Search results for "sequence"
showing 10 items of 4987 documents
REGULATORY ELEMENTS OF THE LEUKAEMIA INHIBITORY FACTOR (LIF) PROMOTER IN MURINE BONE MARROW STROMAL CELLS
1999
Leukaemia inhibitory factor (LIF) plays an important role as a haematopoietically active cytokine. As described earlier in a murine model, interleukin 1 (IL-1) induced LIF mRNA and protein expression. We utilized the murine cell line +/+-1.LDA11 to further define regulatory mechanisms of LIF expression in bone marrow stromal cells. The production of LIF mRNA is stimulated by IL-1beta, TNF-alpha, and the cAMP analogue 8-bromoadenosine 3':5'-monophosphate (8BrcAMP). LIF mRNA expression is controlled at the transcriptional level. Different fragments from -542 to -45 bp 5' upstream of the transcriptional start site of the murine LIF gene were fused to the luciferase gene. All LIF-promoter lucif…
The Insect Mushroom Bodies: a Paradigm of Neural Reuse
2013
This paper is devoted to discuss the implementation of models,which are inspired by the fly Drosophila melanogaster and able to handle open problems in the field of robotics such as attention, expectation and sequence learning. The role of the Mushroom Bodies (MBs) in solving these tasks is analyzed in detail and a unifying plausible biologically inspired model is proposed. The developed neural structure is able to show different capabilities in line with the paradigm of neural reuse. The same neural circuit can be exploited to accomplish multiple tasks showing interesting capabilities such as attention, expectation and delayed match-to-sample. The simulation results here reported suggest a…
Solving the Problems of Inspection Planning under Parametric Uncertainty of Underlying Models
2013
Certain fatigued structures must be inspected in order to detect fatigue damages that would otherwise not be apparent. A technique for obtaining optimal inspection strategies is proposed for situations where it is difficult to quantify the costs associated with inspections and undetected failure. For fatigued structures for which failures (fatigue damages) are only detected at the time of inspection, it is important to be able to determine the optimal times of inspection. Fewer inspections will lead to lower fatigue reliability of the structure upon demand, and frequent inspection will lead to higher cost. When there is a fatigue reliability requirement, the problem is usually to develop an…
The Neural Basis of Idea Density During Natural Spoken Language
2019
Idea density (ID) evolved as a quantification of propositional base structure. Besides its function as a measure of linguistic complexity, ID has also been used as an index of general linguistic ability. In order to find the neural basis for the processing of high or low ID during spontaneous speech, a sample of healthy adults was assessed using the functional resonance imaging (fMRI) technique; participants described pictures presented to them while in the scanner. Differential patterns of activation were observed for the low- and high-ID conditions, providing new insights into the processing correlates of ID.
Decision Making and Optimization for Inspection Planning under Parametric Uncertainty of Underlying Models
2013
Certain fatigued structures must be inspected in order to detect fatigue damages that would otherwise not be apparent. A technique for obtaining optimal inspection strategies is proposed for situations where it is difficult to quantify the costs associated with inspections and undetected failure. For fatigued structures, for which failures (fatigue damages) are only detected at the time of inspection, it is important to be able to determine the optimal times of inspection. Fewer inspections will lead to lower fatigue reliability of the structure upon demand, and frequent inspections will lead to higher cost. When there is a fatigue reliability requirement, the problem is usually to develop …
An 18S rDNA-Based Molecular Phylogeny of Aphidiinae (Hymenoptera: Braconidae)
2000
We have obtained a molecular phylogeny of the subfamily Aphidiinae (Hymenoptera: Braconidae) by sequencing the 18S rDNA in 37 aphidiine taxa. Approximately 1857 nucleotides were sequenced in each species. Evolutionary relationships were established by comparing the results of maximum-parsimony, maximum-likelihood, and distance analyses. The most variable region of this gene, V4 (approx 403 nucleotides), was employed to establish the basality of the tribe Ephedrini within this subfamily. All phylogenetic reconstructions yielded trees with very similar topologies that confirmed the existence of two of the four traditionally accepted tribes, Ephedrini and Praini, but questioned the existence o…
Evolutionary relationships among the members of an ancient class of non-LTR retrotransposons found in the nematode Caenorhabditis elegans.
1998
We took advantage of the massive amount of sequence information generated by the Caenorhabditis elegans genome project to perform a comprehensive analysis of a group of over 100 related sequences that has allowed us to describe two new C. elegans non-LTR retrotransposons. We named them Sam and Frodo. We also determined that several highly divergent subfamilies of both elements exist in C. elegans. It is likely that several master copies have been active at the same time in C. elegans, although only a few copies of both Sam and Frodo have characteristics that are compatible with them being active today. We discuss whether it is more appropriate under these circumstances to define only 2 elem…
The complete genome sequence of Lamium mild mosaic virus, a member of the genus Fabavirus
2013
Springer-Verlag Wien 2013 Abstract Lamium mild mosaic virus (LMMV) is the only one of the five members of the genus Fabavirus for which there are no nucleotide sequence data. In this study, the complete genome sequence of LMMV was determined and compared with the available complete genome sequences of other members of the genus Fabavirus. The genome was the largest of the genus but maintained the typical orga- nization, with RNA 1 of 6080 nucleotides (nt), RNA 2 of 4065 nt, and an unusually long 3 0 untranslated region in RNA 2 of 603 nt. Phylogenetic analysis of the amino acid sequences of the protease-polymerase (Pro-Pol) region and the two coat proteins confirmed that LMMV belongs to a d…
Molecular evolution of the metazoan protein kinase C multigene family
1996
Protein kinases C (PKCs) comprise closely related Ser/Thr kinases, ubiquitously present in animal tissues ; they respond to second messengers, e.g., Ca2+ and/or diacylglycerol, to express their activities. Two PKCs have been sequenced from Geodia cydonium, a member of the lowest multicellular animals, the sponges (Porifera). One sponge G. cydonium PKC, GCPKC1, belongs to the ''novel'' (Ca2+-independent) PKC (nPKC) subfamily while the second one, GCPKC2, has the hall-marks of the ''conventional'' (Ca2+-dependent) PKC (cPKC) subfamily. The alignment of the Ser/Thr catalytic kinase domains, of the predicted aa sequences for these cDNAs with respective segments from previously reported sequence…
Evolution of the Secondary Symbiont “Candidatus Serratia symbiotica” in Aphid Species of the Subfamily Lachninae ▿†
2008
ABSTRACT Buchnera aphidicola BCc, the primary endosymbiont of the aphid Cinara cedri (subfamily Lachninae), is losing its symbiotic capacity and might be replaced by the coresident “ Candidatus Serratia symbiotica.” Phylogenetic and morphological analyses within the subfamily Lachninae indicate two different “ Ca . Serratia symbiotica” lineages and support the longtime coevolution of both symbionts in C. cedri .