Search results for "sequence"

Aperiodicity in finitely generated bi-ideals and bounded bi-ideals

2014

Disertācijā pētīts aperiodiskums divu vienpusēji bezgalīgu vārdu klašu – galīgi ģenerētu biideālu un ierobežotu bi-ideālu – kontekstā. Gan galīgi ģenerētus bi-ideālus, gan ierobežotus biideālus ir ērti uzdot, izmantojot bāzes virkni. Ierobežotu bi-ideālu gadījumā tiek nofiksēta galīga vārdu kopa, no kuras tiek uzģenerēta bāzes virkne. Savukārt galīgi ģenerētu bi-ideālu gadījumā bāzes virkne ir periodiska, līdz ar to var uzskatīt, ka ir dota galīga bāze. Efektīvā ģenerēšana un zināmie aperiodiskuma nosacījumi kalpo par motivāciju apskatīt galīgi ģenerētu bi-ideālu potenciālo pielietojumu kriptogrāfijā – aperiodisku gadījuma skaitļu ģenerēšanā. Savukārt, ierobežoti bi-ideāli ir galīgi ģenerēt…

Gaia-ESO Survey: Global properties of clusters Trumpler 14 and 16 in the Carina nebula

2017

Aims: We present the first extensive spectroscopic study of the global population in star clusters Trumpler 16, Trumpler 14, and Collinder 232 in the Carina nebula, using data from the Gaia-ESO Survey, down to solar-mass stars. Methods: In addition to the standard homogeneous survey data reduction, a special processing was applied here because of the bright nebulosity surrounding Carina stars. Results: We find about 400 good candidate members ranging from OB types down to slightly subsolar masses. About 100 heavily reddened early-type Carina members found here were previously unrecognized or poorly classified, including two candidate O stars and several candidate Herbig Ae/Be stars. Their l…

The Gaia-ESO Survey: Structural and dynamical properties of the young cluster Chamaeleon i

2017

Investigating the physical mechanisms driving the dynamical evolution of young star clusters is fundamental to our understanding of the star formation process and the properties of the Galactic field stars. The young (~2 Myr) and partially embedded cluster Chamaeleon I is one of the closest laboratories for the study of the early stages of star cluster dynamics in a low-density environment. The aim of this work is to study the structural and kinematical properties of this cluster combining parameters from the high-resolution spectroscopic observations of the Gaia-ESO Survey with data from the literature. Our main result is the evidence of a large discrepancy between the velocity dispersion …

Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C

2020

Macroautophagy is a conserved degradative process for maintaining cellular homeostasis and plays a key role in aging and various human disorders. The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C. The molecular function, regulation and cellular localization of LC3A and LC3C have not been investigated frequently, even if a similar function to that described for LC3B appears likely. Here, we have selectively decapacitated LC3B by three separate strategies in primary human fibroblasts and analyzed the evoked e…

Exploring evolutionary responses to increasing temperature in an environmental opportunistic pathogen

2017

A (1->3)-beta-D-glucan recognition protein from the sponge Suberites domuncula. Mediated activation of fibrinogen-like protein and epidermal growth f…

2004

Sponges (phylum Porifera) live in a symbiotic relationship with microorganisms, primarily bacteria. Until now, molecular proof for the capacity of sponges to recognize fungi in the surrounding aqueous milieu has not been available. Here we demonstrate, for the demosponge Suberites domuncula (Porifera, Demospongiae, Hadromerida), a cell surface receptor that recognizes (1--3)-beta-D-glucans, e.g. curdlan or laminarin. This receptor, the (1--3)-beta-D-glucan-binding protein, was identified and its cDNA analysed. The gene coding for the 45 kDa protein was found to be upregulated in tissue after incubation with carbohydrate. Simultaneously with the increased expression of this gene, two further…

Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade

2014

A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of…

Anchorage of Candida albicans Ssr1 to the cell wall, and transcript profiling of the null mutant.

2005

Incorporation into the wall of Candida albicans Ssr1, a GPI-dependent protein, was investigated by construction of different truncated genes for which the three potential omega sites (S199, S215 and G216) and the corresponding omega+1 and omega+2 were eliminated or modified. Cells of the C. albicans ssr1Delta mutant were transformed with pADH-pl harboring the truncated versions of CaSSR1, pADH-DeltaCaSSR1t(217-234) (lacking a C-terminal hydrophobic stretch of 18 aa including the putative omega+2 and omega+1, omega+2 of S215 and G216) or pADH-DeltaCaSSR1t(199-201) (lacking three serine residues), and their walls were analyzed for the protein. Results suggested that the three serine residues …

Pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (Dicentrarchus labrax) fed functional diets.

2014

The routine use of chemotherapy to control bacterial diseases in aquatic populations has resulted in the development and spread of antibiotic resistance. The inclusion of immunostimulants in fish diets (functional diets) is one of the main strategies to solve this threat. This study aimed to analyse the intestinal microbiota of cultured European sea bass (Dicentrarchus labrax) fed two functional diets applying pyrosequencing of PCR-amplified 16S rRNA gene. Quality-filtered reads were assigned to family and genus taxonomic levels using the Ribosomal Database Project classifier. The autochthonous intestinal microbiota of sea bass consisted of two dominant bacterial genera: Dysgonomonas (Bacte…

Phylogeny of snapdragon species (Antirrhinum; Scrophulariaceae) using non-coding cpDNA sequences

2005

Antirrhinum is an Old World genus of up to 25 perennial taxa, mainly located in the western Mediterranean basin. A molecular analysis of 24 taxa of Antirrhinum was undertaken using cpDNA sequences from the trnT (UGU)-trnL (UAA) 5' exon region. The Kimura two-parameter model was chosen to calculate pairwise nucleotide divergence values between cpDNA sequences, and a bootstrapped neighbor-joining dendrogram was constructed from the nucleotide divergence distance matrix. Eighteen sites were variable across the studied samples and the position of 7 indels, ranging from 1 to 7 bp, was inferred from the sequence alignment. Several trnT-trnL sequences are identical in: some members of subsection K…