Search results for "sequence"
showing 10 items of 4987 documents
A global DNA repair mechanism involving the Cockayne syndrome B (CSB) gene product can prevent the in vivo accumulation of endogenous oxidative DNA b…
2002
The Cockayne syndrome B (CSB) gene product is involved in the repair of various types of base modifications in actively transcribed DNA sequences. To investigate its significance for the repair of endogenous oxidative DNA damage, homozygous csb(-/-)/ogg1(-/-) double knockout mice were generated. These combine the deficiency of CSB with that of OGG1, a gene coding for the mammalian repair glycosylase that initiates the base excision repair of 7,8-dihydro-8-oxoguanine (8-oxoG). Compared to ogg1(-/-) mice, csb(-/-)/ogg1(-/-) mice were found to accumulate with age severalfold higher levels of oxidited purine modifications in hepatocytes, splenocytes and kidney cells. In contrast, the basal (ste…
Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate
2007
Abstract Background The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited. Results Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of …
mRNA expression profiles of primary high-grade central osteosarcoma are preserved in cell lines and xenografts
2011
Abstract Background Conventional high-grade osteosarcoma is a primary malignant bone tumor, which is most prevalent in adolescence. Survival rates of osteosarcoma patients have not improved significantly in the last 25 years. Aiming to increase this survival rate, a variety of model systems are used to study osteosarcomagenesis and to test new therapeutic agents. Such model systems are typically generated from an osteosarcoma primary tumor, but undergo many changes due to culturing or interactions with a different host species, which may result in differences in gene expression between primary tumor cells, and tumor cells from the model system. We aimed to investigate whether gene expressio…
Parathyroid hormone-related peptide and 8701-BC breast cancer cell growth and invasion in vitro: evidence for growth-inhibiting and invasion-promotin…
1995
It has been previously reported that 8701-BC cells, derived from a primary carcinoma of the breast, constitutively express parathyroid hormone-related peptide (PTHrP) gene and that N-terminal PTHrP immunoreactivity can be found in cell medium. Here we have firstly measured immunoreactive PTHrP in 8701-BC cell medium using antibodies raised against midregion and C-terminal fragments, and also demonstrated the expression of PTH/PTHrP receptor by 8701-BC cells. Secondly, we have examined the role, if any, elicited by diverse PTHrP domains on 8701-BC cell proliferation, and invasive behaviour in vitro related to production of extracellular proteolytic enzymes. Our data show that PTHrP [1-34], a…
Niedożywienie szpitalne i jego następstwa
2015
Odżywianie jest jedną z podstawowych funkcji organizmu. Brak pokarmu doprowadza do śmierci tak samo jak brak tlenu lub wody, lecz trwa znacznie wolniej. Przedłużające się głodzenie lub nadmierne nasilenie procesów metabolicznych może doprowadzić do stanu niedożywienia. Charakteryzuje się ono obniżeniem odporności oraz zwiększoną podatnością na choroby, powoduje także wzrost umieralności. Niedożywienie jest obecnie problemem zdrowia publicznego w krajach rozwiniętych i rozwijających się. Niedożywienie szpitalne jest zjawiskiem powszechnym, sprzyja występowaniu powikłań, wydłuża hospitalizację i zwiększa koszty leczenia. Następstwa niedożywienia dotyczą wszystkich narządów i układów, także sf…
Machine Learning Models for Measuring Syntax Complexity of English Text
2019
In this paper we propose a methodology to assess the syntax complexity of a sentence representing it as sequence of parts-of-speech and comparing Recurrent Neural Networks and Support Vector Machine. We have carried out experiments in English language which are compared with previous results obtained for the Italian one.
AB Dor A VLBI images
2020
The fast rotator, pre-main sequence star AB Dor A is a strong and persistent radio emitter. The extraordinary coronal flaring activity is thought to be the origin of compact radio emission and other associated phenomena, such as large slingshot prominences. We aim to investigate the radio emission mechanism and the milliarcsecond radio structure around AB Dor A. We performed phase-referenced VLBI observations at 22.3GHz, 8.4GHz, and 1.4GHz over more than one decade using the Australian VLBI array. Our 8.4GHz images show a double core-halo morphology, similar at all epochs, with emission extending at heights between 5 and 18 stellar radii. Furthermore, the sequence of the 8.4GHz maps shows a…
Late Quaternary sedimentary evolution of the Castellammare Gulf (North-Western Sicily offshore)
2005
High-resolution seismic stratigraphy has been applied to the interpretation of the stratigraphic architecture of Late Pleistocene and Holocene successions on the continental shelf and upper slope of the Gulf of Castellammare, North-Western Sicily. The Late Quaternary type 1 depositional sequence is bounded by a regional unconformity and it is made up of four systems tracts, developed under fourth order relative sea level fluctuations, after the previous highstand of isotope Stage 5e. Systems tract are located in different parts of the basin, two being confined to the outer shelf and the upper slope, the other developed along the inner shelf. Facies associations, geomorphological elements, a…
Heterogeneity within and between primary colorectal carcinomas and matched metastases as revealed by analysis of Ki-ras and p53 mutations
2004
Analysis of the genetic status of Ki-ras and p53 in primary colorectal carcinomas and matched colorectal liver metastasis from 30 patients reveals an overall heterogeneity both within and between the two tumoral tissues. Both genes were found mutated with a similar frequency in both tissues; however, identical mutations in primary tumor and matched metastasis were found less frequently in the case of the Ki-ras than the p53 gene. Only in three cases the same p53 and Ki-ras mutations found in the primary tumor were found also in the metastasis. In several metastatic specimens the DNA bearing a mutation detected also in the primary tumor appears significantly less abundant than the wild-type …
Evidence for Positive Selection in the Capsid Protein-Coding Region of the Foot-and-Mouth Disease Virus (FMDV) Subjected to Experimental Passage Regi…
2001
We present sequence data from two genomic regions of foot-and-mouth disease virus (FMDV) subjected to several experimental passage regimens. Maximum-likelihood estimates of the nonsynonymous-to-synonymous rate ratio parameter (dN/dS) suggested the action of positive selection on some antigenic sites of the FMDV capsid during some experimental passages. These antigenic sites showed an accumulation of convergent amino acid replacements during massive serial cytolytic passages and also in persistent infections of FMDV in cell culture. This accumulation was most significant at the antigenic site A (the G-H loop of capsid VP1), which includes an Arg-Gly-Asp (RGD) cellular recognition motif. Our …