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FRI0030 Anti-TNF-α Antibody Targeted To Inflamed Synovial Tissue for The Treatment of Rheumatoid Arthritis
2016
Background TNF-α neutralizing molecules represent one of the most efficient therapeutic approaches to control inflammation in rheumatoid arthritis (RA). The widespread distribution in the body induces the inhibition of TNF-α in all the tissues, requesting the use of high dose of this expensive drug. Another problem that has not yet been solved in the management of RA patients is how to reduce and possibly avoid the side effects, particularly the increased risk of common and opportunistic infections, which may be associated with long-term administration of these therapeutic drugs. Objectives The aim of the present investigation was to show that a recombinant protein obtained by fusing a syno…
Activation of astroglial phospholipase D activity by phorbol ester involves ARF and Rho proteins.
2000
Primary cultures of rat cortical astrocytes express phospholipase D (PLD) isoforms 1 and 2 as determined by RT-PCR and Western blot. Basal PLD activity was strongly (10-fold) increased by 4beta-phorbol-12beta,13alpha-dibutyrate (PDB) (EC(50): 56 nM), an effect which was inhibited by Ro 31-8220 (0.1-1 microM), an inhibitor of protein kinase C (PKC), and by brefeldin A (10-100 microg/ml), an inhibitor of ADP-ribosylating factor (ARF) activation. Pretreatment of the cultures with Clostridium difficile toxin B-10463 (0.1-1 ng/ml), which inactivates small G proteins of the Rho family, led to a breakdown of the astroglial cytoskeleton; concomitantly, PLD activation by PDB was reduced by up to 50%…
Inhibition of Protein Isoprenylation Impairs Rho-Regulated Early Cellular Response to Genotoxic Stress
2000
Activation of c-Jun N-terminal kinases (JNKs) and nuclear factor-kappaB (NF-kappaB) are early cellular responses to genotoxic stress involved in the regulation of gene expression. Pretreatment of cells with the hydroxymethyl glutaryl-CoA reductase inhibitor lovastatin blocked stimulation of JNK1 activity by UV irradiation and by treatment with the alkylating compound methyl methanesulfonate but did not affect activation of extracellular signal-regulated kinase 2 by UV light. Lovastatin also attenuated UV-induced degradation of the NF-kappaB inhibitor IkappaBalpha. The effects of lovastatin on UV-triggered stimulation of JNK1 as well as on IkappaBalpha degradation were reverted by cotreatmen…
Detection of natural killer T cells in mice infected with Rickettsia conorii.
2013
Little information is available regarding the role of natural killer T (NKT) cells during the early stage of Rickettsia conorii infection. Herein, C3H/HeN mice were infected with the Malish 7 strain of R. conorii. Splenocytes from these mice were analysed in the early stage of the infection by flow cytometry and compared with uninfected controls. Our results showed an increase in NKT cells in infected mice. Additionally, NKT interleukin (IL)-17(+) cells increased three days after infection, together with a concurrent decrease in the relative amount of NKT interferon (IFN)-γ(+) cells. We also confirmed a higher amount of NK IFN-γ(+) cells in infected mice. Taken together, our data showed tha…
Brevi riflessioni sul riparto della potestà legislativa in materia di diritto del lavoro tra lo Stato e la Regione Sicilia
2012
Scopo del lavoro è di individuare, esaminando la recente giurisprudenza della Corte costituzionale, quali conseguenze siano derivate sul riparto di competenza legislativa in materia di diritto del lavoro tra lo Stato e la regione siciliana in seguito alla riforma del titolo V della parte seconda della Costituzione. The article investigates which consequences has had the reform of title V, second part of the italian Constitution, in the legislative power allocation between State and Sicilian Region in labour law matter, focusing its attention on the recent development of the case law of the Constitutional Court.
Il sistema della profenolossidasi in Ciona intestinalis. Caratterizzazione e interazione tra fenolossidasi, perossinectina e superossido dismutasi Cu…
2013
Ritual and symbolism in Northern Irish parading culture
2002
Rappresentazioni del sacro femminile. I busti femminili "di tipo agrigentino" in contesto
2021
Il contributo esamina la categoria dei busti fittili femminili in ottica contestuale, concentrando l'attenzione su due casi tra i più significativi a oggi noti: il Santuario rupestre di Agrigento e il Santuario del cd. altare di Demetra a Lipari. L'esame comparato consente di evidenziare sia le ricorrenze sia le specifiche connotazioni che la formula assume nella pratica rituale di ciascun santuario. The contribution examines the category of female fictile busts from a contextual point of view, focusing attention on two of the most significant cases known to date: the rupestrian sanctuary of Agrigento and the sanctuary of the cd. altar of Demeter in Lipari. The comparative examination allow…
Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods.
2020
The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. …
Ruch pokojowy w RFN (Friedensbewegung) w pierwszej połowie lat osiemdziesiątych XX wieku
2017
W latach 80. XX wieku ruch pokojowy mobilizujący setki tysięcy osób w protestach przeciwko podwójnej uchwale NATO z grudnia 1979 r. był istotnym elementem zachodnioniemieckiej sceny politycznej. Wpływał na układ sił i konflikty wewnętrzne w socjaldemokracji niemieckiej (SPD), będąc największym oponentem polityki prowadzonej przez rząd kanclerza Helmuta Schmidta, przyczynił się do powstania Partii Zielonych, a jego działania doprowadziły do zaistnienia polemiki w związkach zawodowych i były bodźcem do dyskusji etycznych i religijnych w Kościele katolickim i protestanckim w RFN. Stał się również przedmiotem wielu kontrowersji oraz symbolem aktywności i siły społecznej.