Search results for "spectrophotometric"
showing 10 items of 14 documents
Ferric-bipyridine assay: A novel spectrophotometric method for measurement of antioxidant capacity
2020
Measurement of the antioxidant potential using in vitro assays is paramount in the assessment of various food products and nutraceuticals. Researchers always attempt to develop more accurate assays which can be performed in unsophisticated conditions. This novel method, Ferric-Bipyridine reducing capacity of total antioxidants (FBRC) is a very simple, accurate assay performed based on the reduction of Fe (III) to Fe (II) by antioxidants with the formation of a colored complex with bipyridine (Bp) i.e, Fe(II)-Bp. The FBRC method thus developed was assessed under carefully adjusted parameters of oxidant concentration, pH, temperature, solvent, light and time in order to fix the optimum condit…
A Comprehensive Spectroscopic Analysis of the Ibuprofen Binding with Human Serum Albumin, Part I
2020
Human serum albumin (HSA) plays a fundamental role in the human body. It takes part in the transport of exogenic and endogenic substances, especially drugs. Ibuprofen (IBU) is one of the most commonly used non-steroidal anti-inflammatory drugs, used for pain relief, fever relief, and for anti-inflammatory purposes. The binding of ligands with HSA is a significant factor which determines the toxicity and the therapeutic dosages of these substances. The aim of this study was to compare the degree of ibuprofen binding with human serum albumin at various temperatures and protein solution pH values. In order to evaluate conformational changes in HSA caused by interaction with ibuprofen, spectrop…
Mosaic floors of roman Villa del Casale: Principal component analysis on spectrophotometric and colorimetric data
2013
Abstract Spectrophotometric and colorimetric data obtained during a measurement campaign aimed at supporting the Roman "Villa del Casale" (Piazza Armerina, Sicily, Italy) conservation activities, are presented. Special attention was paid to the possible variation of the chromatic coordinates, possibly due to the interventions of cleaning, consolidation, and protection. Data have been analyzed by the Principal Component Analysis (PCA) statistical technique, with the attempt to investigate its role in data variability reduction and verify its effectiveness in interpreting the phenomena occurring on the mosaic surface of the Villa, through grouping the observations into homogenous clusters. Ef…
Experimental conditions for respiration and growth studies of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax
2022
Water parameters in the 2 years before spawning of F0 (08.02.2016-06.03.2018) and during larval and juvenile phase of F1: Larval period until 17.05.2018 (48 dph, 900 dd) and 01.06.2018 (63 dph, ~900 dd) for warm and cold life condition respectively, for the juveniles until 28.09.2018 (180 dph, ~4000 dd) and 12.02.2019 (319 dph, ~5100 dd) for warm and cold conditioned fish respectively. Means ± s.e. over all replicate tanks per condition. Temperature (Temp.), pH (free scale), salinity, oxygen and total alkalinity (TA) were measured weekly in F1 and monthly in F0; sea water (SW) measurements were conducted in 2017 and 2018. Water parameters during larval and early juvenile phase of F0: Larval…
Determination of cholesterol in human milk: an alternative to chromatographic methods
2015
Introduction: human milk (HM) is considered the best option for feeding healthy infants. Cholesterol (CHOL) is important for proper development of the nervous system, and for hormone and vitamin synthesis in growing infants. Breastfeeding and dietary CHOL intake during infancy have been suggested to affect blood lipid levels and the risk of cardiovascular disease in adulthood. Gas chromatography is the technique most widely used to determine CHOL in HM. Chromatographic methods are specific for the determination of CHOL and other sterols present in HM, but are extremely time consuming, and the costs and equipment requirements mean that they are not accessible to all laboratories. Aim: the pr…
A Comprehensive Spectroscopic Analysis of the Ibuprofen Binding with Human Serum Albumin, Part II
2021
Human serum albumin (HSA) is the most abundant human plasma protein. HSA plays a crucial role in many binding endos- and exogenous substances, which affects their pharmacological effect. The innovative aspect of the study is not only the interaction of fatted (HSA) and defatted (dHSA) human serum albumin with ibuprofen (IBU), but the analysis of the influence of temperature on the structural modifications of albumin and the interaction between the drug and proteins from the temperature characteristic of near hypothermia (308 K) to the temperature reflecting inflammation in the body (312 K and 314 K). Ibuprofen is a non-steroidal anti-inflammatory drug. IBU is used to relieve acute pain, inf…
Micellization properties of cardanol as a renewable co-surfactant
2015
With the aim to improve the features of surfactant solutions in terms of sustainability and renewability we propose the use of hydrogenated natural and sustainable plant-derived cardanol as an additive to com- mercial surfactants. In the present study we demonstrated that its addition, in amounts as high as 10%, to commercial surfactants of different charge does not significantly affect surfactant properties. Conversely, the presence of hydrogenated cardanol can strongly affect spectrophotometric determination of CMC if preferential interactions with the dyes used take place. This latter evidence may be profitably exploited in surfactant manufacturing by considering that the concurrent pres…
Selective and Sensitive Chromofluorogenic Detection of the Sulfite Anion in Water Using Hydrophobic Hybrid Organic-Inorganic Silica Nanoparticles
2013
[EN] In water and wine: Chromofluorogenic detection of the sulfite anion in pure water was accomplished by using a new hybrid organic-inorganic material that contained a probe entrapped in hydrophobic biomimetic cavities. This material was used for the detection of sulfite in red wine. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Structural investigations on diorganotin and triorganotin(IV) phosphomycin derivatives.
2008
Abstract Four new diorganotin(IV), (R = Me, Bu), and triorganotin(IV), (R = Me, Ph), derivatives of the phosphomycin disodium salt antibiotic[(1R,2S)-1,2-epoxypropylphosphonate]Na2 have been synthesized and their solid state configuration studied by X-ray crystallography, FT-IR, Mossbauer, UV–Vis spectroscopies. The X-ray diffraction investigation, performed on the bis[trimethyltin(IV)]phosphomycin, showed that the coordination geometry at all the Sn atoms is trigonal bipyramidal. The structure of the complex forms an unusual polymeric zig-zag planar network. The FT-IR and the 119Sn Mossbauer studies supported the formation of trigonal bipyramidal (Tbp) molecular structures, both in the dio…
Determinación del colesterol en leche humana: una alternativa a los métodos cromatográficos
2015
Introduction: human milk (HM) is considered the best option for feeding healthy infants. Cholesterol (CHOL) is important for proper development of the nervous system, and for hormone and vitamin synthesis in growing infants. Breastfeeding and dietary CHOL intake during infancy have been suggested to affect blood lipid levels and the risk of cardiovascular disease in adulthood. Gas chromatography is the technique most widely used to determine CHOL in HM. Chromatographic methods are specific for the determination of CHOL and other sterols present in HM, but are extremely time consuming, and the costs and equipment requirements mean that they are not accessible to all laboratories. Aim: the pr…