Search results for "techniques"
showing 10 items of 4426 documents
The alhambra photometric system
2010
Aparicio Villegas, Teresa et al.
In vitro differentiation of murine hematopoietic progenitor cells toward the myeloid lineage occurs in response to Staphylococcus aureus and yeast sp…
2013
We have studied the effect of inactivated microbial stimuli (Candida albicans, Candida glabrata, Saccharomyces boulardii, and Staphylococcus aureus) on the in vitro differentiation of lineage negative (Lin−) hematopoietic progenitor mouse cells. Purified Lin− progenitors were co-cultured for 7 days with the stimuli, and cell differentiation was determined by flow cytometry analysis. All the stimuli assayed caused differentiation toward the myeloid lineage. S. boulardii and particularly C. glabrata were the stimuli that induced in a minor extent differentiation of Lin− cells, as the major population of differentiated cells corresponded to monocytes, whereas C. albicans and S. aureus induced …
Novel aspect of amphotericin B action: accumulation in human monocytes potentiates killing of phagocytosed Candida albicans.
1994
The influence of low doses of amphotericin B on the capacity of human monocytes to kill Candida albicans was investigated. Killing rates were quantified by a novel flow cytometric assay and were found to be 37% +/- 3% (standard error of the mean) after 3 h. Preincubation of monocytes for 6 to 20 h with low concentrations of amphotericin B (0.2 microgram/ml) resulted in a markedly augmented fungicidal capacity. Enhancement of killing was 80% +/- 11% (standard error of the mean) over that by the controls. This effect did not appear to be due to amphotericin B-dependent monocyte activation; the respiratory burst and expression of human leukocyte antigen-DR were unaltered, and no stimulation of…
Technical details of the hand-sewn and circular-stapled anastomosis in robot-assisted minimally invasive esophagectomy.
2020
SUMMARY The circular mechanical and hand-sewn intrathoracic anastomosis are most often used in robot-assisted minimally invasive esophagectomy (RAMIE). The aim of this study was to describe the technical details of both techniques that were pioneered in two high volume centers for RAMIE. A prospectively maintained database was used to identify patients with esophageal cancer who underwent RAMIE with intrathoracic anastomosis. The primary outcome was anastomotic leakage, which was analyzed using a moving average curve. For the hand-sewn anastomosis, video recordings were reviewed to evaluate number of sutures and distances between the anastomosis and the longitudinal staple line or gastric c…
Correcting the effect of stellar spots on ARIEL transmission spectra – II. The limb-darkening effect
2021
This paper is part of an effort to correct the transmission spectra of a transiting planet orbiting an active star. In Paper I (Cracchiolo et al. 2020) we have demonstrated a methodology to minimize the potential bias induced by unocculted star spots on the transmission spectrum, assuming a spot model parameterized by filling factor and temperature. In this work we introduce the limb darkening effect, therefore the position of the spot in the stellar disk and the impact parameter of the transiting planet now play a key role. The method is tested on simulations of planetary transits of three representative kinds of planetary systems, at ARIEL resolution. We find that a realistic treatment of…
Multi-epoch VLTI-PIONIER imaging of the supergiant V766 Cen
2017
Context. The star V766 Cen (=HR 5171A) was originally classified as a yellow hypergiant but lately found to more likely be a 27-36 M red supergiant (RSG). Recent observations indicated a close eclipsing companion in the contact or common-envelope phase. Aims. Here, we aim at imaging observations of V766 Cen to confirm the presence of the close companion. Methods. We used near-infrared H-band aperture synthesis imaging at three epochs in 2014, 2016, and 2017, employing the PIONIER instrument at the Very Large Telescope Interferometer (VLTI). Results. The visibility data indicate a mean Rosseland angular diameter of 4.1 ± 0.8 mas, corresponding to a radius of 1575 ± 400 R. The data show an ex…
Sub-threshold signal processing in arrays of non-identical nanostructures
2011
Weak input signals are routinely processed by molecular-scaled biological networks composed of non-identical units that operate correctly in a noisy environment. In order to show that artificial nanostructures can mimic this behavior, we explore theoretically noise-assisted signal processing in arrays of metallic nanoparticles functionalized with organic ligands that act as tunneling junctions connecting the nanoparticle to the external electrodes. The electronic transfer through the nanostructure is based on the Coulomb blockade and tunneling effects. Because of the fabrication uncertainties, these nanostructures are expected to show a high variability in their physical characteristics and…
Pathway analysis of high-throughput biological data within a Bayesian network framework
2011
Abstract Motivation: Most current approaches to high-throughput biological data (HTBD) analysis either perform individual gene/protein analysis or, gene/protein set enrichment analysis for a list of biologically relevant molecules. Bayesian Networks (BNs) capture linear and non-linear interactions, handle stochastic events accounting for noise, and focus on local interactions, which can be related to causal inference. Here, we describe for the first time an algorithm that models biological pathways as BNs and identifies pathways that best explain given HTBD by scoring fitness of each network. Results: Proposed method takes into account the connectivity and relatedness between nodes of the p…
Influence of inorganic pyrophosphate on the kinetics of muscle pyruvate kinase: a simple nonallosteric feedback model.
2002
Potassium pyrophosphate was used instead of ATP as a model ligand for magnesium cation for the study of effector influence on the kinetics of pyruvate kinase muscle isozyme M1. The pyruvate kinase activation by low concentration of pyrophosphate and inhibition by high concentration of pyrophosphate was considered to be the result of reversible reactions of magnesium cation with pyrophosphate, ADP, ATP, and PEP. The apparent Km and Vm or in some cases the pseudo-first order reaction rate constant (instead of Km and Vm) of pyruvate kinase at any given pyrophosphate concentration were analysed as a function of concentration of free magnesium cation and its complexes with all ligands present in…
Coupled variable selection for regression modeling of complex treatment patterns in a clinical cancer registry.
2013
For determining a manageable set of covariates potentially influential with respect to a time-to-event endpoint, Cox proportional hazards models can be combined with variable selection techniques, such as stepwise forward selection or backward elimination based on p-values, or regularized regression techniques such as component-wise boosting. Cox regression models have also been adapted for dealing with more complex event patterns, for example, for competing risks settings with separate, cause-specific hazard models for each event type, or for determining the prognostic effect pattern of a variable over different landmark times, with one conditional survival model for each landmark. Motivat…