Search results for "sport."

Processus d’agglomération et définition de la ville

1997

International audience

Sport Ethics: The substantialist proposal of Lumpkin, Stoll and Beller, from ethical proceduralism

2013

En este artículo presento de forma crítica la propuesta de ética del deporte como «bolsa de virtudes» que elaboran tres autores estadounidenses que son Lumpkin, Stoll y Beller. Dicha propuesta debe entenderse como una manifestación de sustancialismo neoaristotélico en deporte, con una clara influencia de MacIntyre. Mi objetivo es mostrar cómo dicho sustancialismo no es suficiente para una propuesta ética del deporte, por lo que abogo por incorporar algunos de los presupuestos del procedimentalismo ético aplicado al deporte. In this article the author presents critically the proposed sports ethics as «bag of virtues» that Americans made three authors are Lumpkin, Stoll and Beller. This propo…

Peptide-mediated interference with baculovirus transduction

2007

Baculovirus represents a multifunctional platform with potential for biomedical applications including disease therapies. The importance of F3, a tumor-homing peptide, in baculovirus transduction was previously recognized by the ability of F3 to augment viral binding and gene delivery to human cancer cells following display on the viral envelope. Here, F3 was utilized as a molecular tool to expand understanding of the poorly characterized baculovirus-mammalian cell interactions. Baculovirus-mediated transduction of HepG2 hepatocarcinoma cells was strongly inhibited by coincubating the virus with synthetic F3 or following incorporation of F3 into viral nucleocapsid by genetic engineering, th…

Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane

2021

Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …

Assembly and Translocation of Papillomavirus Capsid Proteins

2002

ABSTRACT The major and minor capsid proteins of polyomavirus are preassembled in the cytoplasm and translocated to the nucleus only as a VP1-VP2/VP3 complex. In this study, we describe independent nuclear translocation of the L1 major protein and the L2 minor capsid protein of human papillomavirus type 33 by several approaches. First, we observed that expression and nuclear translocation of L2 in natural lesions precede expression of L1. Second, using a cell culture system for coexpression, we found that accumulation of L2 in nuclear domain 10 (ND10) subnuclear structures precedes L1 by several hours. In contrast, complexes of L2 and mutants of L1 forced to assemble in the cytoplasm are tra…

Nuclear localization but not PML protein is required for incorporation of the papillomavirus minor capsid protein L2 into virus-like particles.

2004

ABSTRACT Recent reports suggest that nuclear domain(s) 10 (ND10) is the site of papillomavirus morphogenesis. The viral genome replicates in or close to ND10. In addition, the minor capsid protein, L2, accumulates in these subnuclear structures and recruits the major capsid protein, L1. We have now used cell lines deficient for promyelocytic leukemia (PML) protein, the main structural component of ND10, to study the role of this nuclear protein for L2 incorporation into virus-like particles (VLPs). L2 expressed in PML protein knockout (PML −/− ) cells accumulated in nuclear dots, which resemble L2 aggregates forming at ND10 in PML protein-containing cells. These L2 assemblies also attracted…

Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.

1992

Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.

Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry

2012

ABSTRACT Adeno-associated virus type 2 (AAV2) has gained much interest as a gene delivery vector. A hallmark of AAV2-mediated gene transfer is an intracellular conformational change of the virus capsid, leading to the exposure of infection-relevant protein domains. These protein domains, which are located on the N-terminal portion of the structural proteins VP1 and VP2, include a catalytic phospholipase A 2 domain and three clusters of basic amino acids. We have identified additional protein sequence motifs located on the VP1/2 N terminus that also proved to be obligatory for virus infectivity. These motifs include signals that are known to be involved in protein interaction, endosomal sort…

Ultrasound-guided viscosupplementation in glenohumeral microinstability in sports athletes

2014

Izturības īpašību attīstīšana futbolā pusaudžiem

2022

Futbolistu pusaudžu treniņu procesam un metodiskajiem paņēmieniem ir jābūt tik kvalitatīviem, lai tie sekmētu un attīstītu fizisko īpašību izturību. Ir daudzas metodes kā var trenēt izturības veidus. Kvalifikācijas darba tēma ir “Izturības īpašību attīstīšana futbolā pusaudžiem”. Kvalifikācijas darba mērķis ir- noskaidrot Madonas BJSS MT-6 futbola pusaudžu treniņgrupas izturības trenētību. Izstrādājot kvalifikācijas darbu, tika izmantotas sekojošas pētīšanas metodes- sporta treniņu teorijas, metodikas, sporta fizioloģijas, biomehānikas literatūras avotu pētīšana un analīze; aptaujas metode- intervija; pedagoģiskais novērojums; kvalifikācijas darba autors tika veicis kontrolvingrinājumus izt…