Search results for "solu"
showing 10 items of 7577 documents
Transcutol containing vesicles for topical delivery of minoxidil
2010
The aim of this work was to evaluate the ability of Transcutol (Trc) to produce elastic vesicles with soy lecithin (SL) and study the influence of the obtained vesicles on in vitro (trans)dermal delivery of minoxidil. To this purpose, so-called penetration enhancer-containing vesicles (PEVs) were prepared using Trc aqueous solutions (5-10-20-30% v/v) as hydrophilic phase. SL liposomes, without Trc, were used as control. Prepared formulations were characterized in terms of size distribution, morphology, zeta potential, deformability, and rheological behavior. The influence of the obtained PEVs on (trans)dermal delivery of minoxidil was studied by in vitro diffusion experiments through pig sk…
Solubilized liver extracellular matrix maintains primary rat hepatocyte phenotype in-vitro.
2015
Whole organ engineering and cell-based regenerative medicine approaches are being investigated as potential therapeutic options for end-stage liver failure. However, a major challenge of these strategies is the loss of hepatic specific function after hepatocytes are removed from their native microenvironment. The objective of the present study was to determine if solubilized liver extracellular matrix (ECM), when used as a media supplement, can better maintain hepatocyte phenotype compared to type I collagen alone or solubilized ECM harvested from a non-liver tissue source. Liver extracellular matrix (LECM) from four different species was isolated via liver tissue decellularization, solubil…
Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging.
2010
Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The pr…
Synthetic aperture superresolution with multiple off-axis holograms
2006
An optical setup to achieve superresolution in microscopy using holographic recording is presented. The technique is based on off-axis illumination of the object and a simple optical image processing stage after the imaging system for the interferometric recording process. The superresolution effect can be obtained either in one step by combining a spatial multiplexing process and an incoherent addition of different holograms or it can be implemented sequentially. Each hologram holds the information of each different frequency bandpass of the object spectrum. We have optically implemented the approach for a low-numerical-aperture commercial microscope objective. The system is simple and rob…
Axial superresolution by synthetic aperture generation
2008
The use of tilted illumination onto the input object in combination with time multiplexing is a useful technique to overcome the Abbe diffraction limit in imaging systems. It is based on the generation of an expanded synthetic aperture that improves the cutoff frequency (and thus the resolution limit) of the imaging system. In this paper we present an experimental validation of the fact that the generation of a synthetic aperture improves not only the lateral resolution but also the axial one. Thus, it is possible to achieve higher optical sectioning of three-dimensional (3D) objects than that defined by the theoretical resolution limit imposed by diffraction. Experimental results are provi…
Holography and Superresolution
2013
The capability of improving the spatial resolution of imaging systems is usually known as superresolution. Some methods provide improve resolution by playing with the imaging part of the system and without modifying the optical parameters of the imaging lenses. And others act over the geometry, shape and size of sampling pixels in the detection array. The former strategy allows optical superresolution while the latter provide geometrical superresolution. In this contribution, we will review the state of the art in optical superresolution approaches understood as the possibility to overcome the limited resolving power of imaging systems beyond the bounds imposed by Abbe's diffraction theory.…
Coupling SAR X-band and optical data for NDVI retrieval: model calibration and validation on two test areas
2013
Sustainability of modern agro-hydrology requires the knowledge of spatial and temporal variability of vegetation biomass to optimize management of land and water resources. Diversely from optical imaging, temporal resolution of active sensors, such as SAR, is not limited by sky cloudiness; thus, they may be combined with optical imageries to provide a more continuous monitoring of land surfaces. Several new SAR missions (e.g., ALOS-PALSAR, COSMO-SkyMed 1 and 2, TerraSAR-X, TerraSAR-X2, Sentinel 1) acquiring at X-, C- and L-bands and dual polarization capability, are characterized by a short revisit time (from 12 h to ~10 days) and high spatial resolution (<20 m). These satellites could prov…
Vegetation index retrieval by coupling optical and SAR images
2012
Monitoring spatial and temporal variability of Vegetation Indices (VIs) is important to manage land and water resources, with significant impact on the sustainability of modern agriculture Although algorithms based on optical data give accurate products, cloud cover dramatically reduces the temporal resolution of these outputs. The launch of new Synthetic Aperture Radar (SAR) constellations such as COSMO-Skymed opened new opportunities to develop agro-hydrological applications. Indeed, these satellites may represent a suitable source of data for operational applications due to their high spatial and temporal resolutions (10 m in StripMap PingPong acquisition mode, best revisit time with 4 s…
Investigating the Relationship between X-Band SAR Data from COSMO-SkyMed Satellite and NDVI for LAI Detection
2013
Monitoring spatial and temporal variability of vegetation is important to manage land and water resources, with significant impact on the sustainability of modern agriculture. Cloud cover noticeably reduces the temporal resolution of retrievals based on optical data. COSMO-SkyMed (the new Italian Synthetic Aperture RADAR-SAR) opened new opportunities to develop agro-hydrological applications. Indeed, it represents a valuable source of data for operational use, due to the high spatial and temporal resolutions. Although X-band is not the most suitable to model agricultural and hydrological processes, an assessment of vegetation development can be achieved combing optical vegetation indices (V…
Surpassing digital holography limits by lensless object scanning holography.
2012
We present lensless object scanning holography (LOSH) as a fully lensless method, capable of improving image quality in reflective digital Fourier holography, by means of an extremely simplified experimental setup. LOSH is based on the recording and digital post-processing of a set of digital lensless holograms and results in a synthetic image with improved resolution, field of view (FOV), signal-to-noise ratio (SNR), and depth of field (DOF). The superresolution (SR) effect arises from the generation of a synthetic aperture (SA) based on the linear movement of the inspected object. The same scanning principle enlarges the object FOV. SNR enhancement is achieved by speckle suppression and c…