Search results for "techniques"
showing 10 items of 4426 documents
Differences between the Glycosylation Patterns of Haptoglobin Isolated from Skin Scales and Plasma of Psoriatic Patients
2012
Improved diagnosis of psoriasis, by new biomarkers, is required for evaluating the progression rate of the disease and the response to treatment. Haptoglobin (Hpt), a glycoprotein secreted by hepatocytes and other types of cells including keratinocytes, was found with glycan changes in psoriasis and other diseases. We previously reported that Hpt isolated from plasma of psoriatic patients is more fucosylated than Hpt of healthy subjects. The aim of this study was to compare the glycosylation pattern of Hpt isolated from skin scales or plasma of patients with psoriasis with that of Hpt from cornified epidermal layer or plasma of healthy subjects. High performance liquid chromatography analys…
Synthetic MUC1 Antitumor Vaccine Candidates with Varied Glycosylation Pattern Bearing R/S-configured Pam3 CysSerLys4.
2016
The Toll-like receptor 2 ligand Pam3 CysSer is of particular interest for the construction synthetic vaccines because of its ability to stimulate of the innate immune system. Such vaccines usually comprise Pam3 CysSer with the natural R-configuration at the glycerol 2-position. Pam3 CysSer peptide vaccines with natural configuration have been shown to be more efficient than the corresponding R/S diastereomers. In order to clarify whether the effect of the configuration of Pam3 Cys on the immune response also applies to glycopeptide vaccines, MUC1 glycopeptide-lipopeptide vaccines bearing either R- or R/S-configured Pam3 CysSerLys4 were compared for their immunological effects. In order to f…
Efficient expression of a Paenibacillus barcinonensis endoglucanase in Saccharomyces cerevisiae.
2011
Abstract The endoglucanase coded by celA (GenBank Access No. Y12512) from Paenibacillus barcinonensis, an enzyme with good characteristics for application on paper manufacture from agricultural fibers, was expressed in Saccharomyces cerevisiae by using different domains of the cell wall protein Pir4 as translational fusion partners, to achieve either secretion or cell wall retention of the recombinant enzyme. Given the presence of five potential N-glycosylation sites in the amino acid sequence coded by celA, the effect of glycosylation on the enzymatic activity of the recombinant enzyme was investigated by expressing the recombinant fusion proteins in both, standard and glycosylation-defici…
The baculovirus display technology--an evolving instrument for molecular screening and drug delivery.
2008
High throughput screening is a core technology in drug discovery. During the past decade, several strategies have been developed to screen (poly)peptide libraries for diverse applications including disease diagnosis and profiling, imaging, as well as therapy. The recently established baculovirus display vector system (BDVS) represents a eukaryotic screening platform that combines the positive attributes of both cell and virus-based display approaches, allowing presentation of complex polypeptides on cellular and viral surfaces. Compared to microbial display systems, the BDVS has the advantage of correct protein folding and post-translational modifications similar to those in mammals, facili…
Wharton’s Jelly Mesenchymal Stem Cells as Candidates for Beta Cells Regeneration: Extending the Differentiative and Immunomodulatory Benefits of Adul…
2010
Mesenchymal stem cells (MSC) are uniquely capable of crossing germinative layers borders (i.e. are able to differentiate towards ectoderm-, mesoderm- and endoderm-derived cytotypes) and are viewed as promising cells for regenerative medicine approaches in several diseases. Type I diabetes therapy should potentially benefit from such differentiated cells: the search for alternatives to organ/islet transplantation strategies via stem cells differentiation is an ongoing task, significant goals having been achieved in most experimental settings (e.g. insulin production and euglycaemia restoration), though caution is still needed to ensure safe and durable effects in vivo. MSC are obtainable in …
Vācu valoda kā svešvaloda. Latviešu un angļu valoda – palīgs vācu valodas apguvē
2016
Šajā darbā tiek pētītas jaunas alternatīvas mācību prakses vācu valodas kā svešvalodas mācīšanai pieaugušajiem un demonstrētas jaunas pieejas šo mācību optimizēšanai. Svarīgākais instruments ir vairāku valodu izmantošana mācību laikā, īpaši, latviešu un angļu. Šī darba mērķis ir pārbaudīt un pilnveidot pašlaik valodu apguves procesā izmantotās metodoloģijas piemērotību. Darbā izklāstīts pētāmā jautājuma teorētiskais pamatojums; praktiskajā daļā īpaši detalizēti tiek analizēti jauni, autora izstrādāti valodas mācību materiāli.
Development of a green fluorescent tagged strain of Aspergillus carbonarius to monitor fungal colonization in grapes.
2011
An enhanced green fluorescent protein has been used to tag an OTA-producing strain of Aspergillus carbonarius (W04-40) isolated from naturally infected grape berries. Transformation of the fungus was mediated by Agrobacterium tumefaciens. The most efficient transformation occurred when the co-cultivation was done with 104 conidia due to higher frequency of resistance colonies (894 per 104 conidia) and lower background obtained. To confirm the presence of the hph gene in hygromycin resistant colonies, 20 putative transformants were screened by PCR analysis. The hph gene was identified in all the transformants. Variation on the expression levels of the eGFP was detected among the transformant…
Filtering Real World Networks: A Correlation Analysis of Statistical Backbone Techniques
2023
Networks are an invaluable tool for representing and understanding complex systems. They offer a wide range of applications, including identifying crucial nodes, uncovering communities, and exploring network formation. However, when dealing with large networks, the computational challenge can be overwhelming. Fortunately, researchers have developed several techniques to address this issue by reducing network size while preserving its fundamental properties [1-9]. To achieve this goal, two main approaches have emerged: structural and statistical methods. Structural methods aim to keep a set of topological features of the network while reducing its size. In contrast, statistical methods elimi…
NetBone: A Python Package for Extracting Backbones of Weighted Networks
2023
NetBone is a new open-source Python package designed to simplify analyzing complex networks. With a wide range of techniques available, Net-Bone allows researchers to extract the backbone of a network while preserving its essential structure. The package includes nine structural methods and five statistical techniques, offering users a comprehensive solution to network analysis. It is user-friendly and straightforward to use, with easy installation. The package accepts different types of inputs, including data frames or Networkx graphs, and provides evaluation measures for comparative purposes. Additionally, NetBone offers an option to generate plots. Its versatility makes it a valuable too…
Versatile Electrochemical C-H Amination via Zincke Intermediates.
2015
Simply by applying electricity, the amination reaction of a broad variety of arenes, heteroarenes, and benzylic substrates is achieved. Pyridine serves as the nitrogen source and the intermediate cationic species are well-protected from over-oxidation.