Search results for "structural"

showing 10 items of 5047 documents

High-affinity and selective detection of pyrophosphate in water by a resorcinarene salt receptor

2017

N-Alkyl ammonium resorcinarenes selectively bind pyrophosphate in pure water with an exceptionally high binding constant of up to 1.60 × 107 M–1, three orders of magnitude higher than ATP.

pyrophosphatereceptors010402 general chemistryMass spectrometry01 natural sciencesPyrophosphateChloridemolecular diagnosticschemistry.chemical_compoundmedicineresorcinarenesta116Biochemistry Biophysics and Structural Biologyta114010405 organic chemistryIsothermal titration calorimetryGeneral ChemistryResorcinarenePhosphateCombinatorial chemistryOrders of magnitude (mass)0104 chemical sciencesChemistrychemistrySelectivitymedicine.drug
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RAB18 Loss Interferes With Lipid Droplet Catabolism and Provokes Autophagy Network Adaptations

2020

Autophagy is dependent on appropriate lipid supply for autophagosome formation. The regulation of lipid acquisition and the autophagy network response to lipid-limiting conditions are mostly elusive. Here, we show that the knockout of the RAB GTPase RAB18 interferes with lipid droplet catabolism, causing an impaired fatty acid release. The resulting reduced lipid-droplet-derived lipid availability influences autophagy and provokes adaptive modifications of the autophagy network. These adjustments include increased expression and phosphorylation of ATG2B as well as augmented formation of the ATG12-ATG5 conjugate. Moreover, ATG9A shows an enhanced phosphorylation at amino acid residues tyrosi…

rab3 GTP-Binding ProteinsImmunoblottingGTPaseReal-Time Polymerase Chain Reaction03 medical and health sciences0302 clinical medicineMicroscopy Electron TransmissionStructural BiologyLipid dropletAutophagyHumansPhosphorylationTyrosineMolecular Biology030304 developmental biology0303 health sciencesMicroscopy ConfocalChemistryCatabolismAutophagyAutophagosomesLipid DropletsImmunohistochemistryCell biologyrab GTP-Binding ProteinsPhosphorylationlipids (amino acids peptides and proteins)RabCRISPR-Cas Systems030217 neurology & neurosurgeryRAB18HeLa CellsJournal of Molecular Biology
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Rac1 and PAK1 are upstream of IKK-ε and TBK-1 in the viral activation of interferon regulatory factor-3

2004

The anti-viral type I interferon (IFN) response is initiated by the immediate induction of IFN beta, which is mainly controlled by the IFN-regulatory factor-3 (IRF-3). The signaling pathways mediating viral IRF-3 activation are only poorly defined. We show that the Rho GTPase Rac1 is activated upon virus infection and controls IRF-3 phosphorylation and activity. Inhibition of Rac1 leads to reduced IFN beta promoter activity and to enhanced virus production. As a downstream mediator of Rac signaling towards IRF-3, we have identified the kinase p21-activated kinase (PAK1). Furthermore, both Rac1 and PAK1 regulate the recently described IRF-3 activators, I kappa B kinase- and TANK-binding kina…

rac1 GTP-Binding ProteinTranscription GeneticBiophysicsIκB kinaseProtein Serine-Threonine KinasesSignal transductionBiologyVirus ReplicationBiochemistryCell LineDogsPAK1Structural BiologyInterferonGeneticsmedicineAnimalsHumansPhosphorylationPromoter Regions Geneticp21-activated kinasesMolecular BiologyRNA Double-StrandedKinaseRho GTPaseI-Kappa-B KinaseNuclear ProteinsInterferon-betaCell BiologyCREB-Binding ProteinI-kappa B KinaseDNA-Binding ProteinsEnzyme Activationp21-Activated KinasesInfluenza A virusViral infectionAnti-viral responseTrans-ActivatorsCancer researchInterferon Regulatory Factor-3Transcription factorSignal transductionDimerizationTranscription FactorsInterferon regulatory factorsmedicine.drugFEBS Letters
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Fra le reti e la città. Lo spazio delle nuove stazioni per l'Alta Velocità

2014

Between networks and cities: Architectural space in the new High-Speed train stations. Over the past thirty years, megainvestment rail projects in Western Europe have become powerful transformation vectors. An extensive literature addresses the issue with an urban engineering oriented approach, concerned primarily with the large scale and focusing on governance and urban planning. This monograph section of “Trasporti & Cultura” examines the relationship of new high-speed railroad stations to their cities. The objective is to investigate the depth and meaning of the transformations being wrought by the new stations, in a comparative international scenario. Articles on Japan and China are fol…

railroad high speed station infrastructural architecture urban regenerationSettore ICAR/14 - Composizione Architettonica E Urbana
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STRUCTURAL OPTIMIZATION AND STRUCTURAL ANALYSIS OF COMPOSITE MATERIAL PANEL FOR NAVAL USE

2008

The purpose of the work is the realization of a composite material with long glass fibers having better characteristic than a fiber random composite, to permit the reduction of weight and costs to shipyards for pleasure craft. Structural optimization is performed by ANSYS for the choice of the layers disposition to obtain the maximum stiffness with minimum material employment, saving weight. The study is centered on the research of the better configurations of plies packing in relation of pure shear stress for four different plies. Unidirectional plies, both symmetric orthotropic and symmetric non-orthotropic ones, are realized successively by the vacuum bag technique. Experimental tests of…

random composite composite material test structural optimization
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Biological and Proteomic Characterization of the Anti-Cancer Potency of Aqueous Extracts from Cell-Free Coelomic Fluid of Arbacia lixula Sea Urchin i…

2022

Echinoderms are an acknowledged source of bioactive compounds exerting various beneficial effects on human health. Here, we examined the potential in vitro anti-hepatocarcinoma effects of aqueous extracts of the cell-free coelomic fluid obtained from the sea urchin Arbacia lixula using the HepG2 cell line as a model system. This was accomplished by employing a combination of colorimetric, microscopic and flow cytometric assays to determine cell viability, cell cycle distribution, the possible onset of apoptosis, the accumulation rate of acidic vesicular organelles, mitochondrial polarization, cell redox state and cell locomotory ability. The obtained data show that exposed HepG2 cells under…

reactive oxygen specieSettore CHIM/10 - Chimica Degli AlimentiSettore BIO/05 - ZoologiaHepG2 cellOcean Engineeringapoptosisea urchinechinodermmitochondrial transmembrane potentialcell cycleacidic vesicular organelleSettore BIO/06 - Anatomia Comparata E Citologiacoelomic fluidcoelomic fluid; sea urchin; echinoderm; HepG2 cells; apoptosis; cell cycle; acidic vesicular organelles; mitochondrial transmembrane potential; reactive oxygen species; wound healing assaywound healing assayWater Science and TechnologyCivil and Structural EngineeringJournal of Marine Science and Engineering
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The proposal to transform an old limestone quarry into a botanical garden with a rainforest zone: a case study

2019

Nowadays, a significant part of cities is tackling the problems with post-mining areas. This manuscript is an original research which shows possibilities of their reclamation. The aim of the article is to present the proposal of developing the closed limestone quarry and creating a botanical garden. The proposed spatial solutions allow for creating a new, tourist and recreation space, maintaining the natural heritage. The work also assumed carrying out a dendrological inventory, in order to determine the existing dendrofl ora. The required spatial, nature and communication analyses, which illustrate the current condition of the area and defi ne further design works, have also been carried o…

reclamationAgroforestrysustainability planningecological restorationBiodiversityBuilding and ConstructionRainforestGeotechnical Engineering and Engineering GeologyEngineering (General). Civil engineering (General)Environmental technology. Sanitary engineeringBiodiversity conservationGeographygreen infrastructureLand reclamationBotanical gardenbiodiversity conservationTA1-2040Green infrastructureRestoration ecologyTD1-1066Earth-Surface ProcessesGeneral Environmental ScienceCivil and Structural EngineeringbiodiversityPrzegląd Naukowy Inżynieria i Kształtowanie Środowiska
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Influence of brand image of a sports event on the recommendation of its participants

2020

The aim of this research is to test the psychometric properties and relationships of a model where, from brand congruence of a sport event, trust and commitment to it can be generated, influencing future behavior of participants and resulting in a higher recommendation also called Word of Mouth (hereinafter WOM). A quantitative approach has been carried out by means of a validated questionnaire with Likert-type responses, and data were analyzed with the EQS 6.4 software. The findings indicated that the model is adequate, and also confirm that from congruence, we can explain, at a theoretical level, 45% of trust, and in the same way, from the congruence and trust, we can explain 67% of the c…

recommendationGeography Planning and Developmentlcsh:TJ807-830lcsh:Renewable energy sourcesWord of mouthManagement Monitoring Policy and Lawstructural equation modelingStructural equation modelingBrand imageCongruence (geometry)0502 economics and businessbrandsports eventslcsh:Environmental scienceslcsh:GE1-350EsportsRenewable Energy Sustainability and the Environmentlcsh:Environmental effects of industries and plants05 social sciencescommitmentbrand congruenceWOMtrustValidated questionnairelcsh:TD194-195sports managementmarketing050211 marketingEducació físicaPsychologySport managementSocial psychology050203 business & management
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On the degree of binder activity of reclaimed asphalt and degree of blending with recycling agents

2020

It is common practice to assume full blending of the aged binder of reclaimed asphalt (RA) within the design of new asphalt mixtures. Although being practical, this assumption has often led designers to asphalt mixtures lacking binder. Hence, going towards recycled asphalt mixtures (RAM) there is a need to have a better understanding of the blending phenomena, to have a general agreement on terminology and finally adapting RA classification and mix design procedures accordingly. This manuscript aims at being both a reference and stimulus for the scientific community to work in this direction and on this basis provides a nomenclature and a theoretical framework of the blending phenomena. The…

recycling agent050210 logistics & transportationdegree of blendingWaste managementdegree of activity05 social sciences0211 other engineering and technologies02 engineering and technologyrecycling agent blending phenomenablending phenomena; degree of activity; degree of availability; degree of blending; recycled asphalt; recycling agentblending phenomenaDegree (temperature)degree of availabilityAsphaltrecycled asphalt021105 building & construction0502 economics and businessCivil and Structural EngineeringMathematics
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Methodical redesign of a semitrailer

2005

redesign semitrailer composite material structural floor
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