Molecular evolution of the metazoan protein kinase C multigene family

6533b834fe1ef96bd129cbcc

RESEARCH PRODUCT

Molecular evolution of the metazoan protein kinase C multigene family

Helena ĆEtković Werner E. G. M�ller Michael Kruse Vera Gamulin Zeev Pancer Isabel M. Müller

subject

Subfamily Molecular Sequence Data Protein Serine-Threonine Kinases Homology (biology)Catalysis Evolution Molecular Genetics Animals sponges ; Geodia cydonium ; serine/threonine kinases ; phylogeny ; molecular systematics ; molecular evolution Amino Acid Sequence Molecular Biology Ecology Evolution Behavior and Systematics Protein kinase C Phylogeny Protein Kinase C Genetics Protein-Serine-Threonine Kinases biology Base Sequence Sequence Homology Amino Acid Kinase Cyclin-dependent kinase 2 PKCS Cell biology Porifera enzymes and coenzymes (carbohydrates)Protein kinase domain Multigene Family biology.protein biological phenomena cell phenomena and immunity

description

Protein kinases C (PKCs) comprise closely related Ser/Thr kinases, ubiquitously present in animal tissues ; they respond to second messengers, e.g., Ca2+ and/or diacylglycerol, to express their activities. Two PKCs have been sequenced from Geodia cydonium, a member of the lowest multicellular animals, the sponges (Porifera). One sponge G. cydonium PKC, GCPKC1, belongs to the ''novel'' (Ca2+-independent) PKC (nPKC) subfamily while the second one, GCPKC2, has the hall-marks of the ''conventional'' (Ca2+-dependent) PKC (cPKC) subfamily. The alignment of the Ser/Thr catalytic kinase domains, of the predicted aa sequences for these cDNAs with respective segments from previously reported sequences, revealed highest homology to PKCs from animals but also distant relationships to Ser/Thr kinases from protozoa, plants, and bacteria. However, a comparison of the complete structures of the sponge PKCs, which are-already-identical to those of nPKCs and cPKCs from higher metazoa, with the structures of protozoan, plant, and bacterial Ser/Thr kinases indicates that the metazoan PKCs have to be distinguished from the nonmetazoan enzymes. These data indicate that metazoan PKCs have a universal common ancestor which they share with the nonmetazoan Ser/Thr kinases with respect to the kinase domain, but they differ from them in overall structural composition.

year	journal	country	edition	language
1996-10-01

10.1007/bf02339011 https://www.bib.irb.hr/39419