Search results for "simplex"

Asymptomatic vaginal herpes simplex virus infections in mice: virology and pathohistology

1996

One of the causes of genital tract infections in humans are herpes simplex virus types 1 and 2 (HSV-1, HSV-2). Although primary and recurrent infections can be clinically apparent and in part very serious, many infections are asymptomatic and result only in temporary genital shedding of virus (recurrences). During our investigations of vaginitis, strain IES of HSV-1 produced an asymptomatic infection. Replication in the murine vaginal (vag.) epithelium as well as antibody formation after vag. infection was comparable to those of survivors after infection with highly virulent strains. Titration of liver, spleen, ovaries, adrenal glands spinal cord, or brain after vag. IES infection revealed …

Human genetic polymorphisms and risk of viral infection after solid organ transplantation.

2021

The immune system plays a key role in the host defense against viral pathogens. A signaling cascade is activated upon infection involving a variety of molecules such as pattern-recognition receptors (PRRs), interleukins or antiviral interferons. Long-term immunosuppression after solid organ transplantation (SOT) mainly abrogates adaptive T-cell-mediated responses, thus highlighting the relative contribution of innate immunity. Single-nucleotide polymorphisms (SNPs) within genes coding for PRRs or soluble mediators have been associated with differential susceptibility to viral infections among SOT recipients. A protective effect against cytomegalovirus (CMV) infection or disease has been att…

Influence of double infections on the induction of thymidine kinase by UV-irradiated herpes simplex virus types 1 and 2 and pseudorabies virus

1975

Simultaneous infection of primary rabbit kidney cells with HSV type 1 TK+ and a TK- strain results in a mutual influence of both viruses on the induction of thymidine kinase (TK). TK+ virus has an enhancing and TK- virus a depressing effect on TK induction by a superinfecting TK+ virus. The enzyme induction depends on the ratio of multiplicities of both viruses. The mutual influence on TK induction depends further on the time of addition of the superinfecting virus: the effect of the second virus can still be observed when given 6 hours after primary infection. Identical phenomena can be observed using combinations with HSV type 2 or Pseudorabies viruses. The ability of HSV to induce TK is …

Construct validity of the Moral Development Scale for Professionals (MDSP)

2011

Olle Söderhamn1,2, John Olav Bjørnestad1, Anne Skisland1, Christina Cliffordson21Faculty of Health and Sport Sciences, University of Agder, Grimstad and Kristiansand, Norway; 2Department of Nursing, Health and Culture, University West, Trollhättan, SwedenAbstract: The aim of this study was to investigate the construct validity of the Moral Development Scale for Professionals (MDSP) using structural equation modeling. The instrument is a 12-item self-report instrument, developed in the Scandinavian cultural context and based on Kohlberg’s theory. A hypothesized simplex structure model underlying the MDSP was tested through structural equation modeling. …

Comparison of a Manual and an Automated Method to Estimate the Number of Uterine Eggs in Anisakid Nematodes: To Coulter or Not to Coulter. Is That th…

2007

Studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. This situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. This note examines whether a manual method based on visual counts in aliquots and an automated method using a Coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nematode species (Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum). The number of eggs from 50 females per nematode species was estimated using both techniques. The automated and ma…

Activity investigation of pinostrobin towards herpes simplex virus-1 as determined by atomic force microscopy

2009

In the present study, the antiviral activity of pinostrobin towards herpes simplex virus-1 (HSV-1) was investigated by MTT assay and atomic force microscopy. Pinostrobin can inhibit HSV-1 replication with 50% effective concentration (EC(50)) of 22.71 ± 1.72 μg/ml. MTT assay showed HSV-1 was significantly inhibited when pretreated with pinostrobin, with the inhibition of 85.69 ± 2.59%. Significant changes in morphology and size of HSV-1 were observed by atomic force microscopy (AFM) in response to pinostrobin treatment. AFM topography and phase images showed that with increasing time, the envelope was shedded and damaged, finally leading to virus inactivation. With increasing concentration, …

OPTIMIZATIONS FOR TENSORIAL BERNSTEIN–BASED SOLVERS BY USING POLYHEDRAL BOUNDS

2010

The tensorial Bernstein basis for multivariate polynomials in n variables has a number 3n of functions for degree 2. Consequently, computing the representation of a multivariate polynomial in the tensorial Bernstein basis is an exponential time algorithm, which makes tensorial Bernstein-based solvers impractical for systems with more than n = 6 or 7 variables. This article describes a polytope (Bernstein polytope) with a number of faces, which allows to bound a sparse, multivariate polynomial expressed in the canonical basis by solving several linear programming problems. We compare the performance of a subdivision solver using domain reductions by linear programming with a solver using a c…

Planification expérimentale en agroalimentaire

2006

Relationship between HLA I surface expression and different cytopathic effects produced after herpes simplex virus infection in vitro.

1992

In the present study, we investigated the effects of herpes simplex virus (HSV) infection on the expression of HLA class I antigens and beta 2-microglobulin in human fibroblasts. The mRNA abundance for HLA class I was shown to be strongly reduced after infection with HSV strains either producing cell rounding or fusion from within (FFWI), however, HLA class I expression on the surface of cells is strongly reduced only after appearance of FFWI. Using a ts mutant (ts 78R) or CyA in combination with a fusion from without (FFWO) inducing strain of HSV, this loss of HLA class I antigens is assumed to be correlated to the rearrangement of the cell membrane during the fusion process itself as a la…

GASTROINTESTINAL HELMINTHS OF THE DUSKY DOLPHIN, LAGENORHYNCHUS OBSCURUS (GRAY, 1828), OFF PATAGONIA, IN THE SOUTHWESTERN ATLANTIC

1999

The stomachs and intestines of 23 dusky dolphins incidentally caught in a trawl fishery off Patagonia were surveyed for helminths. All the dolphins were parasitized, with a total of 3,936 helminth individuals. Only five species occurred, of which three were common (prevalence ± 10%): Anisakis simplex, Braunina cordiformis and Hadwenius sp. A. simplex was present in all the dolphins and showed the highest abundance and mean intensity (104.9 individuals/infected host). B. cordiformis was next most common (87%), and Hadwenius sp. third (52.2%). A. simplex was found mostly in the stomach (94.8%), B. cordiformis in the duodenal ampulla (51.4%), and Hadwenius sp. in the stomach (64.5%). The rare …