Search results for "shr"
showing 10 items of 455 documents
Eutrophic status influences the impact of pesticide mixtures and predation on Daphnia pulex populations
2021
Abstract Pesticides, nutrients, and ecological stressors such as competition or predation co‐occur in freshwater ecosystems impacted by agriculture. The extent to which combinations of these stressors affect aquatic populations and the role of nutrients availability in modulating these responses requires further understanding. In this study, we assessed how pesticides affecting different taxonomic groups and predation influence the response of Daphnia pulex populations under different trophic conditions. An outdoor experiment was designed following a factorial design, with the insecticide chlorpyrifos, the herbicide diuron, and the predation by Notonecta sp. individuals as key stressors. Th…
Energy and economic analysis of air-to-air heat pumps as an alternative to domestic gas boiler heating systems in the South of Italy
2019
Abstract According to the electricity tariff recently issued by the Italian government for the domestic heating systems fed with heat pumps (HP), an energy and economic analysis was carried out with the aim of investigating the effectiveness of the air-to-air heat pump systems (HPS) as an alternative to the traditional heating using domestic gas boilers systems (GBS). For the purpose of this study, the apartments of three residential buildings were examined. The hourly heating loads were calculated with the ASHRAE radiant time series and the coefficient of performance (COP) of the HPs was evaluated for each operating condition. The costs for the heating system installation and electricity a…
The impact of the volumetric swelling behavior on the water uptake of gas shale
2018
Abstract Water uptake of gas shales is commonly considered one of the most important factors responsible for fluid loss during flowback operations after hydraulic fracturing. Imbibition experiments cover a key role in this context to analyze the impact of several factors (such as mineralogical composition, fluids composition, anisotropic structure, volumetric swelling) that contribute to the water uptake in these unconventional reservoirs. The aim of this study is the quantification of the impact of the volumetric response (swelling and shrinkage) of gas shales on the water uptake during imbibition and desiccation processes. An experimental methodology to quantify the volumetric response du…
Structural characterization of polysaccharides of a productive strain of the culinary-medicinal king oyster mushroom, pleurotus eryngii (Agaricomycet…
2018
A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H, 13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear singlequantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating uni…
Gene Expression and Apoptosis Levels in Cumulus Cells of Patients with Polymorphisms of FSHR and LHB Undergoing in Vitro Fertilization Program
2017
Background/Aims: FSH receptor (FSHR) Ala307Thr and Asn680Ser and LHβ chain (LHB) Trp28Arg and Ile35Thr polymorphisms affect the response to pharmacological ovarian stimulation with r-FSH in women undergoing assisted reproductive treatment (ART). Here, we evaluated the expression level of selected genes involved in follicle maturation and the possible onset of apoptosis in cumulus cells of patients with single and double FSHR and LHB polymorphisms, as potential markers of oocyte competence. Methods: Cumulus cells from 36 stimulated patients were collected and SNP genotyping performed by PCR. Gene expression was evaluated through real-time PCR, and apoptosis estimated via TUNEL assay, and cle…
Biological investigation of neural circuits in the insect brain
2018
Watching insects thoughtfully one cannot but adore their behavioural capabilities. They have developed amazing reproductive, foraging and orientation strategies and at the same time they followed the evolutionary path of miniaturization and sparseness. Both features together turn them into a role model for autonomous robots. Despite their tiny brains, fruit flies (Drosophila) can orient, walk on uneven terrain, in any orientation to gravity, can fly in adverse winds, find partners, places for egg laying, food and shelter. Drosophila melanogaster is the model animal for geneticists and cutting-edge tools are being continuously developed to study the underpinnings of their behavioural capabil…
Motor-skill learning in an insect inspired neuro-computational control system
2017
In nature, insects show impressive adaptation and learning capabilities. The proposed computational model takes inspiration from specific structures of the insect brain: after proposing key hypotheses on the direct involvement of the mushroom bodies (MBs) and on their neural organization, we developed a new architecture for motor learning to be applied in insect-like walking robots. The proposed model is a nonlinear control system based on spiking neurons. MBs are modeled as a nonlinear recurrent spiking neural network (SNN) with novel characteristics, able to memorize time evolutions of key parameters of the neural motor controller, so that existing motor primitives can be improved. The ad…
Retinal homeobox promotes cell growth, proliferation and survival of mushroom body neuroblasts in the Drosophila brain.
2016
Abstract The Drosophila mushroom bodies, centers of olfactory learning and memory in the fly ‘forebrain’, develop from a set of neural stem cells (neuroblasts) that generate a large number of Kenyon cells (KCs) during sustained cell divisions from embryonic to late pupal stage. We show that retinal homeobox ( rx ), encoding for an evolutionarily conserved transcription factor, is required for proper development of the mushroom bodies. Throughout development rx is expressed in mushroom body neuroblasts (MBNBs), their ganglion mother cells (MB-GMCs) and young KCs. In the absence of rx function, MBNBs form correctly but exhibit a reduction in cell size and mitotic activity, whereas overexpress…
Forager age and foraging state, but not cumulative foraging activity, affect biogenic amine receptor gene expression in the honeybee mushroom bodies
2020
Foraging behavior is crucial for the development of a honeybee colony. Biogenic amines are key mediators of learning and the transition from in-hive tasks to foraging. Foragers vary considerably in their behavior, but whether and how this behavioral diversity depends on biogenic amines is not yet well understood. For example, forager age, cumulative foraging activity or foraging state may all be linked to biogenic amine signaling. Furthermore, expression levels may fluctuate depending on daytime. We tested if these intrinsic and extrinsic factors are linked to biogenic amine signaling by quantifying the expression of octopamine, dopamine and tyramine receptor genes in the mushroom bodies, i…
The Amino-Terminal Domain of GRK5 Inhibits Cardiac Hypertrophy through the Regulation of Calcium-Calmodulin Dependent Transcription Factors.
2018
We have recently demonstrated that the amino-terminal domain of G protein coupled receptor kinase (GRK) type 5, (GRK5-NT) inhibits NFκB activity in cardiac cells leading to a significant amelioration of LVH. Since GRK5-NT is known to bind calmodulin, this study aimed to evaluate the functional role of GRK5-NT in the regulation of calcium-calmodulin-dependent transcription factors. We found that the overexpression of GRK5-NT in cardiomyoblasts significantly reduced the activation and the nuclear translocation of NFAT and its cofactor GATA-4 in response to phenylephrine (PE). These results were confirmed in vivo in spontaneously hypertensive rats (SHR), in which intramyocardial adenovirus-med…