Search results for "sorting"

A multi-centre clinico-genetic analysis of the VPS35 gene in Parkinson disease indicates reduced penetrance for disease-associated variants

2012

Abstract: Background Two recent studies identified a mutation (p.Asp620Asn) in the vacuolar protein sorting 35 gene as a cause for an autosomal dominant form of Parkinson disease. Although additional missense variants were described, their pathogenic role yet remains inconclusive. Methods and results We performed the largest multi-center study to ascertain the frequency and pathogenicity of the reported vacuolar protein sorting 35 gene variants in more than 15,000 individuals worldwide. p.Asp620Asn was detected in 5 familial and 2 sporadic PD cases and not in healthy controls, p.Leu774Met in 6 cases and 1 control, p.Gly51Ser in 3 cases and 2 controls. Overall analyses did not reveal any sig…

INTACT vs. FANS for Cell-Type-Specific Nuclei Sorting: A Comprehensive Qualitative and Quantitative Comparison

2021

Increasing numbers of studies seek to characterize the different cellular sub-populations present in mammalian tissues. The techniques “Isolation of Nuclei Tagged in Specific Cell Types” (INTACT) or “Fluorescence-Activated Nuclei Sorting” (FANS) are frequently used for isolating nuclei of specific cellular subtypes. These nuclei are then used for molecular characterization of the cellular sub-populations. Despite the increasing popularity of both techniques, little is known about their isolation efficiency, advantages, and disadvantages or downstream molecular effects. In our study, we compared the physical and molecular attributes of sfGFP+ nuclei isolated by the two methods—INTACT and FAN…

An impaired peroxisomal targeting sequence leading to an unusual bicompartmental distribution of cytosolic epoxide hydrolase

1991

AbstractTo gain an understanding of the mechanism by which the subcellular distribution of cytosolic epoxide hydrolase (cEH) is directed, we have analyzed the carboxy terminal region of rat liver cEH by means of cDNA cloning to define the structure of its possible peroxisomal targeting sequence (PTS). Purified cEH was subjected to peptide analysis following endoproteinase Glu-C digestion and HPLC-separation of the fragments. The obtained sequence information was used to perform PCR experiments resulting in the isolation of a 680 bp cDNA clone encoding the carboxy terminus of cEH. The deduced amino acid sequence displays a terminal tripeptide Ser-Lys-Ile which is highly homologous to the PTS…

Immunogenomic identification and characterization of granulocytic myeloid-derived suppressor cells in multiple myeloma

2020

Granulocytic myeloid-derived suppressor cells (G-MDSCs) promote tumor growth and immunosuppression in multiple myeloma (MM). However, their phenotype is not well established for accurate monitoring or clinical translation. We aimed to provide the phenotypic profile of G-MDSCs based on their prognostic significance in MM, immunosuppressive potential, and molecular program. The preestablished phenotype of G-MDSCs was evaluated in bone marrow samples from controls and MM patients using multidimensional flow cytometry; surprisingly, we found that CD11b+CD14−CD15+CD33+HLADR− cells overlapped with common eosinophils and neutrophils, which were not expanded in MM patients. Therefore, we relied on …

What is the validity of the sorting task for describing beers? A study using trained and untrained assessors

2008

In the sensory evaluation literature, it has been suggested that sorting tasks followed by a description of the groups of products can be used by consumers to describe products, but a closer look at this literature suggests that this claim needs to be evaluated. In this paper, we proposed to examine the validity of the sorting task to describe products by trained and untrained assessors. The experiment reported here consisted in two parts. In a first part, participants sorted nine commercial beers and then described each group with their own words or with a list of terms. In a second part, participants were asked to match each beer with one of their own sets of descriptors. The matching tas…

Smart multi-carrier energy system: Optimised energy management and investment analysis

2016

This paper proposes an optimised Energy Management System for a multi-carrier hub, which integrates two energy distribution networks, for hydrogen and electricity. The economic sustainability of a real-life instantiation of such a system has been analysed as well. The Energy Management System has been developed by means of a multi-objective optimisation algorithm, the Non-dominated Sorting Genetic Algorithm II, implemented using MATLAB®. The achieved results consist in a series of set-points defining the working conditions of the plant for a chosen time horizon. Data provided by this process also show the effectiveness of the adopted optimisation approach. The financial analysis is performe…

Stochastic Approach for Optimal Positioning of Pumps As Turbines (PATs)

2021

A generic water system consists of a series of works that allow the collection, conveyance, storage and finally the distribution of water in quantities and qualities such as to satisfy the needs of end users. In places characterized by high altitude differences between the intake works and inhabited centres, the potential energy of the water is very high. This energy is attributable to high pressures, which could compromise the functionality of the pipelines

Non-linear optimization of track layouts in loop-sorting-systems

2013

Optimization used for enhancing geometric structures iswell known. Applying obstacles to the shape optimization problemis on the other hand not very common. It requires a fast contact search algorithmand an exact continuous formulation to solve the problem robustly. This paper focuses on combining shape optimization problemswith collision avoidance constraints by which a collision detection algorithmis presented. The presentedmethod is tested against the commercial loop-sorting-system used for sorting of medium sized items. The objective is to minimize price and footprint of the system whilemaintaining its functionality. Contact constraints are in this context important to include as variou…

Optimal Set Points Regulation of Distributed Generation Units in Micro-grids under Islanded Operation

2010

The present work studies the problem of optimizing the power production levels of dispersed generation units in islanded microgrids. The problem is intrinsically multi-objective with non linear objectives and constraints, thus the solution approach is based on evolutionary optimization and uses the Non dominated Sorting Genetic Algorithm II. The objectives are calculated based on the solution of the load flow problem. The latter problem is more complicated when in the considered system a physical node with a sufficiently large production capability is not available, because all the generation node of the systems have similar and limited generation capability. In this paper, the issue has be…

2021

One of the problems that hinder emergency in developing countries is the problem of monitoring a number of activities on inter-urban roadway networks. In the literature, the use of control points is proposed in the context of these countries in order to ensure efficient monitoring, by ensuring a good coverage while minimizing the installation costs as well as the number of accidents across these road networks. In this work, we propose an optimal deployment of these control points from several optimization methods based on some evolutionary multi-objective algorithms: the non-dominated sorting genetic algorithm-II (NSGA-II); the multi-objective particle swarm optimization (MOPSO); the streng…