Search results for "solution"

Pulsed electric field and pH assisted selective extraction of intracellular components from microalgae Nannochloropsis

2015

Abstract The study was aimed at investigation of the potential of pulsed electric field (PEF) pre-treatment as a preliminary step of pH-assisted aqueous extraction of algae components from microalgae Nannochloropsis suspensions. The PEF and sonication (S) were compared as pretreatment methods. They were applied at normal (pH = 8.5) and basic (pH = 11) conditions, and supplementary basic extraction (at pH = 11) was done. The extracts were analyzed for content of pigments, proteins, carbohydrates, total phenolic compounds and antioxidant capacity. The colloidal stability of PEF- and S-pretreated suspensions was also evaluated. The data evidence that PEF technique allows selective extraction o…

Does plant diversity influence phosphorus cycling in experimental grasslands?

2011

Plant diversity was shown to influence the N cycle, but plant diversity effects on other nutrients remain unclear. We tested whether plant species richness or the presence/absence of particular functional plant groups influences P partitioning among differently extractable pools in soil, P concentrations in soil solution, and exploitation of P resources (i.e. the proportion of total bioavailable P in plants and soil that was stored in aboveground biomass) by the plant community in a 5-year biodiversity experiment in grassland.The experimental grassland site established in 2002 had 82 plots with different combinations of numbers of species (1, 2, 4, 8, 16, 60) and functional groups (grasses,…

Comparison of input data with different spatial resolution in landscape pattern analysis – A case study from northern Latvia

2017

A suitable spatial scale needs to be selected in geographical and landscape ecological research, and this requires great consideration as different scales have profound effect on derived landscape spatial patterns. Numerous studies have investigated the effects of different scales on landscape metrics using simulated patterns, but few have been conducted to compare different data sources with variable scale for regional- and landscape-scale assessments. Possibly this has occurred because researchers have been prone to use the best available source, a well-known standard, and easiest to use. This study was conducted to assess the impact of input data resolution on values of landscape pattern…

Downscaling hydrodynamics features to depict causes of major productivity of Sicilian-Maltese area and implications for resource management

2018

Abstract Chlorophyll- a ( CHL-a ) and sea surface temperature ( SST ) are generally accepted as proxies for water quality. They can be easily retrieved in a quasi-near real time mode through satellite remote sensing and, as such, they provide an overview of the water quality on a synoptic scale in open waters. Their distributions evolve in space and time in response to local and remote forcing, such as winds and currents, which however have much finer temporal and spatial scales than those resolvable by satellites in spite of recent advances in satellite remote-sensing techniques. Satellite data are often characterized by a moderate temporal resolution to adequately catch the actual sub-gri…

Fundamental questions and applications of sclerochronology: Community-defined research priorities

2020

WOS:000582677500029; International audience; Horizon scanning is an increasingly common strategy to identify key research needs and frame future agendas in science. Here, we present the results of the first such exercise for the field of sclerochronology, thereby providing an overview of persistent and emergent research questions that should be addressed by future studies. Through online correspondence following the 5th International Sclerochronology Conference in 2019, participants submitted and rated questions that addressed either knowledge gaps or promising applications of sclerochronology. An initial list of 130 questions was compiled based on contributions of conference attendees and …

2020

Abstract The filamentous fungus Neurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructed N. crassa strains that contain a modified csr-1 locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for…

Marked Neurospora crassa strains for competition experiments and Bayesian methods for fitness estimates

2019

AbstractThe filamentous fungusNeurospora crassa, a model microbial eukaryote, has a life cycle with many features that make it suitable for studying experimental evolution. However, it has lacked a general tool for estimating relative fitness of different strains in competition experiments. To remedy this need, we constructedN. crassastrains that contain a modifiedcsr-1locus and developed an assay for detecting the proportion of the marked strain using a post PCR high resolution melting assay. DNA extraction from spore samples can be performed on 96-well plates, followed by a PCR step, which allows many samples to be processed with ease. Furthermore, we suggest a Bayesian approach for estim…

Profiling Suspensions in Natural Water by a Simplified Dynamic Light Scattering Procedure and Sedimentation

2015

Abstract A coherent light scattering experiment was carried out. The samples were aqueous natural water suspensions picked from the same river. While sedimentation occurred in the samples, they were subjected to a dynamic light scattering (DLS) experiment and the time series was recorded at certain time intervals. For each recording, a program written for this purpose, performing at least square minimisation, computed the average diameter of the particles in suspension. The variation of the average diameter in time indicates the dominant type of suspensions in water.

Transformation of agricultural landscapes in the Anthropocene: Nature's contributions to people, agriculture and food security

2020

Fil: Vanbergen, Adam J. Université de Bourgogne Franche-Comté. AgroSup Dijon. Agroécologie. Francia. Fil: Aizen, Marcelo A. Universidad Nacional del Comahue. Instituto de Investigaciones en Biodiversidad y Medioambiente. Rio Negro, Argentina. Fil: Aizen, Marcelo A. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Investigaciones en Biodiversidad y Medioambiente. Rio Negro, Argentina. Fil: Cordeau, Stephane. Université de Bourgogne Franche-Comté. AgroSup Dijon. Agroécologie. Francia. Fil: Garibaldi, Lucas A. Universidad Nacional de Río Negro. Instituto de Investigaciones en Recursos Naturales, Agroecología y Desarrollo Rural. Río Negro, Argentina. Fil: Ga…

Glycymeris pilosa (Bivalvia) - A high-potential geochemical archive of the environmental variability in the Adriatic Sea.

2019

Due to its outstanding longevity (decades), the shallow-water bivalve Glycmeris pilosa represents a prime target for sclerochronological research in the Mediterranean Sea. In the present study, we analyzed the microgrowth patterns and the stable carbon (δ13Cshell) and oxygen (δ18Oshell) isotopes of the outer shell layer of live-collected G. pilosa specimens from four different sites along the Croatian coast, middle Adriatic Sea. Combined analysis of shell growth patterns and temporally aligned δ18Oshell data indicated that the main growing season lasts from April to December, with fastest growth rates occurring during July and August when seawater temperatures exceeded 22 °C. Slow growth in…