new inter-relational areas to the benefi t of the patient.
Equivalence of Open-Loop and Closed-Loop Operation of SAW Resonators and Delay Lines
2019
International audience; Surface acoustic wave (SAW) sensors in the form of two-port resonators or delay lines are widely used in various fields of application. The readout of such sensors is achieved by electronic systems operating either in an open-loop or in a closed-loop configuration. The mode of operation of the sensor system is usually chosen based on requirements like, e.g., bandwidth, dynamic range, linearity, costs, and immunity against environmental influences. Because the limit of detection (LOD) at the output of a sensor system is often one of the most important figures of merit, both readout structures, i.e., open-loop and closed-loop systems, are analyzed in terms of the minim…
Relationships and linguistically sensitive teaching : Developing teacher practicum at Åbo Akademi University
2020
Project “Linguistically sensitive teaching in all classrooms” (Listiac) aims to make an impact on initial teacher education systems in Europe through action research. In line with European policies, the seven partner countries including Finland, Spain (the autonomous Basque country and Catalonia), Belgium, France, Slovenia, Lithuania and Portugal work together in providing competences and skills needed for handling linguistic diversity in our schools. Diversity education has been regarded nearly everywhere as an isolated ‘add-on’ course or study module (EC 2017). Thus, it has remained insufficiently mainstreamed as an integrated component of teaching practice. Listiac addresses this imbalan…
Study of the aminopeptidase N gene family in the lepidopterans Ostrinia nubilalis (Hübner) and Bombyx mori (L.): Sequences, mapping and expression
2010
Aminopeptidases N (APNs) are a class of ectoenzymes present in lepidopteran larvae midguts, involved in the Bacillus thuringiensis (Bt) toxins mode of action. In the present work, seven aminopeptidases have been cloned from the midgut of Ostrinia nubilalis, the major Lepidopteran corn pest in the temperate climates. Six sequences were identified as APNs because of the presence of the HEXXH(X)18E and GAMEN motifs, as well as the signal peptide and the GPI-anchor sequences. The remaining sequence did not contain the two cellular targeting signals, indicating it belonged to the puromycin-sensitive aminopeptidase (PSA) family. An in silico analysis allowed us to find orthologous sequences in Bo…
Long-Term Potentiation in the Neonatal Rat Barrel Cortex In Vivo
2012
Long-term potentiation (LTP) is important for the activity-dependent formation of early cortical circuits. In the neonatal rodent barrel cortex, LTP has been studied only in vitro . We combined voltage-sensitive dye imaging with extracellular multielectrode recordings to study whisker stimulation-induced LTP in the whisker-to-barrel cortex pathway of the neonatal rat barrel cortex in vivo . Single whisker stimulation at 2 Hz for 10 min induced an age-dependent expression of LTP in postnatal day (P) 0 to P14 rats, with the strongest expression of LTP at P3–P5. The magnitude of LTP was largest in the activated barrel-related column, smaller in the surrounding septal region, and no LTP could b…
Chromatin structure of the 5′ flanking region of the yeastLEU2 gene
1989
The chromatin structure of theLEU2 gene and its flanks has been studied by means of nuclease digestion, both with micrococcal nuclease and DNase I. The gene is organized in an array of positioned nucleosomes. Within the promoter region, the nucleosome positioning places the regulatory sequences, putative TATA box and upstream activator sequence outside the nucleosomal cores. The tRNA3 Leu gene possesses a characteristic structure and is protected against nucleases. Most of the 5′ flank is sensitive to DNase I digestion, although no clear hypersensitive sites were found. The chromatin structure is independent of either the transcriptional state of the gene or the chromosomal or episomal loca…