Search results for "techno"

showing 10 items of 31274 documents

Occlusion-derived baculovirus: interaction with human cells and evaluation of the envelope protein P74 as a surface display platform.

2008

To develop complementary baculovirus-based tools for gene delivery and display technologies, the interaction of occlusion-derived baculovirus (ODV) with human cells, and the functionality of the P74 ODV envelope protein for display of the IgG-binding Z domains (ZZP74) were evaluated. The cellular binding of ODV was concentration-dependent and saturable. Only minority of the bound virions were internalized at both 37 and 4 degrees C, suggesting usage of direct membrane fusion as the entry mode. The intracellular transport of ODV was confined in vesicular structures peripheral to the plasma membrane, impeding subsequent nuclear entry and transgene expression. Transduction of ODV was not rescu…

virusesBlotting WesternVirus AttachmentBioengineeringBiologyGene deliverySpodopteraApplied Microbiology and BiotechnologyCell Linechemistry.chemical_compoundTransduction (genetics)Viral envelopeMicroscopy Electron TransmissionViral Envelope ProteinsCell Line TumorAnimalsHumansMicroscopy ConfocalfungiLipid bilayer fusionSodium butyrateGeneral MedicineMolecular biologyFusion proteinCell biologyNocodazolechemistryCell cultureElectrophoresis Polyacrylamide GelBaculoviridaeBiotechnologyJournal of biotechnology
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Internalization of novel non-viral vector TAT-streptavidin into human cells

2007

BMC Biotechnology, 7 (1)

virusesEndocytic cyclePROTEINS + POLYPEPTIDES (BIOCHEMISTRY)02 engineering and technologyei-virusperäinen vektoriProtein EngineeringgeeniterapiaPost Transductionchemistry.chemical_compoundTHERAPIES + THERAPEUTICS (MEDICINE)Drug Delivery SystemsLääketieteen bioteknologia - Medical biotechnologyInternalizationmedia_commoninfo:eu-repo/classification/ddc/5700303 health sciencesPinocytosisNocodazoleVEKTOREN (GENETISCHE TECHNIKEN)021001 nanoscience & nanotechnologyLife sciencesCell biologyEndosomal EscapeBiotinylationGene Products tatVirusesVECTORS (GENETIC TECHNIQUES)VEKTOREN (GENETISCHE TECHNIKEN); THERAPIEN + THERAPEUTIK (MEDIZIN); PROTEINE + POLYPEPTIDE (BIOCHEMIE); VECTORS (GENETIC TECHNIQUES); THERAPIES + THERAPEUTICS (MEDICINE); PROTEINS + POLYPEPTIDES (BIOCHEMISTRY)0210 nano-technologyTHERAPIEN + THERAPEUTIK (MEDIZIN)BiotechnologyResearch ArticleStreptavidinEndosomeImmunoelectron microscopymedia_common.quotation_subjectRecombinant Fusion Proteinslcsh:BiotechnologyGenetic VectorsBiologyEndocytosis03 medical and health sciencesstreptavidiiniddc:570lcsh:TP248.13-248.65HumansEndosomal Marker030304 developmental biologyMolecular biologyEndocytic VesiclechemistryStreptavidinTATPROTEINE + POLYPEPTIDE (BIOCHEMIE)HeLa CellsBMC Biotechnology
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Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.

2004

Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…

virusesHepatitis C virusAmino Acid MotifsBiomedical EngineeringNicotiana benthamianaBioengineeringmedicine.disease_causeAntibodies ViralVirus ReplicationApplied Microbiology and BiotechnologyVirusPlant VirusesTombusviruschemistry.chemical_compoundRNA polymerasePlant virusTobaccomedicinePlant DiseasesGeneticsbiologyfungifood and beveragesbiology.organism_classificationPlants Genetically ModifiedRNA-Dependent RNA PolymeraseVirologyTombusviridaechemistryViral replicationMolecular MedicineSingle-Chain AntibodiesBiotechnologyNature biotechnology
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Molecular Characterization of a Variant of Bacillus anthracis-Specific Phage AP50 with Improved Bacteriolytic Activity▿ †

2008

ABSTRACT The genome sequence of a Bacillus anthracis -specific clear plaque mutant phage, AP50c, contains 31 open reading frames spanning 14,398 bp, has two mutations compared to wild-type AP50t, and has a colinear genome architecture highly similar to that of gram-positive Tectiviridae phages. Spontaneous AP50c-resistant B. anthracis mutants exhibit a mucoid colony phenotype.

virusesMutantMolecular Sequence DataMutation MissenseGenetics and Molecular BiologyBacillus PhagesGenome ViralViral Plaque AssayApplied Microbiology and BiotechnologySyntenyBacteriophageBacteriolysisGene OrderPoint MutationBacillus (shape)Whole genome sequencingGeneticsEcologybiologyBase SequenceTectivirusVirionSequence Analysis DNAbiology.organism_classificationBacillus anthracisOpen reading frameBacillus anthracisDNA ViralTectiviridaeFood ScienceBiotechnologyTectiviridae
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The ALICE experiment at the CERN LHC

2008

Journal of Instrumentation 3(08), S08002 (2008). doi:10.1088/1748-0221/3/08/S08002

visible and IR photonsLiquid detectorshigh energyPhotonPhysics::Instrumentation and DetectorsTransition radiation detectorsTiming detectors01 natural sciencesOverall mechanics designParticle identificationSoftware architecturesParticle identification methodsGaseous detectorscluster findingDetector cooling and thermo-stabilizationDetector groundingParticle tracking detectors[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]Special cablesDetector alignment and calibration methodsDetectors and Experimental TechniquesNuclear ExperimentVoltage distributions.Photon detectors for UVInstrumentationMathematical PhysicsQuantum chromodynamicsPhysicsLarge Hadron ColliderSpectrometersPhysicsDetectorcalibration and fitting methodsTransition radiation detectorScintillatorsData processing methodsAnalysis and statistical methodsData reduction methodsParticle physicsCherenkov and transition radiationTime projection chambers610dE/dx detectorsNuclear physicsCalorimetersPattern recognitionGamma detectors0103 physical sciencesddc:610Solid state detectors010306 general physicsMuonInstrumentation for heavy-ion acceleratorsSpectrometerLarge detector systems for particle and astroparticle physics010308 nuclear & particles physicsCERN; LHC; ALICE; heavy ion; QGPCherenkov detectorsComputingVoltage distributionsManufacturingscintillation and light emission processesanalysis and statistical methods; calorimeters; cherenkov and transition radiation; cherenkov detectors; computing; data processing methods; data reduction methods; de/dx detectors; detector alignment and calibration methods; detector cooling and thermo-stabilization; detector design and construction technologies and materials; detector grounding; gamma detectors; gaseous detectors; instrumentation for heavy-ion accelerators; instrumentation for particle accelerators and storage rings - high energy; large detector systems for particle and astroparticle physics; liquid detectors; manufacturing; overall mechanics design; particle identification methods; particle tracking detectors; pattern recognition; cluster finding; calibration and fitting methods; photon detectors for uv; visible and ir photons; scintillators; scintillation and light emission processes; simulation methods and programs; software architectures; solid state detectors; special cables; spectrometers; time projection chambers; timing detectors; transition radiation detectors; voltage distributionsInstrumentation for particle accelerators and storage ringsInstrumentation; Mathematical PhysicsHigh Energy Physics::ExperimentSimulation methods and programsDetector design and construction technologies and materials
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Conception en technologie CMOS d'un Système de Vision dédié à l'Imagerie Rapide et aux Traitements d'Images

2008

Our work presented in this thesis focuses on the design, testing and implementation of monolithics CMOS image smart sensors : The principle, performance and limitations. The hardware implementation of a vision smart system is the central link. HISIC is High Speed Image Capture with processing at pixel level. An experimental platform for instrumentation and evaluation of retina operators was conducted during this thesis. After a state of the smart sensors and CMOS retinas, the second part is dedicated to the study and design of the pixel image sensor HISIC. Two circuits were realized in CMOS technology. The first identied a new type of photo-detector, and the second, to create a prototype em…

vision artificielleimagerie rapiderecongurabilityprogrammability[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronicsparallélisme massifspeed imagingSystème embarquéartificial vision.image processing on focal planerecongurabilitéprogrammabilitéstandard CMOS technology[ SPI.NANO ] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsEmbedded systemtraitements d'images sur plan focalmassive paralleltechnologie standard CMOS
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AnatomySketch : An Extensible Open-Source Software Platform for Medical Image Analysis Algorithm Development

2021

AbstractThe development of medical image analysis algorithm is a complex process including the multiple sub-steps of model training, data visualization, human–computer interaction and graphical user interface (GUI) construction. To accelerate the development process, algorithm developers need a software tool to assist with all the sub-steps so that they can focus on the core function implementation. Especially, for the development of deep learning (DL) algorithms, a software tool supporting training data annotation and GUI construction is highly desired. In this work, we constructed AnatomySketch, an extensible open-source software platform with a friendly GUI and a flexible plugin interfac…

visualisointiihmisen ja tietokoneen vuorovaikutussyväoppiminenlääketiedetekoälyuser interactionimage annotationUser-Computer InterfaceArtificial Intelligencealgoritmitihminen-konejärjestelmätHumansRadiology Nuclear Medicine and imagingRadiological and Ultrasound TechnologyAnatomySketchalgorithm developmenttietokoneohjelmatdeep learningMagnetic Resonance ImagingComputer Science Applicationskoneoppiminenkuva-analyysiohjelmointimedical image analysisSoftwareAlgorithms
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Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging

2018

International audience; Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200pM of beta-ionone) and in molecular weight of …

volatile organic compoundConformational change[SDV.BIO]Life Sciences [q-bio]/BiotechnologyOdorant bindingBiomedical EngineeringBiophysicsBiosensing Techniques02 engineering and technologyReceptors Odorant01 natural sciencesHexanal[SPI]Engineering Sciences [physics]chemistry.chemical_compoundElectrochemistryAnimalsVolatile organic compoundComputingMilieux_MISCELLANEOUSDetection limitchemistry.chemical_classificationVolatile Organic CompoundsChromatographyChemistry010401 analytical chemistryGeneral MedicineRepeatabilitySurface Plasmon Resonance021001 nanoscience & nanotechnologyRats0104 chemical sciencesSmellsurface plasmon resonance imagingofactory biosensor0210 nano-technologySelectivityBiosensorodorant binding proteinsBiotechnologyBiosensors and Bioelectronics
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Impact of Etna’s volcanic emission on major ions and trace elements composition of the atmospheric deposition

2022

Mt. Etna, on the eastern coast of Sicily (Italy), is one of the most active volcanoes on the planet and it is widely recognized as a big source of volcanic gases (e.g., CO2 and SO2), halogens, and a lot of trace elements, to the atmosphere in the Mediterranean region. Especially during eruptive periods, Etna’s emissions can be dispersed over long distances and cover wide areas. A group of trace elements has been recently brought to attention for their possible environmental and human health impacts, the Technology-critical elements. The current knowledge about their geochemical cycles is still scarce, nevertheless, recent studies (Brugnone et al., 2020) evidenced a contribution from the vol…

volcanic emissions technology-critical elements human healthSettore GEO/08 - Geochimica E Vulcanologia
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Electrical conductivity of glass fiber-reinforced plastic with nanomodified matrix for damage diagnostic

2021

The electrical conductivity of glass fiber-reinforced plastic (GFRP) with epoxy matrix modified by multiwall carbon nanotubes (MWCNT) was studied. The electrical conductivity of nanomodified lamina and multi-layered GFRP was investigated on several levels using a structural approach. Components of the electrical conductivity tensor for unidirectional-reinforced monolayer were calculated similarly as in micromechanics using the conductivity of the nanomodified matrix. The electrical conductivity of multilayer composite was calculated using laminate theory and compared with values measured experimentally for various fiber orientation angles. Calculated and experimental data were in good agree…

voltage distributionTechnologyMaterials scienceGlass fiberCarbon nanotubeConductivityArticlelaw.inventionFracture toughnessmicromechanicsElectrical resistance and conductancelawGeneral Materials ScienceComposite materialinterlaminar fractureMicroscopyQC120-168.85carbon nanotubeselectrical conductivityTQH201-278.5glass fiber reinforced plasticMicromechanicsFracture mechanicsdamage diagnosticFibre-reinforced plasticEngineering (General). Civil engineering (General)TK1-9971Descriptive and experimental mechanicsElectrical engineering. Electronics. Nuclear engineeringTA1-2040
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