6533b883fe1ef96bd12dc0dd

RESEARCH PRODUCT

Experimental conditions for respiration and growth studies of F0 and F1 larval and juvenile European seabass Dicentrarchus labrax

Sarah HowaldMarta MoyanoAmélie CrespelLuis L KuchenmüllerLouise CominassiGuy ClaireauxMyron A PeckFelix Christopher Mark

subject

Calculated by CO2sys_xls_program Lewis and WallaceSalinityAlkalinityLife stageType of studyTemperature waterGermanyCalculatedAlkalinity totalSalinity standard errortotallarval growthteleostexperiment2006pH2007 with purified m cresol purpleOcean acidificationMeasured spectrophotometrically Dickson et alTemperatureWeilheimPartial pressure of carbon dioxide (water) at sea surface temperature (wet air) standard errorSilicatedissolvedLaboratory experimentSEAL AA3 segmented flow autoanalyzerSilicate standard errorstandard errorMultiprobeEarth System ResearchWTW 340imetabolic rateswaterGenerationOxygen dissolvedMultiprobe WTW 340iPhosphateocean warmingAlkalinity total standard errorjuvenile growthCarbon dioxide (water) partial pressurepH meter (WTW 3110) with electrode (WTW Sentix 41)DATE TIMEWTW Oxi 340i probeTemperature water standard errorCarbon dioxide water partial pressurepH standard errorXylem Analytics GermanySalinometer (WTW LF325 Xylem Analytics Germany Weilheim Germany)Calculated by CO2sys_xls_program (Lewis and Wallace 2006)OxygenTreatmentDATE/TIMEPartial pressure of carbon dioxide water at sea surface temperature wet airSalinometer WTW LF325Oxygen dissolved standard errorMeasured spectrophotometrically (Dickson et al. 2007) with purified m-cresol purplepH meter WTW 3110 with electrode WTW Sentix 41Phosphate standard error

description

Water parameters in the 2 years before spawning of F0 (08.02.2016-06.03.2018) and during larval and juvenile phase of F1: Larval period until 17.05.2018 (48 dph, 900 dd) and 01.06.2018 (63 dph, ~900 dd) for warm and cold life condition respectively, for the juveniles until 28.09.2018 (180 dph, ~4000 dd) and 12.02.2019 (319 dph, ~5100 dd) for warm and cold conditioned fish respectively. Means ± s.e. over all replicate tanks per condition. Temperature (Temp.), pH (free scale), salinity, oxygen and total alkalinity (TA) were measured weekly in F1 and monthly in F0; sea water (SW) measurements were conducted in 2017 and 2018. Water parameters during larval and early juvenile phase of F0: Larval period until (45 dph, 900 dd, 06.12.2013), early juveniles until 1.5 years. Means ± s.e.m. over all measurements per condition (triplicate tanks in larvae, single tanks in juveniles). Temperature (Temp.) and pH (NBS scale) were measured daily. pH (total scale), salinity, phosphate, silicate and total alkalinity (TA) were measured once at the beginning and once at the end of the larval phase and 9 times during juvenile phase; PCO2 was calculated with CO2sys; A–Ambient PCO2, D1000 –ambient + 1000 µatm CO2, L – Larvae, J – Juveniles.

https://doi.org10.1594/pangaea.941766https://doi.org/https://doi.org/10.1594/PANGAEA.941766