Search results for "southern"
showing 10 items of 415 documents
Expression of C1q, a subcomponent of the rat complement system, is dramatically enhanced in brains of rats with either Borna disease or experimental …
1995
In situ hybridization, RT-PCR and Northern blot analysis as well immunohistochemistry were used to examine the expression of C1q, a subcomponent of the rat complement system, in brains of rats infected with Borna disease virus (BDV) and rats afflicted with experimental allergic encephalomyelitis (EAE) induced by the adoptive transfer of myelin basic protein specific T cells. C1q mRNA, which was not detected in normal brain, became clearly detectable using RT-PCR analysis by d14 post infection (p.i.) with BDV. Maximal levels of C1q mRNA were reached 21 days p.i. when inflammatory reactions in the brain were also at a peak. Similarly, C1q mRNA was elevated when the clinical symptoms of EAE be…
Mitochondrial and chromosomal DNA alterations in human chromophobe renal cell carcinomas
1992
Renal cell tumours are characterized by the loss of chromosome 3p and trisomy of 5q segments (common, non-papillary renal cell carcinoma), or by trisomy of chromosomes 7 and 17 and loss of the Y chromosome (papillary renal cell carcinoma), or by random karyotype changes and mitochondrial DNA alterations (renal oncocytoma). We have studied by means of RFLP analysis the genomic and mitochondrial DNA in 11 chromophobe renal cell carcinomas, which have a unique morphology among kidney cancers. We found a loss of the constitutional heterozygosity at chromosomal regions 3p, 5q, 17p, and 17q, a combination of allelic losses that has not been found in other types of renal cell tumours. Three of the…
Ulota larrainii (Orthotrichoideae, Orthotrichaceae, Bryophyta) a new species from Chile, with comments on the worldwide diversification of the genus
2015
A new species of Ulota D. Mohr, U. larrainii, is described from the Aysén Region, southern Chile. The characters that discriminate the new moss are the prostrate habit, leaves not crisped, marginal cells of the leaf base differentiated in 1(2) rows, vaginula hairy, exostome of 8 pairs of orange, recurved teeth, endostome of 16 broad and hyaline segments, and large, multicellular spores. The new moss is illustrated and compared with similar taxa. In addition, the presence of multicellular spores in the genus Ulota is summarized and evaluated from an updated list of currently accepted species. Finally, the distribution, species richness and levels of endemism in the different regions where Ul…
Detection of a Single Gene Encoding Glutamine Synthetase in Sinapis alba (L.)
1991
Summary Ion-exchange chromatography of glutamine synthetase polypeptides (GS; EC 6.3.1.2) from green leaves and the roots of Sinapis alba yielded identical elution patterns. This is likewise true for GS from etiolated cotyledons. As we have previously demonstrated the identity of GS-enzymes from etiolated and green leaf tissues, the obviously very similar charge properties of GS-proteins indicate the eventual existence of only one type of GS in all mustard plant organs. To further prove this possibility, Southern blot analysis of mustard DNA was carried out using hybridization probes specific to different GS-isoforms. Concluding from the relative strength of the hybridization signals, the G…
Planktonic foraminifera as proxies of the Holocene climatic variability (Tyrrhenian, Mediterranean Sea)
2017
Introduction. The focus of this study is the paleoclimatic reconstruction of the southern Tyrrhenian between ~9.2 and 2.9 ka, through the study of planktonic foraminiferal assemblages and stable isotopes, and comparing data with other coeval intervals. Several authors have studied the climatic sensitivity of Holocene planktonic foraminifera in different parts of the Mediterranean. Planktonic foraminifera produce good records of Holocene climatic variability, especially as regards the suborbital events such as Bond events (Bond et al., 1997) and other cooling/warming oscillations. Therefore, the obtained eco-biostratigraphy has allowed us to analyze how climatic forcing influenced sea surfac…
Physiological,anatomical and biomass partitioning responses to ozone in the Mediterranean endemic plant Lamottea dianae
2011
Ozone effects on the perennial forb Lamottea dianae were studied in an open-top chamber experiment. Ozone was found to induce reductions in CO 2 assimilation and water use efficiency in the leaves of this species. These reductions were mainly related to a decline in the in vivo CO 2 fixation capacity of Rubisco (V c,max), rather than to stomatal limitations or photoinhibitory damage (F v:F m). In addition to chloroplast degeneration, other observed effects were callose accumulation, formation of pectinaceous wart-like cell wall exudates and phloem alterations. Moreover, ozone exposure significantly reduced root dry biomass. The possible relevance of these adverse effects for Mediterranean f…
A revision of medium and small sized deer from the Middle and Late Pleistocene of Calabria and Sicily
2001
The remains of Megaceroides calabriae from Middle Pleistocene deposits at locality Bovetto (Southern Calabria) and of Megaceroides carburangelensis from the late Middle and Late Pleistocene locality Cimillà (South-eastern Sicily) are revised. These deer were considered as endemic species of strongly reduced size, related to genus Megaceroides (=Praemegaceros), which evolved in insular environment similarly to the endemic megaceroids from Sardinia, Corsica and Crete, M, cazioti and M. cretensis. The comparison of M. calabriae and M. carburangelensis with representatives of genera Megaceroides and Dama, carried out in the present study, shows that the Calabrian and Sicilian deer belong to Dam…
International student mobility in Southern-Latin Europe: beyond the EU logics, towards a new space
2018
This paper discusses international student mobility (ISM) in Southern-Latin Europe, specifically Italy, Portugal, and Spain, analysing the inflow of international students as reflected in the UNESCO, OECD and European Commission databases. Only recently Italy, Portugal and Spain, as latecomers, have become more actively involved in ISM dynamics. This trend has been a response to EU pressures to internationalization, instrumented through the consolidation of the Bologna process and the need to build a common space of higher education. The analysis shows that at the intra-European level Italy, Portugal and Spain share similar ISM patterns; however, in the global context other logics shape ISM…
Molecular genetics of familial hypercholesterolemia in Spain: Ten novel LDLR mutations and population analysis
2001
Mutations underlying FH in Spain are largely unknown because only a few and limited surveys have been carried out on Spanish FH patients up to now. To gain information on this issue, we have analysed a group of 113 unrelated Spanish FH patients from an eastern area of Spain (Valencian Community). We have screened the LDLR gene by Southern blot and PCR-SSCP analysis to detect large rearrangements and small mutations, respectively. In addition, we have screened the Apo B gene for mutations known to cause FDB by PCR-SSCP analysis. We have identified a total of 47 different mutations in the LDLR gene (5 large rearrangements, and 42 small mutations, which were characterized by DNA sequencing), 1…
Axolotl MHC architecture and polymorphism.
1999
The MHC of the urodele amphibian Ambystoma mexicanum consists of multiple polymorphic class I loci linked, so far as yet known, to a single class II B locus. This architecture is very different from that of the anuran amphibian Xenopus. The number of class I loci in the axolotl can vary from 6 to 21 according to the haplotypes as shown by cDNA analysis and Southern blot studies in families. These loci can be classified into seven sequence groups with features ranging from the class Ia to the class Ib type. All individuals express genes from at least three of the seven groups, and all individuals possess the class Ia-like type.