Search results for "technique"
showing 10 items of 5596 documents
Improving the conservation of Mediterranean Chondrichthyans : the ELASMOMED DNA barcode reference library
2017
Cartilaginous fish are particularly vulnerable to anthropogenic stressors and environmental change because of their K-selected reproductive strategy. Accurate data from scientific surveys and landings are essential to assess conservation status and to develop robust protection and management plans. Currently available data are often incomplete or incorrect as a result of inaccurate species identifications, due to a high level of morphological stasis, especially among closely related taxa. Moreover, several diagnostic characters clearly visible in adult specimens are less evident in juveniles. Here we present results generated by the ELASMOMED Consortium, a regional network aiming to sample …
Involvement of putative glutamate receptors in plant defence signaling and NO production
2011
International audience; Ionotropic glutamate receptors (iGluRs) are non-selective cation channels permeable to calcium, present in animals and plants. In mammals, glutamate is a well-known neurotransmitter and recently has been recognized as an immunomodulator. As animals and plants share common mechanisms that govern innate immunity with calcium playing a key role in plant defence activation, we have checked the involvement of putative iGluRs in plant defence signaling. Using tobacco cells, we first provide evidence supporting the activity of iGluRs as calcium channels and their involvement in NO production as reported in animals. Thereafter, iGluRs were shown to be activated in response t…
Reverse-engineering the Arabidopsis thaliana transcriptional network under changing environmental conditions
2009
46 pages, 4 tables, 6 figures, 3 additinoal files.
Evidence for ontogenetically and morphologically distinct alternative reproductive tactics in the invasive Round Goby Neogobius melanostomus.
2017
Alternative reproductive tactics are characterized by the occurrence of discrete alternative morphs that differ in behavioural, morphological and physiological traits within the same sex. Although much effort has been made to describe the behaviour, morphology and physiology of such alternative morphs, less effort has been invested investigating how much overlap there is in the characteristics of such morphs in natural populations. We studied random population samples of the invasive Round Goby Neogobius melanostomus from five different localities in the river Rhine system in the Netherlands. We found two morphologically and physiologically distinct male morphs which likely represent altern…
The effects of data collection and observation methods on uncertainty of social networks in wild primates
2020
International audience; In social species, network centralities of group members shape social transmission and other social phenomena. Different factors have been found to influence the measurement of social networks, such as data collection and observation methods. In this study, we collected data on adults and juveniles and examined the effect of data collection method (ad libitum sampling vs. focal animal sampling) and observation method (interaction—grooming; play—vs. association—arm‐length; 2 m; 5 m proximities—) on social networks in wild vervet monkeys. First, we showed using a bootstrapping method, that uncertainty of ad libitum grooming and play matrices were lesser than uncertaint…
Squamation and ecology of thelodonts
2017
Thelodonts are an enigmatic group of Paleozoic jawless vertebrates that have been well studied from taxonomical, biostratigraphic and paleogeographic points of view, although our knowledge of their ecology and mode of life is still scant. Their bodies were covered by micrometric scales whose morphology, histology and the developmental process are extremely similar to those of extant sharks. Based on these similarities and on the well-recognized relationship between squamation and ecology in sharks, here we explore the ecological diversity and lifestyles of thelodonts. For this we use classic morphometrics and discriminant analysis to characterize the squamation patterns of a significant num…
Cultivating for the Industry: Cropping Experiences with Hypericum perforatum L. in a Mediterranean Environment
2021
Hypericum perforatum is an intensively studied medicinal plant, and much experimental activity has been addressed to evaluate its bio-agronomical and phytochemical features as far. In most cases, plant material used for experimental purposes is obtained from wild populations or, alternatively, from individuals grown in vases and/or pots. When Hypericum is addressed to industrial purposes, the most convenient option for achieving satisfactory amounts of plant biomass is field cultivation. Pot cultivation and open field condition, however, are likely to induce different responses on plant’s metabolism, and the obtained yield and composition are not necessarily the same. To compare these manag…
Seasonal effects on mortality rates and resprouting of stems treated with glyphosate in the invasive tree of heaven (Ailanthus altissima (Mill.) Swin…
2015
Tree of heaven (Ailanthus altissima) is regarded as invasive within urban and natural areas worldwide. Efficient methods to control it are significantly needed if we are to limit its well-known environmental and economic impacts. Up to now the use of herbicides has proven necessary since following mechanical damage, Ailanthus vigorously resprouts. However, the seasonal response of Ailanthus stems, treated with herbicides, has never been assessed. We compared the control efficacy recorded in autumn, winter and summer in an abandoned suburban citrus grove in Sicily, under Mediterranean-climate conditions. Glyphosate was injected within drill holes made in knee-high cut trees. Tree mortality a…
Grafting affects yield and phenolic profile of Solanum melongena L. landraces
2016
Abstract The influence of grafting on agronomical and qualitative characteristics of four Sicilian eggplant landraces was investigated. Grafted and ungrafted plants were compared in the open field in the northern coast of Sicily. Solanum torvum seedlings were used as rootstock. Regardless of genotypes tested, grafting significantly increased total fruit production, marketable production, and number of marketable fruits, but did not affect weight of marketable fruits and waste production. Landrace 2 (Sciacca), with black epidermal tissue and pyriform fruit shape, when grafted onto S. torvum not only gave a higher yield performance than ungrafted plants, but also showed a higher phenolic anti…
Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro
1992
Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC 2.5.1.1). This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed.