Search results for "techniques"
showing 10 items of 4426 documents
Performance of $b$-Jet Identification in the ATLAS Experiment
2016
We thank CERN for the very successful operation of the LHC, as well as the support staff from our institutions without whom ATLAS could not be operated efficiently. We acknowledge the support of ANPCyT, Argentina; YerPhI, Armenia; ARC, Australia; BMWFW and FWF, Austria; ANAS, Azerbaijan; SSTC, Belarus; CNPq and FAPESP, Brazil; NSERC, NRC and CFI, Canada; CERN; CONICYT, Chile; CAS, MOST and NSFC, China; COLCIENCIAS, Colombia; MSMT CR, MPO CR and VSC CR, Czech Republic; DNRF, DNSRC and Lundbeck Foundation, Denmark; IN2P3-CNRS, CEA-DSM/IRFU, France; GNSF, Georgia; BMBF, HGF, and MPG, Germany; GSRT, Greece; RGC, Hong Kong SAR, China; ISF, I-CORE and Benoziyo Center, Israel; INFN, Italy; MEXT an…
Leishmania tarentolae and Leishmania infantum in humans, dogs and cats in the Pelagie archipelago, southern Italy.
2021
Visceral leishmaniasis (VL) caused by Leishmania infantum is endemic in the Mediterranean basin with most of the infected human patients remaining asymptomatic. Recently, the saurian-associated Leishmania tarentolae was detected in human blood donors and in sheltered dogs. The circulation of L. infantum and L. tarentolae was investigated in humans, dogs and cats living in the Pelagie islands (Sicily, Italy) by multiple serological and molecular testing. Human serum samples (n = 346) were tested to assess the exposure to L. infantum by immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) and to L. tarentolae by IFAT. Meanwhile, sera from do…
Drāmas paņēmieni angļu valodas runāšanas trauksmes mazināšanai 6. klasē
2016
Lielākā daļa cilvēku, kuri mācās angļu valodu kā svešvalodu, saskaras ar angļu valodas runāšanas trauksmi. Tā negatīvi ietekmē gan skolēnu sniegumu, gan attieksmi. Pētījums tika izstrādāts ar mērķi pārbaudīt, kā drāmas paņēmieni samazināja skolēnu angļu valodas runāšanas trauksmi 6.klasē. Angļu valodas stundās 6.klasē tika izmantotas dažādas drāmas aktivitātes. Darbā tika lietotas tādas datu vākšanas metodes kā diagnosticējošs tests (pirms un pēc drāmas paņēmienu ieviešanas), intervija, novērošana un atgriezeniskā saite. Diagnosticējošā testa rezultāti norādīja, ka skolēni saskārās ar angļu valodas runāšanas trauksmes pazīmēm. Drāmas paņēmienu izmantojums palielināja skolēnu aktivitāti angļ…
Il terremoto del 1823 in Sicilia settentrionale: danni e ricostruzioni
2016
Il 5 marzo 1823 si verificò un terremoto che investì gran parte della Sicilia settentrionale. Numerosi danni si verificarono a Palermo, nelle aree limitrofe a sud e ovest della capitale, in alcuni centri delle Madonie e nelle zone situate lungo il litorale e l’immediato entroterra tirrenico da Palermo a Patti (in particolare a Naso). Il contributo intende indagare, attraverso lo studio delle opere degli autori coevi al sisma e soprattutto di una copiosa documentazione archivistica, le modalità attraverso le quali ci si raffrontò con i problemi determinati dal terremoto, dalla gestione dell'emergenza, all'analisi dei danni e delle successive ricostruzioni. Dal quadro tracciato emerge in part…
Terremoto e architettura storica. Palermo e il sisma del 1726.
2015
On September 1st 1726 Palermo was hit by a violent earthquake. Although it did not bring about the destruction of the city, the earthquake caused widespread and extensive damage to the architectural heritage. The local insti- tutions carried out a series of actions involving representatives of the technical culture. These actions affected the subsequent appearance of the urban pattern and influenced the construction methods of the time. Crossing the data taken from chronicles of the time, from maps produced after the earthquake and above all copious archival documents, the research aims to reconstruct the state in which Palermo was after that tragic event, highlighting a number of aspects. …
Design and experimental validation of a generic model for combinatorial assembly of DNA tiles into 1D-structures.
2010
Abstract Background Quantitative modeling of the self-assembly of DNA tiles leading either to defined end-products or distribution of biopolymers is of practical importance for biotechnology and synthetic biology. Methods The combinatorial process describing tile assembly was implemented into a generic algorithm allowing quantitative description of the population of significant species accumulating during the reaction course. Experimental formation and characterization by optical and electrophoresis approaches of copolymers resulting from the self-assembly of a limited number of half-complementary tiles were used to define and validate generic rules allowing definition of model parameters. …
Co-treatment of landfill leachate in laboratory-scale sequencing batch reactors: analysis of system performance and biomass activity by means of resp…
2014
Aged or mature leachate, produced by old landfills, can be very refractory; for this reason mature leachate is difficult to treat alone, but it can be co-treated with sewage or domestic wastewater. The aim of the study was to investigate the feasibility of leachate co-treatment with synthetic wastewater, in terms of process performance and biomass activity, by means of respirometric techniques. Two sequencing batch reactors (SBRs), named SBR1 and SBR2, were fed with synthetic wastewater and two different percentages of landfill leachate (respectively 10% and 50% v v−1 in SBR1 and SBR2). The results showed good chemical oxygen demand (COD) removal efficiency for both reactors, with average C…
Measurement of the SMC muon beam polarisation using the asymmetry in the elastic scattering off polarised electrons
2000
A muon beam polarimeter was built for the SMC experiment at the CERN SPS, for beam energies of 100 and 190 GeV. The beam polarisation is determined from the asymmetry in the elastic scattering off the polarised electrons of a ferromagnetic target whose magnetisation is periodically reversed. At muon energies of 100 and 190 GeV the measured polarisation is P-mu = -0.80 +/- 0.03 (stat.) +/- 0.02 (syst.) and P-mu = - 0.797 +/- 0.011 (stat.) +/- 0.012 (syst.), respectively. These results agree with measurements of the beam polarisation using a shape analysis of the decay positron energy spectrum. (C) 2000 Elsevier Science B.V. All rights reserved.
Development of an in vitro neuroblastoma 3D model and its application for sterigmatocystin-induced cytotoxicity testing
2021
Abstract Given the increasing importance of establishing better risk assessments for mycotoxins, novel in vitro tools for the evaluation of their toxicity are mandatory. In this study, an in vitro 3D spheroid model from SH-SY5Y cells, a human neuroblastoma cell line, was developed, optimized and characterized to test the cytotoxic effects caused by the mycotoxin sterigmatocystin (STE). STE induced a concentration- and time-dependent cell viability decrease in spheroids. Spheroids displayed cell disaggregation after STE exposure, increasing in a dose-dependent manner and over time. STE also induced apoptosis as confirmed by immunofluorescence staining and Western blot. Following the decrease…
Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets.
2006
Mesenchymal cells in the developing pancreas express the neural stem cell marker nestin and the transcription factor islet-1 (Isl-1). Using defined culture conditions we isolated on a single cell basis nestin producing cells from human pancreatic islets. These cells were immortalized with lentiviral vectors coding for telomerase and mBmi. They are positive for Isl-1 and nestin and have the potential to adopt a pancreatic endocrine phenotype with expression of critical transcription factors including Ipf-1, Isl-1, Ngn-3, Pax4, Pax6, Nkx2.2, and Nkx6.1 as well as the islet hormones insulin, glucagon, and somatostatin. In addition, they can be differentiated into human albumin producing cells …