Search results for "techniques"

Comparative analysis of production and purification of homo- and hetero-polysaccharides produced by lactic acid bacteria

2013

2 figuras, 2 tablas

An impaired alveolar-capillary barrier in vitro : effect of proinflammatory cytokines and consequences on nanocarrier interaction.

2009

The alveolar region of the lung is an important target for drug and gene delivery approaches. Treatment with drugs is often necessary under pathophysiological conditions, in which there is acute inflammation of the target organ. Therefore, in vitro models of the alveolar-capillary barrier, which mimic inflammatory conditions in the alveolar region, would be useful to analyse and predict effects of novel drugs on healthy or inflamed tissues. The epithelial cell line H441 was cultivated with primary isolated human pulmonary microvascular endothelial cells (HPMECs) or the endothelial cell line ISO-HAS-1 on opposite sides of a permeable filter support under physiological and inflammatory condi…

The ancient urban agroforestry systems of the Conca D'Oro (Palermo, Italy) need protection to defend the city

2019

The favorable environmental conditions together with the ancient agronomic practices, e.g. the irrigation systems introduced by the Arabs, have made the plain surrounding Palermo, known as Conca d’Oro, the unique agriculture landscape famous worldwide. Several tree and vegetable species have spread throughout in time but the new crops, instead of replacing the others, were often integrated exploiting the different heights of the tree canopies. The result was the creation of complex agroecosystems characterized by orchards with several layers of trees (as walnut, loquat, citrus) and empty spaces where the farmers cultivated vegetables. The grass fed the animals in the stables while manure wa…

Bioinorganic Chemistry Chapter 2

2018

Tema 2 de la part de Bioinorgànica de l'assignatura Química Inorgànica Avançada Chapter 2 of the Bioinorganic part of the subject Advanced Inorganic Chemistry

Inside Back Cover: Microarray Analysis of Antibodies Induced with Synthetic Antitumor Vaccines: Specificity against Diverse Mucin Core Structures (Ch…

2017

Changes of morphogenic competence in mature Pinus sylvestris L. buds in vitro.

2002

The effects of season and cold storage on morphogenic competence in mature Pinus sylvestris buds were investigated. Peroxidase and polyphenol oxidase activity were measured as markers of oxidative metabolism. No growth in vitro was observed on explants detached from the end of January until the beginning of March. Brachioblasts, each with a couple of needles, formed on 11 % of the buds without macrostrobili that were detached in early April and introduced immediately into culture. Of the explants detached in late July, 15 % formed shoots with brachioblasts and needles. The lowest activity of peroxidase and polyphenol oxidase in pine buds was observed from the end of April until the beginnin…

Neuropsychological underpinnings of the dynamics of bipolar disorder

2015

Although we have gained enormous insights into neurobiological and psychological underpinnings of bipolar disorder (BD) symptoms, our knowledge concerning pathogenic mechanisms initiating recurrent affective episodes is still fragmentary. Previous research has highlighted the role of significant life events and social rhythm in recurrent episodes of mania and depression. However, most studies share the drawback of retrospective self-report data, which are prone to recall biases and limited introspective abilities. Therefore, more objective data, such as neuropsychological and neurobiological measures are needed to further unravel the pathogenic mechanisms of the dynamics of bipolar disorder…

An interactive surrogate-based method for computationally expensive multiobjective optimisation

2019

Many disciplines involve computationally expensive multiobjective optimisation problems. Surrogate-based methods are commonly used in the literature to alleviate the computational cost. In this paper, we develop an interactive surrogate-based method called SURROGATE-ASF to solve computationally expensive multiobjective optimisation problems. This method employs preference information of a decision-maker. Numerical results demonstrate that SURROGATE-ASF efficiently provides preferred solutions for a decision-maker. It can handle different types of problems involving for example multimodal objective functions and nonconvex and/or disconnected Pareto frontiers. peerReviewed

OPTIMIZING PREIMPLANTARY OFFER TROUGH PIEZOSURGERY TECHNIQUES

2016

Introduction. In most of the cases, duet o post-extractional atrophy the bone offer is insufficent for placing implants. Piezosurgery splitting techniques represent the modern and non-invasive alternative used for ridge remodeling, sinus lift and other surgical bone preservation maneuvres. Purpose. The purpose of this study is to present new surgical techniques that radically simplify maxillary sinus interventation, avoiding membrane perforation and new splitting tecniques in advanced bone and ridge atrophy. Material and method. In this study, sinus lifting was performed using piezo-elevation of the membrane and the pressure ogenerated by the saline solution. Out of 67 patients, 20 have had…

Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture

2003

Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary …